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pcDNA-Flag1-3HABeta2-mEos2

Plasmid

#114187

Purpose

studying clustering of Beta2 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Publication

Annibale et al Nat Methods. 2011 Jun 12;8(7):527-8. doi

Insert

Adrenergic receptor beta 2 (ADRB2 Human)

Tags

3HA, FLAG, and mEOS2

Expression

Mammalian

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCVA-amiR-PDS

Plasmid

#32911

Depositor

Yule Liu

Publication

Tang et al Plant Physiol. 2010 Jun;153(2):632-41. E

Insert

amiR-PDS (Nb)

Use

Plant expression

Available since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa-F

Plasmid

#101193

Purpose

A fast switching Dronpa variant

Depositor

Peter Dedecker

Publication

Duwe et al Chem Commun (Camb). 2017 Jun 29;53(53):7

Insert

ffDronpa-F

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Mutation

K45I, V60A, F173V

Promoter

T7

Available since

Sept. 20, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRL1063a

Plasmid

#70687

Purpose

pRL1058 with Vibrio fischeri luxAB for sensing

Depositor

Peter Wolk

Publication

Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

Insert

Transposon Tn5-1063

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP N1 Ub-CRT

Plasmid

#69619

Purpose

Express cytoplasmic mature CRT.

Depositor

Marta Hallak

Publication

Goitea et al J Biol Chem. 2015 Jun 26;290(26):16403-1

Insert

Ubiquitina-Calreticulin (mature)

Tags

EGFP and Ubiquitin

Expression

Mammalian

Mutation

Deletion of signal peptide of calreticulin at the…

Promoter

CMV

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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C-Ag20(8)

Plasmid

#11867

Depositor

Martin Smith

Publication

Hoover et al J Cell Biol. 2003 Jun 9. 161(5):923-32.

Insert

Agrn (Agrn Mouse)

Tags

His and myc

Expression

Bacterial

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only
pGLOW30

Plasmid

#172697

Purpose

C. elegans mScarlet co-injection marker

Depositor

Ryan Doonan

Publication

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mScarlet

Tags

mScarlet

Expression

Worm

Promoter

myo-2

Available since

Sept. 2, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pyc111f

Plasmid

#158780

Purpose

ribosome-tagged, Cre-dependent GCaMP for soma localization

Depositor

Jennifer Garrison, Zachary Knight

Publication

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

GCaMP6f-RPL10a

Use

AAV

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pOD2046-bwmDEG

Plasmid

#89367

Purpose

MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmyo-3 (body wall muscle specific).

Depositor

Karen Oegema

Publication

Wang et al Development. 2017 Jun 15. pii: dev.15009

Insert

vhhGFP4-ZIF-1 (zif-1 Nematode, Synthetic)

Use

Targeting vector for mos1 transposon mediated sin…

Tags

vhhGFP4 (GFP nanobody)

Promoter

Pmyo-3

Available since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pUB_smFLAG_ActB_MS2

Plasmid

#81083

Purpose

Translation reporter: Encodes Beta-actin (374 aa) fused to smFLAG (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG).

Depositor

Tim Stasevich

Publication

Morisaki et al Science. 2016 Jun 17;352(6292):1425-9. d

Insert

smFLAG_ActB_24xMS2

Expression

Mammalian

Available since

Aug. 31, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-opto-mCasp1

Plasmid

#208781

Purpose

Inducibly expresses mouse caspase1 fused to light activatable Cry2

Depositor

Petr Broz

Publication

Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

mCherry mCaspase1 Cry2 (Casp1 Mouse)

Use

Lentiviral

Tags

mCherry, Cry2

Expression

Mammalian

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-CaM

Plasmid

#193816

Purpose

CMV overexpression of ChemoG-CaM in mammalian cell lines

Depositor

Julien Hiblot

Publication

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Expression

Mammalian

Mutation

EGFP:E116D, A206K; HT7: L271E

Promoter

CMV

Available since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCJH019_SC101_pLAC_Cas12c(4)_Kan

Plasmid

#183071

Purpose

Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

Depositor

Jennifer Doudna

Publication

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c_4

Expression

Bacterial

Mutation

Wild-type

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-MMLV RT

Plasmid

#178797

Purpose

Inducible expression of MMLV RT in E. coli

Depositor

Andrew Ellington

Publication

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

MMLV RT

Expression

Bacterial

Available since

March 7, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mas-GRK2RH-Nluc

Plasmid

#158605

Purpose

Plasmid expressing GRK2(RH)-Nluc in mammalian cells

Depositor

Mikel Garcia-Marcos

Publication

Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

Insert

myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker (GIKLGG) - GRK2 RH domain - linker (GGTGGS) - Nluc

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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mcherry-Myo 9b

Plasmid

#134918

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Publication

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shIFNAR

Plasmid

#127699

Purpose

Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

Depositor

Sandra Demaria

Publication

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shIFNAR1 Mmu (Ifnar1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Venus rGBD

Plasmid

#99015

Purpose

Used as a RhoA-GTP sensor

Depositor

Narasimhan Gautam

Publication

O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

Insert

rGBD (Rtkn Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C)

Plasmid

#79045

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Publication

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C)

Expression

Bacterial

Mutation

A14C and E45C mutations in HIV-1 CA protein

Promoter

T7

Available since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-XL4 ASXL1 (p.Y591X) 3x FLAG

Plasmid

#74261

Purpose

Cancer-associated ASXL1 truncation mutant in mammalian expression vector

Depositor

Anjana Rao

Publication

Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

Insert

ASXL1 (ASXL1 Human)

Tags

3X FLAG

Expression

Mammalian

Mutation

p.Y591X truncation

Promoter

CMV

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article