We narrowed to 3,800 results for: golden gate

1. miRFP670-TRF1

   Plasmid

   #227388

   Purpose

   Label telomere, no adhesion

   Depositor

   Clifford Brangwynne

   Article

   Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

   Insert

   Telomeric miRFP (TERF1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

   Plasmid

   #181792

   Purpose

   Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
   

   Expression

   Bacterial

   Mutation

   nAniI = K227M, F80K, L232K

   Promoter

   Lac and J23119

   Available Since

   March 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEM394 - mosTI unc-119 - PUPN - NNN - tbb-2

   Plasmid

   #182352

   Purpose

   Pan-neuronal expression cloning vector for mosTI(unc-119). Transgene inserted between Ultra pan neuronal (UPN) promoter and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pNP568

   Plasmid

   #222725

   Purpose

   pNP555 - IS621 RE-LE TBL-WT, DBL-WT bRNA in cis

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 bridge RNA
   

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pENTR-B/c

   Plasmid

   #227962

   Purpose

   Entry plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

   Depositor

   Alberto Carbonell

   Article

   Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

   Insert

   B/c
   

   Promoter

   None

   Available Since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_ancCas9d_Plasmid

   Plasmid

   #239444

   Purpose

   Expression plasmid codon-optimized for E. coli

   Depositor

   David Taylor

   Article

   Ocampo et al Nat Commun. 2025 Jan 7;16(1):457. doi: 1

   Insert

   ancCas9d
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNP1723

   Plasmid

   #222727

   Purpose

   sfGFP-ON BsaI Golden Gate (GG) Landing Pad for LD-RD and J23119-BsaI GG Landing Pad for bRNA (bRNA in trans expression)

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Type

   Empty backbone

   Tags

   sfGFP

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pNP2157

   Plasmid

   #222732

   Purpose

   pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (WT)
   

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAGC-Lb12-P1Bb

   Plasmid

   #231387

   Purpose

   For crRNA cloning with LbCas12a vectors for genome editing in Arabidopsis

   Depositor

   Qi-Jun Chen

   Article

   Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:

   Insert

   U6-29
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ131-tRNA2.0

    Plasmid

    #158393

    Purpose

    Golden Gate entry vector to express the 1st gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Without promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNP1047

    Plasmid

    #222726

    Purpose

    pNP555 - IS621 RE-LE TBL-T5, DBL-WT bRNA in cis

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 bridge RNA
    

    Tags

    sfGFP

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pNP2270

    Plasmid

    #222733

    Purpose

    pNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Helper (lacZ)
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pYPQ133B2.0

    Plasmid

    #99892

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pY2ShHELIX_sgRNA_entry (CJT30)

    Plasmid

    #181781

    Purpose

    Expresses Y2-ShHELIX containing a nicking Y2 I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    Y2-nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Expression

    Bacterial

    Mutation

    Y2-nAniI = F13Y, S111Y, K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ142-ZmUbi-tRNA

    Plasmid

    #158578

    Purpose

    Gateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 and pYPQ132-tRNA2.0; assembly of 2 gRNAs

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Maize ubiquitin 1

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLaNGo11-7

    Plasmid

    #126055

    Purpose

    A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Inserts

    chloroplastic FNR transit peptide
    ccdB cassette (BsaI)
    GFP11x7 (5 aa linker)
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ132-tRNA2.0

    Plasmid

    #158394

    Purpose

    Golden Gate entry vector to express the 2nd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    Without promoter

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYPQ134B2.0

    Plasmid

    #167158

    Purpose

    Golden Gate entry vector to express the 4th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    AtU3

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pM-mClover3

    Plasmid

    #228624

    Purpose

    Mammalian expression of mClover3 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

    Insert

    mClover3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Level 1 P1 OsActinP:Hygint:NosT

    Plasmid

    #165423

    Purpose

    GoldenGate (MoClo) Level 1 Position 1 (Reverse) hygromycin selection cassette

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.

    Insert

    Os Actin promoter :: Hygromycin resistance gene (hpt) containing CAT1 intron :: NosTerminator
    

    Use

    Synthetic Biology; L1 p1(r) golden gate (moclo) h…

    Promoter

    Os Actin promoter :: Hygromycin resistance gene (…

    Available Since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge