We narrowed to 4,734 results for: Drosophila

1. pET28a_DmKHC[1-421]-SNAP-6xHis

   Plasmid

   #196975

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   6xHis and SNAP

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHD-DsRed

   Plasmid

   #51434

   Purpose

   Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed.

   Depositor

   Kate O'Connor-Giles

   Article

   Gratz et al Genetics. 2014 Apr;196(4):961-71. doi: 1

   Insert

   LoxP-3xP3-DsRed-LoxP
   

   Promoter

   3xP3

   Available Since

   Feb. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pT7-EGFP-C1-HsNXF-1_K

   Plasmid

   #146740

   Purpose

   Mammalian Expression of HsNxf-1

   Depositor

   Elisa Izaurralde

   Article

   Gatfield et al EMBO J. 2003 Aug 1. 22(15):3960-70.

   Insert

   HsNxf-1 (NXF1 Human)
   

   Expression

   Mammalian

   Mutation

   S144G relative to NM_006362.4

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP

   Plasmid

   #69908

   Purpose

   Expresses a GFP circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1, GFP (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1(+) ZKSCAN1 MCS Exon Vector

   Plasmid

   #69901

   Purpose

   Expression plasmid for expressing circular RNAs of a desired sequence in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ZKSCAN1 (ZKSCAN1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

   Plasmid

   #63166

   Purpose

   GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-HA"
   

   Tags

   N-myristoylation

   Expression

   Insect

   Available Since

   May 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

   Plasmid

   #71805

   Purpose

   Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
   

   Expression

   Insect

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSEED-LoxP-sfGFP:ALFA

   Plasmid

   #219660

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   sfGFP:ALFA SEED Cassette (LoxP)
   

   Available Since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. optoAKT

   Plasmid

   #207164

   Purpose

   Light-inducible expression of optoAKT; for precise temporal control of the AKT signaling pathway

   Depositor

   Kai Zhang

   Article

   Wang et al Elife. 2020 Oct 6;9:e57395. doi: 10.7554

   Insert

   CRY2-mCherry-AKT(_PH)-2A-CIBN2-GFP-CAAX
   

   Expression

   Mammalian

   Available Since

   Nov. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPaint-T2A-Gal4-3xP3-RFP

    Plasmid

    #127556

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    Gal4
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF3BGX-7xGFP11

    Plasmid

    #138397

    Purpose

    RMCE vector for generating C-terminal fusion of seven tandem GFP11 tags (split GFP)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    7xGFP11
    

    Expression

    Insect

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBPGAL4.2::p65Uw

    Plasmid

    #26229

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4::p65 (RELA Budding Yeast, Human)
    

    Expression

    Insect

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Promoter

    GAL4-responsive UAS hsp70

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pF3BGX-T2A-p65-AD

    Plasmid

    #138395

    Purpose

    RMCE vector for generating C-terminal T2A-p65-AD fusion (split GAL4)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-p65-AD
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC-U63-QtgRNA2.1-BR

    Plasmid

    #170513

    Purpose

    gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pF3BGX-T2A-GAL4-DBD

    Plasmid

    #138394

    Purpose

    RMCE vector for generating C-terminal T2A-GAL4-DBD fusion (split GAL4)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL4-DBD
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

    Plasmid

    #63167

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-cMyc"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMT-Hrp48-ADARcd-E488Q-V5

    Plasmid

    #139685

    Purpose

    Expresses the hyperactive form of the catalytic domain of the ADAR protein fused to HRP-48 for use in "TRIBE" assays

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Hrp48, ADAR (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. hMTP

    Plasmid

    #124281

    Purpose

    Expresses human MTP in mammalian cells.

    Depositor

    Mahmood Hussain

    Article

    Sellers et al J Biol Chem. 2003 May 30;278(22):20367-7

    Insert

    MTTP (MTTP Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ910

    Plasmid

    #44481

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, NeoR, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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