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pMV306G13+FFlucRT
Plasmid#49997PurposeIntegrating vector with an improved Red-shifted Thermostable Firefly LuciferaseDepositorInsertG13 promoter + Firefly luciferase Red Thermostable
UseMycobacteria integrating vectorTagsExpressionBacterialMutationCodon optimized for Mycobacterium tuberculosis wi…PromoterG13 from M. marinumAvailable sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAG-3xNLS-vsfGFP-0
Plasmid#191097PurposeTo express a bright green nanobody FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsert3xNLS-vsfGFP-0
UseAAVTagsGreen fluorescent protein bound to enhancer nanob…ExpressionMammalianMutationOptimized to Human codon usagePromoterCMV and CAGAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJGL002
Plasmid#180606PurposePlasmid expressing E. coli codon optimized His-MBP-TEV-PlmCasXDepositorInsertPlmCasX
UseCRISPRTags10xHis and MBPExpressionBacterialMutationPromoterT7Available sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGES201
Plasmid#190197PurposeFor CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selectionDepositorInsertspCas9
UseCRISPRTags3xFlagExpressionPlantMutationplant-codon optimizedPromoterGlycine max elongation factor 1A(pM4)Available sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro
Plasmid#42333PurposeThis plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.DepositorArticleInserthumanized S. pyogenes Cas9 (D10A) nickase
UseCRISPRTagsHAExpressionMammalianMutationD10A nickase-converting mutationPromoterAvailable sinceMarch 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET42b-ABE7.10
Plasmid#120398PurposeExpresses ABE7.10-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorInsertABE7.10
UseTagsHisExpressionBacterialMutationMammalian codon-optimizedPromoterT7Available sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExodus CMV.Trex2
Plasmid#40210PurposeMammalian expression of codon-optimized Trex2.DepositorInsertThree prime repair exonuclease 2 (Trex2 Mouse)
UseTagsExpressionBacterial and MammalianMutationPromoterCMV and T7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp15
Plasmid#165120Purposemammalian expression and localizationDepositorInsertNsp15 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cas12j-8-puro-crRNA
Plasmid#194965PurposeThe plasmid expresses human codon-optimized Cas12j-8, crRNA expression elements and puromycin resistence gene.DepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
TccCas13a Twin SUMO
Plasmid#199754PurposeExpresses codon optimized thermostabile TccCas13a in E.coliDepositorInsertTccCas13a
UseTagsSUMOExpressionBacterialMutationPromoterLacIAvailable sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pTH-iCre-WPREpA
Plasmid#213130PurposeTruncated rat TH promoter expressing iCreDepositorInsertiCre
UseAAV and Mouse TargetingTagsNoneExpressionMammalianMutationPromoterTruncated TH promoter from rat (Rattus norvegicus…Available sinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE
Plasmid#139094PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840APromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-5X-3-SARS-CoV-2-3CL
Plasmid#168457PurposeBacterial codon optimized expression vector for SARS-CoV-2 3CL protease.DepositorInsertSARS-CoV-2 3CL protease (ORF1ab Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
VPS13B^GFP
Plasmid#224588Purposecodon optimized GFP tagged VPS13B for expression in mammalian cellsDepositorInsertVPS13B^GFP (VPS13B Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX6p1-GST-Rnf31
Plasmid#63134PurposeExpression of human RNF31/HOIP codon optimized for E. coli and insect cellsDepositorInsertRnf31 (RNF31 Human, Synthetic)
UseTagsGSTExpressionBacterialMutationSynthetic based on human protein sequencePromotertacAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/er-(n2)oxStayGold(c4)
Plasmid#185822PurposeA soluble photostable fluorescent marker of the ER lumen. The StayGold gene has no codon optimization for mammalian expression.DepositorInserter-(n2)oxStayGold(c4)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR
Plasmid#104567PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activatorDepositorInserthuman codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
UseCRISPRTagsNLS-3xHA-VPRExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Nsp12
Plasmid#165117Purposemammalian expression and localizationDepositorInsertNsp12 (ORF1ab codon optimized SARS-COV-2)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHSN6A01
Plasmid#50586PurposeCRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistanceDepositorInsertsdCas9-VP64
gRNA scaffold
UseCRISPR; Plant expressionTags3xFLAG, HA, and NLSExpressionMutationdCas9 = defective in DNA cleavage; 4×minimal VP16…Promoter2×35Sp and AtU6-26pAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only
