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pJJBAD85

Plasmid

#15351

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CLN)

Tags

GB1

Expression

Bacterial

Mutation

F to L mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD86

Plasmid

#15352

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CCN)

Tags

GB1

Expression

Bacterial

Mutation

F to C mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD87

Plasmid

#15353

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CSN)

Tags

GB1

Expression

Bacterial

Mutation

F to S mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pJJBAD88

Plasmid

#15354

Depositor

Hideo Iwai

Article

Iwai et al FEBS Lett. 2006 Mar 20. 580(7):1853-8.

Insert

DnaE C-intein(CGN)

Tags

GB1

Expression

Bacterial

Mutation

F to G mutation

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only
pRSVBneoT

Plasmid

#22883

Depositor

James Pipas

Article

Srinivasan et al Mol Cell Biol. 1997 Aug . 17(8):4761-73.

Insert

Early region SV40

Expression

Mammalian

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

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pGEX4T moesin 1-577

Plasmid

#11637

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

moesin (MSN Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE-puro SV40 LT

Plasmid

#13970

Depositor

Thomas Roberts

Article

Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

Insert

SV40 LT (SV40gp6 )

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pMF230

Plasmid

#62546

Purpose

Broad host range plasmid for constitutive expression of the eGFP. Developed for imaging Pseudomonas aeruginosa in biofilms.

Depositor

Michael Franklin

Article

Nivens et al J Bacteriol. 2001 Feb;183(3):1047-57.

Insert

GFP mut2

Use

Broad host range plasmid

Promoter

trc

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-sfGFP

Plasmid

#85492

Purpose

pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

Insert

superfolder GFP

Tags

6x His

Expression

Bacterial

Promoter

T7

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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p1321 HPV-16 E6/E7

Plasmid

#8641

Depositor

Peter Howley

Article

Münger et al J Virol. 1989 Oct . 63(10):4417-21.

Insert

HPV-16 E6/E7 (E7, E6 Human papillomavirus)

Expression

Mammalian

Available Since

June 16, 2005

Availability

Academic Institutions and Nonprofits only

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pRK5_CKAR3

Plasmid

#238411

Purpose

Mammalian expression of CKAR3 under a CMV promotor.

Depositor

Haining Zhong

Article

Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

Insert

CKAR3 (PKC sensor)

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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RepX_pET28a(+)

Plasmid

#172199

Purpose

Expresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.

Depositor

Taekjip Ha

Article

Arslan et al Science. 2015 Apr 17;348(6232):344-7. do

Insert

RepX superhelicase

Tags

6xHis tag

Expression

Bacterial

Mutation

C18L, C43S, C167V, S400C, C612A

Available Since

March 16, 2022

Availability

Academic Institutions and Nonprofits only

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pMyBADC-kan

Plasmid

#84689

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

AraC

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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BoNT/A-LC

Plasmid

#31602

Depositor

Axel Brunger

Article

Zuniga et al Structure. 2008 Oct 8. 16(10):1588-97.

Insert

BoNT/A LC

Tags

His6

Expression

Bacterial

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pMyNT-kan

Plasmid

#84693

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Article

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Acetamidase

Available Since

Jan. 4, 2017

Availability

Academic Institutions and Nonprofits only

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Gamillus/ pcDNA3

Plasmid

#124837

Purpose

mammalian expression of Gamillus, an acid-tolerant monomeric green fluorescent protein

Depositor

Takeharu Nagai

Article

Shinoda et al Cell Chem Biol. 2018 Mar 15;25(3):330-33

Insert

Gamillus

Tags

No tag

Expression

Mammalian

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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4xM67 pTATA TK-Luc

Plasmid

#8688

Depositor

Jim Darnell

Article

Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

Insert

4 repeated M67 sites

Use

Luciferase

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pJYDNp

Plasmid

#162451

Purpose

Positive control plasmid carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid serves as an expression control.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-eUnaG2

Use

Shuttle vector bacteria/yeast

Tags

HA and myc

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-GFP(0)

Plasmid

#199166

Purpose

used to express a neutral GFP variant in E. coli

Depositor

Allie Obermeyer

Article

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(0)

Tags

MGHHHHHHGGA

Mutation

E16R, I138R, D207R, N222K, L246R

Promoter

T7

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSPTg.T2FpAXK (#52)

Plasmid

#35962

Depositor

Thomas Sato

Article

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Inserts

Tie2 promoter
SV40 pA
Tie2 enhancer

Available Since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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