Heterologous reporter expression in the planarian Schmidtea mediterranea through somatic mRNA transfection.
Hall RN, Weill U, Drees L, Leal-Ortiz S, Li H, Khariton M, Chai C, Xue Y, Rosental B, Quake SR, Sanchez Alvarado A, Melosh NA, Fire AZ, Rink JC, Wang B
Cell Rep Methods. 2022 Sep 20;2(10):100298. doi: 10.1016/j.crmeth.2022.100298. eCollection 2022 Oct 24. PubMed Article
Hall RN, Weill U, Drees L, Leal-Ortiz S, Li H, Khariton M, Chai C, Xue Y, Rosental B, Quake SR, Sanchez Alvarado A, Melosh NA, Fire AZ, Rink JC, Wang B
Cell Rep Methods. 2022 Sep 20;2(10):100298. doi: 10.1016/j.crmeth.2022.100298. eCollection 2022 Oct 24. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 186753 | pNHT7 | A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly. |
| 186754 | pNHT7::YB1::sNluc1 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1, CAI = 0.925) with y-box binding protein 5' and 3' UTRs. |
| 186755 | pNHT7::ENO::sNluc1 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with enolase 5' and 3' UTRs. |
| 186756 | pNHT7::RPL10::sNluc1 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL10 5' and 3' UTRs. |
| 186757 | pNHT7::RPL15::sNluc1 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc1) with RPL15 5' and 3' UTRs. |
| 186758 | pNHT7::YB1::sNluc0 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0, CAI = 1.0) with y-box binding protein 5' and 3' UTRs. |
| 186759 | pNHT7::YB1::sNluc2 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2, CAI =0.713) with y-box binding protein 5' and 3' UTRs. |
| 186760 | pNHT7::YB1::sNluc3 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc3, CAI = 0.663) with y-box binding protein 5' and 3' UTRs. |
| 186761 | pNHT7::YB1::sNluc4 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc4, CAI = 0.743) with y-box binding protein 5' and 3' UTRs. |
| 186762 | pNHT7::YB1::sNluc5 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc5, CAI = 0.633) with y-box binding protein 5' and 3' UTRs. |
| 186763 | pNHT7::RPL15::sNluc2 | An improved mRNA product vector encoding a codon-optimized nanoluciferase (sNluc2) with RPL15 5' and 3' UTRs. |
| 186764 | pGAPDH::H2B-mScarlet | A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter. |
| 186765 | pNHT7::UMSBP::sNluc2_ORF1 | An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at the endogenous gene's start codon. |
| 186766 | pNHT7::UMSBP::sNluc2_ORF2 | An mRNA production vector encoding planarian codon-optimized sNluc2 with UMSBP 5' and 3' UTRs beginning at a start codon further downstream of the endogenous gene's start codon.. |
| 186767 | pNHT7::RPL15::sNluc2_STOP | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) containing a premature stop codon. |
| 186768 | pNHT7::RPL15::hGluc | An mRNA production vector encoding human codon-optimized gaussia luciferase (hGluc) with RPL15 5' and 3' UTRs. |
| 186769 | pNHT7::RPL15::sGluc | An mRNA production vector encoding planarian codon-optimized gaussia luciferase (sGluc) with RPL15 5' and 3' UTRs. |
| 186770 | pNHT7::ENO::sNluc2 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc2) with enolase 5' and 3' UTRs |
| 186771 | pNHT7::RPL15::sNluc0 | An mRNA production vector encoding planarian codon-optimized nanoluciferase (sNluc0) with RPL15 5' and 3' UTRs |
| 186772 | pNHT7::RPL15::hNluc | An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with RPL15 5' and 3' UTRs |
| 186774 | pNHT7::YB1::hNluc | An mRNA production vector encoding human codon-optimized nanoluciferase (hNluc) with y-box binding protein 5' and 3' UTRs |
| 187221 | pGAPDH::sNluc1 | A plasmid containing a planarian codon-optimized Nluc (sNluc1), driven by the planarian GAPDH promoter. |