We narrowed to 14,886 results for: pET

1. pET-6xHis-GFP(-7)-neg_tag1

   Plasmid

   #219419

   Purpose

   Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

   Depositor

   Allie Obermeyer

   Article

   Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

   Insert

   GFP(-7)-neg_tag1
   

   Use

   Tags

   DEEEDD and MGHHHHHGG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-hfYFP-TEV-Bcx

   Plasmid

   #191195

   Purpose

   Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Bcx fusion
   

   Use

   Tags

   6xHis and Bacillus circulans xylanase

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-hfYFP-TEV-mScarlet-i

   Plasmid

   #191196

   Purpose

   Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-hfYFP-TEV-SAV

   Plasmid

   #191197

   Purpose

   Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   hfYFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-LSSmGFP-TEV-mScarlet-i

   Plasmid

   #191199

   Purpose

   Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & mScarlet-I fusion
   

   Use

   Tags

   6xHis and mScarlet-I

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-LSSmGFP-TEV-SAV

   Plasmid

   #191200

   Purpose

   Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

   Depositor

   Gregory Petsko

   Article

   Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

   Insert

   LSSmGFP & Streptavidin fusion
   

   Use

   Tags

   6xHis and Streptavidin core domain, amino acids 1…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-T5-MfnG-TEV-His

   Plasmid

   #191114

   Purpose

   Expresses MfnG-TEV-His in E. coli

   Depositor

   Han Xiao

   Article

   Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

   Insert

   MfnG
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28.His.3C.86b.8D2Q.Tau2N4R

   Plasmid

   #226400

   Purpose

   Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteria

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau (MAPT synthetic, Human)
   

   Use

   Tags

   His.3C.86b

   Expression

   Bacterial

   Mutation

   8D2Q.Tau2N4R

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28.His.3C.86b.20D3Q.Tau2N4R

   Plasmid

   #226401

   Purpose

   Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria

   Depositor

   Bradley Hyman

   Article

   Dickson et al J Neuropathol Exp Neurol. 2025 Jun 1;84(

   Insert

   Tau2N4R (MAPT synthetic, Human)
   

   Use

   Tags

   His.3C.86b

   Expression

   Bacterial

   Mutation

   20D3Q, Tau2N4R

   Promoter

   Available since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-PKP3(305-797)

    Plasmid

    #202599

    Purpose

    Expresses ARM domain of plakophilin3

    Depositor

    Tina Izard

    Article

    Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

    Insert

    plakophilin3 (PKP3 Human)
    

    Use

    Tags

    His8 tag with precision protease cleavage site

    Expression

    Bacterial

    Mutation

    Deleted amino acids 1 to 304

    Promoter

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

    Plasmid

    #187708

    Purpose

    Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 381-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-380

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a(+)-NEDD4L_W3-W4-HECT (492)

    Plasmid

    #187709

    Purpose

    Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

    Depositor

    Sandra Gabelli

    Article

    Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

    Insert

    NEDD4L residues 492-975 (NEDD4L Human)
    

    Use

    Tags

    His-HRV 3C site

    Expression

    Bacterial

    Mutation

    deletion of residues 1-491

    Promoter

    Available since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28-a(+)-6xHis-miRFP(-5)-HO1

    Plasmid

    #219424

    Purpose

    Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

    Depositor

    Allie Obermeyer

    Article

    Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

    Inserts

    miRFP682
    HO1
    

    Use

    Tags

    MGHHHHHGG

    Expression

    Bacterial

    Mutation

    Contains Ribosomal Binding Site for expression of…

    Promoter

    T7

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-NPR4 (SA binding domain)

    Plasmid

    #226885

    Purpose

    Expresses the Salicylic acid binding core (SBC) of NPR4 in E.coli, in an insoluble form

    Depositor

    Ning Zheng

    Article

    Wang et al Nature. 2020 Oct;586(7828):311-316. doi:

    Insert

    The Salicylic acid (SA) binding domain of NPR4 (NPR4 Mustard Weed)
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    NPR4 SA-binding core (residues 373-516), with a s…

    Promoter

    T7

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21a HsDHFR-bio-10His

    Plasmid

    #193883

    Purpose

    Expresses human dihydrofolate reductase (DHFR) with C-terminal AviTag (Bio) and His10 sequence

    Depositor

    James Inglese

    Article

    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

    Insert

    human dihydrofolate reductase (DHFR) (DHFR Human)
    

    Use

    Tags

    His tag and biotinylation sequence

    Expression

    Bacterial

    Mutation

    Codon optimized

    Promoter

    T7

    Available since

    Oct. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-UBE3A iso 2 N terminus

    Plasmid

    #185643

    Purpose

    expression of His-tagged UBE3A isoform 2 1-87 in bacterial cells

    Depositor

    Irina Bezsonova

    Insert

    ubiquitin-protein ligase E3A (UBE3A Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available since

    Sept. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-28b C7H1G6_6H N-terminal

    Plasmid

    #223434

    Purpose

    C7H1G6 GNAT expression vector with N-terminal His6 tag

    Depositor

    Emily Balskus

    Article

    Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

    Insert

    C7H1G6 GNAT
    

    Use

    Tags

    His6

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-28b R6CZ24_6H C-terminal

    Plasmid

    #223435

    Purpose

    R6CZ24 Thiolase expression vector with C-terminal His6 tag

    Depositor

    Emily Balskus

    Article

    Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

    Insert

    R6CZ24 Thiolase
    

    Use

    Tags

    His6

    Expression

    Mutation

    Promoter

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-28a (+)-Gal4-p53

    Plasmid

    #171076

    Purpose

    Expresses Gal4-p53 fusion protein in bacteria

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4 DBD (NEWENTRY Budding Yeast)
    

    Use

    Tags

    His and p53(1-85)

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-FKBP-IRSp53(pLxIS)

    Plasmid

    #221336

    Purpose

    Overexpression of IRSp53 pLxIS domain in bacteria

    Depositor

    Jonathan Kagan

    Article

    Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

    Insert

    IRSp53 pLxIS domain (Baiap2 Mouse)
    

    Use

    Tags

    6xHis-4xFKBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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