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p415 TEF roGFP2‐Tsa2ΔCR

Plasmid

#83238

Purpose

The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCr

Tags

roGFP2 fusion with Tsa2dCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 stuffer v3

Plasmid

#158046

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA casette (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pJH2685

Plasmid

#191372

Purpose

Punc-4-TeTx::mCherry unc-54 3' UTR C.elegans A MNs/others neurou expression of TeTx RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Tetanus Toxin Light Chain

Tags

wCherry

Expression

Worm

Promoter

Punc-4

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Cre stuffer v4

Plasmid

#158032

Purpose

lenti-viral construct with Cre recombinase and U6 driven sgRNA casette with modified tracrRNA (20bp sgRNA can be cloned by digesting the stuffer 1.8kb with BsmBI). NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pAM-AAV-mSncg-Cre

Plasmid

#153207

Purpose

Mouse gamma-synuclein (mSncg) promoter-mediates Cre expression in retinal ganglion cells

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Insert

Cre

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

mSncg

Available Since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p-PDI

Plasmid

#202486

Purpose

Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPc3 N-RAS

Plasmid

#236450

Purpose

transient overexpression of N-RAS in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

N-RAS (NRAS Human)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-rab7 DN

Plasmid

#12660

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB7 (Homo sapiens) (RAB7A Human)

Tags

GFP and HA

Expression

Mammalian

Mutation

T22N

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

Plasmid

#206461

Purpose

Oskar 139-240 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAM-AAV-mSncg-0.27kb-EGFP

Plasmid

#153185

Purpose

Truncated mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates gene expression in retinal ganglion cells with fluorescent reporter

Depositor

Yang Hu

Article

Wang et al J Neurosci. 2020 Apr 9. pii: JNEUROSCI.0

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F3B

Plasmid

#236438

Purpose

transient overexpression of E2F3B in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3B (E2F3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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TIRAP-GFP

Plasmid

#52739

Purpose

Retroviral vector expresses WT hTIRAPfused to GFP. Also contains IRES-hCD2 as selectable marker

Depositor

Jonathan Kagan

Article

Bonham et al Cell. 2014 Feb 13;156(4):705-16. doi: 10

Insert

Toll/IL-1 Receptor adaptor protein (TIRAP Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pET28a His6HuR

Plasmid

#135943

Purpose

Expresses HuR in E. coli

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA E2F3A

Plasmid

#236437

Purpose

transient overexpression of E2F3A in mammalian cells

Depositor

Michele Pagano

Article

Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

Insert

E2F3A (E2F3 Human)

Tags

HA

Expression

Mammalian

Available Since

May 28, 2025

Availability

Academic Institutions and Nonprofits only

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pC5Kan-P2A

Plasmid

#51814

Purpose

P2A peptide from porcine teschovirus-1 flanked by restriction sites in shuttle vector for easy cloning of multiple separate proteins to be expressed from single message

Depositor

Barry Ganetzky

Article

Daniels et al PLoS One. 2014 Jun 19;9(6):e100637. doi:

Insert

P2A peptide (PTV1gp1 Synthetic, porcine teschovirus-1)

Use

Shuttle vector for insect expression vectors

Mutation

codons altered for Drosophila and to eliminate re…

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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DsRed-Rab5 DN

Plasmid

#13051

Depositor

Richard Pagano

Article

Sharma et al J Biol Chem. 2003 Feb 28. 278(9):7564-72

Insert

RAB5A (Canis familiaris) (RAB5A Canis familiaris)

Tags

DsRed2 and HA

Expression

Mammalian

Mutation

asparagine 3 changed to serine (neutral); aspara…

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pACE-GIRK2-S

Plasmid

#172426

Purpose

Expression of full-length, human GIRK2 with C-terminal StrepTag II

Depositor

Arthur Laganowsky

Article

Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

Insert

GIRK2 (KCNJ6 Human)

Tags

Streptag II

Expression

Insect

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pJH3717

Plasmid

#191477

Purpose

Pacr-2s-Arch::wCherry unc-54 3' UTR C.elegans A/B MN expression of Arch RFP

Depositor

Mei Zhen

Article

Lu et al Curr Biol. 2022 Nov 7;32(21):4631-4644.e

Insert

Arch (arch-1 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pacr-2s

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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T7-Elongin C-pcDNA3

Plasmid

#19998

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

Elongin C (ELOC Human)

Tags

T7-tag

Expression

Mammalian

Available Since

April 6, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Cyclin F

Plasmid

#236466

Purpose

transient overexpression of Cyclin F in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

Cyclin F (CCNF Human)

Tags

HA

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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