We narrowed to 8,230 results for: 11
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Plasmid#222354PurposeTn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneExpressionBacterialAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only
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pCEP4-myc-EphB1
Plasmid#200981PurposeMammalian expression plasmid for myc-tagged EphB1DepositorInsertEphB1 (EPHB1 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-EphB6
Plasmid#200984PurposeMammalian expression plasmid for myc-tagged EphB6DepositorInsertEphB6 (EPHB6 Human)
TagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianPromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EphB3-Fc
Plasmid#200985PurposeMammalian expression plasmid for soluble EphB3 fused to IgG1 FcDepositorInsertEphB3 (EPHB3 Human)
TagsFc region of human IgG1ExpressionMammalianMutationSoluble ectodomain of EphB3PromoterCMVAvailable sinceOct. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-88)-Strep
Plasmid#196214PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-88)-Strep.DepositorAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-A11(2-196, D40G)-Strep
Plasmid#196210PurposeCodon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, D40G)-Strep.DepositorInsertAnnexin A11 (ANXA11 Human)
TagsGB1-6xHis-TEV and Strep-II tagExpressionBacterialMutationD40GAvailable sinceJuly 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS791
Plasmid#188889PurposeTargeting vector for insertion into 5' miniMos Arm; carries floxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFPDepositorInsertfloxed Cbr-unc-119 marker, Phsp16.41::Cre and Pmyo-2::2xNLS::cyOFP
ExpressionWormMutationC. elegans codom optimization and intron additionAvailable sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pODN-mR2-ACTB
Plasmid#183868PurposeRepair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.DepositorInsertACTB homology arms with mRuby2-linker (ACTB Human)
UseCRISPR; Donor templateTagsmRuby2-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSCV-HA-Nr4a1-AA
Plasmid#160942PurposeGeneration of retrovirus for the overexpression of Nr4a1 mutant (287C 288EAA)DepositorInsertNr4a1 (Nr4a1 Mouse)
UseRetroviralTagsHA tagExpressionMammalianMutationChange Cys 287 Glu 288 into Ala AlaPromoterMSCV-LTRAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-T3-CjCAS9-pA
Plasmid#131543PurposeExpresses Campylobacter jejuni-derived Cas9 nuclease in mammalian cells. Used to modify genome of mouse embroysDepositorInsertCjCas9
UseCRISPRTagsFLAG-SV40NLS, SV40NLS, and polyAExpressionMammalianMutationHuman codon-optimizedPromoterCAG, T3Available sinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsubDD-YPet-NES
Plasmid#84633PurposeEncodes C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsubDD-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianPromoterCMVAvailable sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pONSY-Venus
Plasmid#111875PurposeThis plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy.DepositorInsertVenus
UseCapsaspora owczarzakiTagsVenusPromoterElongation Factor 1 alpha (EF1a) from CapsasporaAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pconst #1-RFP
Plasmid#81075Purposeconstitutive promoter expressing RFP, biobrick constructionDepositorInsertRFP
UseP15a origin, constitutive promoter, kanrPromoterConstitutive Promoter - BBa_J23119Available sinceAug. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Cx30
Plasmid#69022PurposeExpresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorAvailable sinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
Gal4pBS4-Pleum
Plasmid#60329PurposeContains a promoter of a gal binding site coupled with a core promoter derived from native Leucine promoter.DepositorInsertpromoter
UseSynthetic Biology; Yeast synthetic hybrid promote…ExpressionYeastAvailable sinceFeb. 11, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
TALEN-CCR5L18-NtermN1
Plasmid#51444PurposeExpresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domainDepositorInsertTALEN CCR5 Left (L18) N1 N-term
UseTALENTags3xFLAG and Fok1 EL variantExpressionMammalianMutationK150QPromoterCMVAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPROBE-AT
Plasmid#37812DepositorInsertPromotorless gfp reporter gene
Available sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α1.1-ChrimsonR-tdTomato
Plasmid#122064PurposeAAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.DepositorInsertChrimsonR-tdTomato
UseAAVTagstdTomato (codon diversified version)ExpressionMammalianMutationChrimson K176R mutantPromoterEF1a(1.1kb short version)Available sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3
Plasmid#106206PurposeWeak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertSF-iGluSnFR.S72A
UseAAVMutationGltI: S72A + mRuby3 fusionPromoterCAGAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only