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We narrowed to 4,953 results for: codon optimized

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Showing: 3061 - 3080 of 4953 results

  1. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Publication
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  2. SpCas9-NRCH-ABE8.17-m+V106W

    Plasmid
    #198551
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NRCH-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-ABE8.17-m+V106W
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198551-plasmid-map-sequence-id-388896

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  3. SpCas9-NRCH-APOBEC-nCas9-Ung

    Plasmid
    #198552
    Purpose
    Expresses human codon-optimized SpCas9-NRCH-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NRCH-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NRCH-APOBEC-nCas9-Ung
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198552-plasmid-map-sequence-id-388895

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  4. SpCas9-NG-ABE8.17-m+V106W

    Plasmid
    #198534
    Purpose
    Expresses human codon-optimized SpCas9-NG-ABE8.17-m+V106W and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8.17-m+V106W-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-ABE8.17-m+V106W
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198534-plasmid-map-sequence-id-388875

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  5. SpCas9-NG-miniCGBE1

    Plasmid
    #198538
    Purpose
    Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-miniCGBE1
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198538-plasmid-map-sequence-id-388888

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  11. CoV2-N-P13L

    Plasmid
    #177946
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P13L). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P13L) (N )
    Expression
    Mammalian
    Mutation
    P13L
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177946-plasmid-map-sequence-id-348197

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  12. CoV2-N-P80R

    Plasmid
    #177947
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (P80R) (N )
    Expression
    Mammalian
    Mutation
    P80R
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177947-plasmid-map-sequence-id-348196

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  13. CoV2-N-S235F

    Plasmid
    #177956
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (S235F). Used for generating RNA packaging virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Nucleocapsid (S235F) (N )
    Expression
    Mammalian
    Mutation
    S235F
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    177956-plasmid-map-sequence-id-348185

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  14. B-SpCas9

    Plasmid
    #126760
    Purpose
    Expression plasmid for human codon-optimized Blackjack-SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of WT SpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    amino acids 1005-1013 replaced with two glycine
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126760-plasmid-map-sequence-id-261519

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  15. B-eSpCas9

    Plasmid
    #126761
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    B-eSpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    K848A; K1003A; R1060A; amino acids 1005-1013 repl…
    Promoter
    CBh
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    126761-plasmid-map-sequence-id-259548

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  16. pBT3037

    Plasmid
    #122544
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian p62 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_sqst-1::CemOrange2 (T12G3.1 Nematode, Synthetic)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122544-plasmid-map-sequence-id-238132

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  17. pBT3043

    Plasmid
    #122546
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::lgg-1 (lgg-1 Nematode, Synthetic)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122546-plasmid-map-sequence-id-238140

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  18. pBT3102

    Plasmid
    #122548
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a plasma membrane localized gene (aqp-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_aqp-1::CemOrange2 (aqp-1 Nematode, Synthetic)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122548-plasmid-map-sequence-id-238198

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  19. pBT2999

    Plasmid
    #122535
    Purpose
    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a lysosome localized gene (lmp-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_lmp-1::CemOrange2 (lmp-1 Nematode, Synthetic)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122535-plasmid-map-sequence-id-238208

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Showing: 3061 - 3080 of 4953 results