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Showing: 321 - 340 of 9101 results

  1. pEAT8-137 M1V

    Plasmid
    #173807
    Purpose
    Expression and purification of mature (no signal sequence) human alpha1-antitrypsin wildtype variant M1V (UniProt P01009) from E. coli BL21(DE3) cells
    Depositor
    Anne Gershenson
    Article
    Krishnan et al Methods Mol Biol. 2017;1639:195-209. doi
    Insert
    SERPiNA1 (SERPINA1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    E1M Otherwise this plasmid encodes the most commo…
    Promoter
    T7
    Available since
    Aug. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    173807-plasmid-map-sequence-id-341159

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  4. pBA2705

    Plasmid
    #189998
    Purpose
    Inducible expression of FBP75(1–200)-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for cytoplasmic proteins)
    Depositor
    Bungo Akiyoshi
    Article
    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    Expression
    Mutation
    N-terminal 600 bp of FBP75
    Promoter
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189998-plasmid-map-sequence-id-371593

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  5. pBA2675

    Plasmid
    #189997
    Purpose
    Inducible expression of FBP75 (1–200)-NLS-VhhGFP4, integrate at 177 bp, phleomycin marker (deGradFP for nuclear proteins)
    Depositor
    Bungo Akiyoshi
    Article
    Ishii et al Wellcome Open Res. 2022 Jun 23;7:175. do
    Inserts
    FBP75
    VhhGFP4
    Use
    Expression vector for trypanosoma brucei
    Tags
    NLS
    Expression
    Mutation
    N-terminal 600 bp of FBP75
    Promoter
    Available since
    Sept. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    189997-plasmid-map-sequence-id-371586

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  8. pLEW-pHluorin2-PTS1

    Plasmid
    #213776
    Purpose
    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei
    Depositor
    Kenneth Christensen
    Insert
    pHlorin2
    Use
    Trypanosoma brucei expression
    Tags
    Expression
    Mutation
    Appended a 2 glycine linker and a T. brucei perox…
    Promoter
    T. brucei rRNA promotor
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213776-plasmid-map-sequence-id-423197

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  9. pMSP1D12W/2Y

    Plasmid
    #124275
    Purpose
    Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence
    Depositor
    Alexey S. Ladokhin
    Insert
    Membrane scaffold protein
    Use
    Tags
    7xHis-tag
    Expression
    Bacterial
    Mutation
    Two tryptophans changed to two tyrosines
    Promoter
    T7
    Available since
    March 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    124275-plasmid-map-sequence-id-242402

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  11. pENTR/hBIG1

    Plasmid
    #226251
    Purpose
    Entry plasmid of hBIG1 for Gateway system
    Depositor
    Hye-Won Shin
    Insert
    BIG1 (ARFGEF1 Human)
    Use
    Entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226251-plasmid-map-sequence-id-454235

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  12. pENTR/HA-hBIG2

    Plasmid
    #226252
    Purpose
    Entry plasmid of N-terminally HA-tagged hBIG2 for Gateway system
    Depositor
    Hye-Won Shin
    Insert
    BIG2 (ARFGEF2 Human)
    Use
    Entry vector
    Tags
    HA
    Expression
    Mutation
    Promoter
    Available since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    226252-plasmid-map-sequence-id-454237

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  18. p5E-2.3k-fabp2

    Plasmid
    #84927
    Purpose
    –2.3kb promoter of zebrafish fabp2/ifabp gene in Gateway 5’ Entry vector
    Depositor
    John Rawls
    Insert
    –2.3kb promoter of zebrafish fabp2/ifabp gene (fabp2 Zebrafish)
    Use
    Tags
    NO
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    84927-plasmid-map-sequence-id-254043

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  20. pRSF-G1(6/7FTrp)RS

    Plasmid
    #207621
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    207621-plasmid-map-sequence-id-410952

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Showing: 321 - 340 of 9101 results