We narrowed to 4,010 results for: biorxiv

1. pLX-TRE-dCas9-KRAB-MeCP2-BSD

   Plasmid

   #140690

   Purpose

   Inducible knockdown of gene expression in human cells for pooled scRNA-seq experiments

   Depositor

   Andrea Califano

   Article

   Tan et al bioRxiv 2021.06.28.449297

   Insert

   Cas9m4-KRAB-MeCP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOT_7-lenti-EFS-SS1-BBz-2A-puro

   Plasmid

   #217990

   Purpose

   Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Xue et al bioRxiv 2024.11.27.625752

   Insert

   SS1-BBz CAR
   

   Use

   Lentiviral

   Available Since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

   Plasmid

   #207664

   Purpose

   Neuropeptide degradation

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   NEP (MME Human)
   

   Use

   AAV and Cre/Lox

   Tags

   mRUBY3

   Mutation

   Addition of signaling peptide (POMC) on the N-ter…

   Promoter

   hSynapsin

   Available Since

   Dec. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC3.1_CMV_oROS-G

   Plasmid

   #216111

   Purpose

   Expresses the genetically encoded green fluorescent hydrogen peroxide sensor in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-G
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC3.1_CAG_oROS-Gr_LF(C199S)

   Plasmid

   #216114

   Purpose

   Expresses the loss-of-function version of the genetically encoded, ratiometric hydrogen peroxide sensor oROS-Gr in mamalian cells.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.01.31.578117

   Insert

   oROS-Gr_LF(C199S)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-AAV-syn-iGABASnFR2-WPRE

   Plasmid

   #218874

   Purpose

   AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

   Depositor

   GENIE Project

   Article

   Kolb et al bioRxiv 2025.03.25.644953

   Has Service

   AAV1 and AAV5

   Insert

   iGABASnFR2
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   S99A F102Y F104Y L178S

   Promoter

   Synapsin

   Available Since

   June 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

   Plasmid

   #223175

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #223173

   Purpose

   Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hfCas13d (N2V8)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Available Since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. ATP5F1B-turboGFP

   Plasmid

   #239795

   Purpose

   Expresses human ATP5F1B tagged with turboGFP

   Depositor

   Samantha Lewis

   Article

   Rose et al bioRxiv [Preprint]. 2025 Mar 25:2025.03.

   Insert

   ATP5F1b (ATP5F1B Human)
   

   Tags

   turboGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-REPORT(EF1a)-TTN

    Plasmid

    #172327

    Purpose

    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Inserts

    monomeric nuclear Tomato
    d2eGFP
    HygR
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3x SV40 NLS

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    June 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. HA-MBP-TEVpCS-ADGRC3 CTFdeltaTA-FLAG

    Plasmid

    #217719

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2522

    Available Since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC1_oROS-HT

    Plasmid

    #216413

    Purpose

    Expresses the genetically encoded, chemigenetic hydrogen peroxide sensor oROS-HT in mamalian cells.

    Depositor

    Andre Berndt

    Article

    Lee et al bioRxiv 2024.02.06.579232

    Insert

    oROS-HT
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. WT USP7 catalytic

    Plasmid

    #227201

    Purpose

    Expresses human USP7 Catalytic domain with a N-terminal cleavable 6His tag in E. coli

    Depositor

    Irina Bezsonova

    Article

    Valles et al bioRxiv [Preprint]. 2023 Jan 12:2023.01.

    Insert

    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    

    Tags

    6His

    Expression

    Bacterial

    Mutation

    aa 207-560

    Promoter

    T7-promoter

    Available Since

    Oct. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPolI_SARS-CoV-2_NLuc

    Plasmid

    #183953

    Purpose

    Encodes SARS-CoV-2 minigenome with nano-luciferase reporter gene. Minigenome is under control of the Pol I promoter

    Depositor

    AJ te Velthuis

    Article

    Vial et al bioRxiv 2021.06.28.450211

    Insert

    SARS-CoV-2 minigenome with Nano luciferase (N, ORF10 SARS coronavirus 2)
    

    Expression

    Mammalian

    Promoter

    Mammalian Pol I

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-MBP-TEVpCS-ADGRC3 CTF-FLAG

    Plasmid

    #217686

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    CELSR3 (CELSR3 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-2516

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. eGFP-SUN1

    Plasmid

    #213515

    Purpose

    Expresses human eGFP-SUN1 in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    SUN1 (SUN1 Human)
    

    Expression

    Mammalian

    Mutation

    SUN1 has a silent mutation on its gene (nucleotid…

    Promoter

    CMV

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. fabp10a:lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0; cmlc2:EGFP

    Plasmid

    #230075

    Purpose

    Partial ablation using NTR2 and subsequent lineage tracing of regenerating hepatocytes in zebrafish.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    lox2272-loxp-nls-mTagBFP2-stop-lox2272-H2B-mGL-stop-loxp-mCherry-NTR2.0
    

    Use

    Cre/Lox; Zebrafish expression

    Promoter

    fabp10a

    Available Since

    May 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-CAG-flex-iGABASnFR2-WPRE

    Plasmid

    #218880

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. Cas12aUltra_5XNLS

    Plasmid

    #218775

    Purpose

    His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

    Depositor

    Ross Wilson

    Article

    Sahu et al bioRxiv 2024.07.14.603391

    Insert

    5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
    

    Use

    CRISPR

    Tags

    6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

    Expression

    Bacterial

    Mutation

    Nucleotides mutations were made to encode for arg…

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_Gal4UASpyb-TATA_tBFP_pGK_mCitrine (RL407)

    Plasmid

    #194217

    Purpose

    Lentiviral transducition vector for a GAL4-responsive tagBFP reporter gene

    Depositor

    Hana El-Samad

    Article

    Weinberg et al bioRxiv 2021.04.20.440678

    Insert

    UAS-ybTATA-tagBFP; PGK-mCitrine
    

    Use

    Lentiviral

    Promoter

    GAL4 UAS-ybTATA; PGK

    Available Since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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