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We narrowed to 5,292 results for: codon optimized

Showing: 3021 - 3040 of 5292 results
  1. pDONR_P2R-P3_R2-Turbo-NES-mVenus-STOP-L3

    Plasmid
    #127353
    Purpose
    gateway entry vector for making C-terminal TurboID-mVenus fusion with non-nuclear proteins
    Depositor
    Insert
    TurboID (BirA mutant)
    Use
    Gateway Cloning
    Tags
    GS linker, NES, V5, and mVenus
    Mutation
    Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…
    Promoter
    no promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. R4pGWB601_UBQ10p-miniTurbo-YFP-NLS

    Plasmid
    #127370
    Purpose
    Binary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Tags
    GS linker, NLS, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pCS2+ heiCas9

    Plasmid
    #186929
    Purpose
    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS
    Depositor
    Insert
    myc-flexible linker-oNLS-Cas9-oNLS
    Use
    CRISPR
    Tags
    myc-flexible linker-optimizedNLS and optimizedNLS
    Expression
    Mammalian
    Promoter
    sp6
    Available Since
    April 21, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pKSEe401R

    Plasmid
    #218532
    Purpose
    CRISPR/Cas9 genome editing vector pKSEe401R contains the maize codon-optimized Cas9 (zCas9) enhanced by the 5′-UTR fragment of OsMac3 and the pAtUBQ10-DsRED1-NOSt screening cassette in the backbone.
    Depositor
    Type
    Empty backbone
    Expression
    Plant
    Available Since
    Jan. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
  5. R4pGWB601_UBQ10p-miniTurbo-NES-YFP

    Plasmid
    #127369
    Purpose
    Binary vector for expressing cytosolic miniTurbo-YFP under the UBQ10 promoter in plants
    Depositor
    Insert
    miniTurbo (BirA mutant)
    Tags
    GS linker, NES, V5, and YFP
    Expression
    Plant
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Promoter
    Arabidopsis UBQ10 promoter
    Available Since
    Sept. 18, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pCRISPR-mcBEST

    Plasmid
    #209416
    Purpose
    Plasmid for multiplexed cytosine base editing in streptomycetes
    Depositor
    Inserts
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    csy4 from Pseudomonas aeruginosa PAO1
    Use
    CRISPR and Synthetic Biology
    Promoter
    base editing cassette: PtipA ; sgRNAs: PkasO* an…
    Available Since
    Dec. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  7. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
  8. pBUE411

    Plasmid
    #62200
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    OsU3 and Ubi
    Available Since
    April 29, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 3021 - 3040 of 5292 results