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pMIHP-16191 AU1.VSVG.V5

Plasmid

#241500

Purpose

AU1.VSVG.V5 Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Publication

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

AU1, V5, and VSVG

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16273 Flag.S.VSVG

Plasmid

#241503

Purpose

Flag.S.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Publication

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

Flag, S, and VSVG

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16285 HA.Ollas.VSVG

Plasmid

#241504

Purpose

HA.Ollas.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Publication

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

HA, Ollas, and VSVG

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMIHP-16287 HA.S.VSVG

Plasmid

#241505

Purpose

HA.S.VSVG Flow Code Plasmid This plasmid is to be used with mRNAs.

Depositor

Adrian Liston

Publication

Burton et al Immunity. 2024 Jul 9;57(7):1586-1602.e10

Type

Empty backbone

Use

Retroviral

Tags

HA, S, and VSVG

Available since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3

Plasmid

#240233

Purpose

Expression of Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

Myc-BirA*G3

Expression

Insect

Promoter

MT

Available since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-sfGFP-TurboID-ER

Plasmid

#240230

Purpose

Expression of ER-localized sfGFP-TurboID under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

MT

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-sfGFP-TurboID-ER

Plasmid

#240232

Purpose

Expression of ER-localized sfGFP-TurboID under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

BIP-sfGFP-TurboID-KDEL

Expression

Insect

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-Myc-BirA*G3-ER

Plasmid

#240234

Purpose

Expression of ER-localized Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

BiP-Myc-BirA*G3-KDEL

Expression

Insect

Promoter

MT

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-spatzle-HA

Plasmid

#240235

Purpose

Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

spatzle (spz Fly)

Tags

HA-spGFP11

Expression

Insect

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-hhN-HA

Plasmid

#240236

Purpose

Expression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

hedgehog N-terminal fragment (hh Fly)

Tags

HA-spGFP11

Expression

Insect

Mutation

aa 1-257

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pUAS-CG6867-sfGFP

Plasmid

#240237

Purpose

Expression of CG6867-sfGFP under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

CG6867 (CG6867 Fly)

Tags

sfGFP

Expression

Insect

Promoter

UAS

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_HA-spGFP11

Plasmid

#240224

Purpose

Gateway entry clone for cloning in insert sequences by gibson assembly to create a C-terminal HA-spGFP11 tag. No ATG start codon.

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Type

Empty backbone

Use

Gateway shuttle vector

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pPV-C1-crRNA(DMD#20_DMD#23)-EF1a-BA

Plasmid

#204620

Purpose

Expression vector of crRNA (DMD#20) targeting the dystrophin intron 44 just upstream of exon 45, and crRNA(DMD#23)targeting the dystrophin intron 55 just downstream of exon 55. Blasticidin selectable.

Depositor

Akitsu Hotta

Publication

Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

Insert

crRNA#20 (targeting DMD intron44) and crRNA#23 (targeting DMD intron 55)

Use

CRISPR

Promoter

U6

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRS193

Plasmid

#197143

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (EF1a promoter version)

Depositor

Nozomu Yachie

Publication

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS138

Plasmid

#197142

Purpose

scCloneSelect C-to-T DNA barcode cloning backbone (CMV promoter version)

Depositor

Nozomu Yachie

Publication

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS140

Plasmid

#197141

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (CMV promoter version)

Depositor

Nozomu Yachie

Publication

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

CMV

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRS191

Plasmid

#197140

Purpose

scCloneSelect C-to-T reporter encoding BC-C1 barcode (EF1a promoter version)

Depositor

Nozomu Yachie

Publication

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Mutation

EGFP(M1V)

Promoter

EF1a

Available since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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pNM1325

Plasmid

#197144

Purpose

CAGp-Target-AID-2A-BlastR for PiggyBac genomic integration

Depositor

Nozomu Yachie

Publication

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

SpCas9-PmCDA1-UGI

Use

CRISPR

Expression

Mammalian

Mutation

SpCas9(D10A)

Promoter

CAG

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-146

Plasmid

#197136

Purpose

CloneSelect Indel reporter encoding BC-del1 barcode

Depositor

Nozomu Yachie

Publication

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-CS-196

Plasmid

#197138

Purpose

CloneSelect C-to-T mCherry (M9A) ATG control reporter encoding BC-C1 barcode

Depositor

Nozomu Yachie

Publication

Ishiguro et al Nat Biotechnol. 2025 May 21. doi: 10.103

Insert

mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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