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pYTK001_O2Aq(1st)

Plasmid

#190917

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Ab

Plasmid

#183083

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Ab

Plasmid

#183084

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_E2Aq(1st)

Plasmid

#183088

Purpose

2A peptide E2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

E2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Aq(2nd)

Plasmid

#183089

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Publication

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pRS42K_PCP-VP64_A-PP7

Plasmid

#177156

Purpose

Expresses transactivator (PCP-VP64) and scRNA (A-PP7) in yeast cells

Depositor

Gautam Dantas

Publication

Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

Inserts

PCP-VP64
A-PP7

Tags

SV40 NLS

Expression

Yeast

Promoter

ADH1 promoter and SNR52 promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC530

Plasmid

#135434

Purpose

Yeast integrating plasmid for pTETO1-Venus reporter gene

Depositor

Jesse Zalatan

Publication

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

pTETO1_Venus

Expression

Yeast

Promoter

pTETO1

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJS032

Plasmid

#135431

Purpose

Yeast integrating plasmid to express PYL1-VP64

Depositor

Jesse Zalatan

Publication

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

PYL1-VP64

Expression

Yeast

Promoter

pAdh

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJS033

Plasmid

#135432

Purpose

Yeast integrating plasmid to express MCP-ABI

Depositor

Jesse Zalatan

Publication

Cunningham-Bryant et al Chembiochem. 2019 Jun 14;20(12):1519-152

Insert

MCP-ABI

Expression

Yeast

Promoter

pTEF1

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymVenus-T2A-mTurquoise2

Plasmid

#118443

Purpose

Produces equimolar expression of yeast codon-optimized mVenus and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymVenus-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yotagRFPT-T2A-mTurquoise2

Plasmid

#118449

Purpose

Produces equimolar expression of yeast codon-optimized tagRFPT and mTq2

Depositor

Bas Teusink

Publication

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yotagRFPT-T2A-mTurquoise2

Expression

Yeast

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20Tpm2

Plasmid

#99356

Purpose

Bacterial expression of budding yeast (S. cerevisiae) Tropomyosin, Tpm2

Depositor

Dan Mulvihill

Publication

Skoumpla et al J Cell Sci. 2007 May 1;120(Pt 9):1635-45

Insert

Tropomyosin (TPM2 Budding Yeast)

Expression

Bacterial

Promoter

T7

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRS413 Prp5 AAAA

Plasmid

#87600

Purpose

Encodes Prp5 AAAA for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

D136A, P137A, L138A, D139A

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 E235A

Plasmid

#87601

Purpose

Encodes Prp5 E235A for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

E235A

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 N399D

Plasmid

#87602

Purpose

Encodes Prp5 N399D for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

N399D

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS413 Prp5 TAG

Plasmid

#87603

Purpose

Encodes Prp5 SAT motif to TAG for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

PRP5 (PRP5 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

S446T, T448G

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 P369E

Plasmid

#87277

Purpose

Encodes Hsh155 P369E for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

P369E

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 K335E

Plasmid

#87273

Purpose

Encodes Hsh155 K335E for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

K335E

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294L

Plasmid

#87269

Purpose

Encodes Hsh155 R294L for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294L

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRS414 Hsh155 R294C

Plasmid

#87268

Purpose

Encodes Hsh155 R294C for expression in yeast

Depositor

Aaron Hoskins

Publication

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

R294C

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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