We narrowed to 8,845 results for: plat

1. CLYBL-TO-hNGN2-BSD-mApple

   Plasmid

   #124229

   Purpose

   Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.

   Depositor

   Michael Ward

   Article

   Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

   Insert

   NGN2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. Anti-GFP [N86/8R]

   Plasmid

   #177572

   Purpose

   Mammalian Expression Plasmid of anti-GFP (Aequorea victoria). Derived from hybridoma N86/8R.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Anti-TrpC1 [1F1R]

   Plasmid

   #177437

   Purpose

   Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Anti-GST [N100/13R ]

   Plasmid

   #188214

   Purpose

   Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Dual CMV

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

   Plasmid

   #223159

   Purpose

   Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1-GGamma5-GFP2

   Plasmid

   #166777

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma5-GFP2 (GNG5 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-GGamma4-GFP2

   Plasmid

   #166776

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma4-GFP2 (GNG4 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVD1 tRNA-gRNA position E2 (GB2239)

   Plasmid

   #160561

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

   Insert

   tRNA-gRNA position E2 (Multiplexing Edit)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-GGamma10-GFP2

   Plasmid

   #166779

   Purpose

   Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma10-GFP2 (GNG10 Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains an N-terminal GFP2 and GSAG linker

   Promoter

   CMV

   Available since

   April 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Anti-GFP [N86/34R]

    Plasmid

    #220419

    Purpose

    Mammalian Expression Plasmid of anti-GFP (Jellyfish) IgG2a R-mAb. Derived from hybridoma N86/34

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Anti-GFP (Aequorea victoria) recombinant mouse monoclonal antibody.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Bxb1-GA_AIO-GZV-SynZiFTR_mNgn2 donor

    Plasmid

    #229789

    Purpose

    Bxb1-GA donor plasmid with downstream tandem syntax for all-in-one grazoprevir-inducible expression of mouse neurogenin 2

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mNgn2; ZF10-NS3-p65 (Neurog2 Mouse)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF10 and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. ITGB1

    Plasmid

    #51920

    Purpose

    Expresses full-length Integrin beta-1 precursor ectodomain in mammalian cells. Stop codon before C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    ITGB1 (ITGB1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Stop Codon at amino acid 728, before CD4, bio and…

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-SIN-BX SpyCatcher003-(short)mCD80

    Plasmid

    #210567

    Purpose

    Expresses Spycatcher003 on the cell surface using mouse CD80 (short) hinge, transmembrane, and cytoplasmic domain

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Spycatcher003 fused to mouse CD80 (short hinge + transmembrane + cytoplasmic domain)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    Dec. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIG-747pUC19-tNGFR-P2A-CD7

    Plasmid

    #186086

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CD7 (CD7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Pan-QKI scFv [N147/6]

    Plasmid

    #211605

    Purpose

    Mammalian Expression Plasmid of Pan-QKI scFv. Derived from hybridoma N147/6.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Pan-QKI (Homo sapiens) recombinant mouse scFv. (QKI Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Aldh1L1 scFv [N103/31]

    Plasmid

    #190522

    Purpose

    Mammalian Expression of Aldh1L1 scFV. Derived from hybridoma N103/31.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    Aldh1L1 (Rattus norvegicus) recombinant scFV (Aldh1l1 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS

    Plasmid

    #115694

    Purpose

    All-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.

    Depositor

    Erik Sontheimer

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Inserts

    NmeCas9
    Nme-sgRNA
    

    Use

    CRISPR

    Tags

    NLS and NLS, HA

    Expression

    Insect and Mammalian

    Mutation

    None and fusion of repeat/tracrRNA to make a sgRNA

    Promoter

    EF-1 alpha and U6

    Available since

    Sept. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. VGlut1 scFv [N28/9]

    Plasmid

    #182084

    Purpose

    Mammalian Expression of VGlut1 scFv. Derived from hybridoma N28/9.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting VGlut1 (Rattus norvegicus) (Slc17a7 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-mCherry-DMDEx23-eGFP

    Plasmid

    #211366

    Purpose

    Piggybac transposon plasmid for a DMD exon 23 skipping reporter (mCherry-DMDEx23)

    Depositor

    Ulrich Lächelt

    Article

    Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

    Insert

    mCherry-DMDEx23-eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mCherry interrupted by mdx dystrophin exon 23 bet…

    Promoter

    CAG

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO-GAlpha15-RLuc8

    Plasmid

    #140984

    Purpose

    Encodes a G alpha subunit (GNA15) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlpha15-RLuc8 (GNA15 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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