Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 1,982 results for: rpe

Clear keywords
Showing: 341 - 360 of 1982 results

  1. Coexpressed Free GFP-NES

    Plasmid
    #182491
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    GFP-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182491-plasmid-map-sequence-id-360449

    Enlarge

  2. pXPR_220

    Plasmid
    #164560
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes (one constitutive, one inducible via Flp). Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Flp/frt
    Expression
    Mammalian
    Promoter
    Human U6 and Mouse U6
    Available Since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164560-plasmid-map-sequence-id-330240

    Enlarge

  3. Coexpressed Free NES

    Plasmid
    #182482
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182482-plasmid-map-sequence-id-360445

    Enlarge

  4. Coexpressed Free ClLIS1 (M)

    Plasmid
    #182484
    Purpose
    Yeast integrative plasmid for expressing ERG20(F96W-N127W) (GAL10 promoter) and limonene synthase from Citrus limon (ClLIS1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS(M)
    LS
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182484-plasmid-map-sequence-id-360440

    Enlarge

  5. Coexpressed Free deadFPPS-NES

    Plasmid
    #182492
    Purpose
    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein deadFPPS-AcNES1 (GAL7 promoter). deadFPPS is ERG20(K197G-K254A), an inactive mutant of ERG20.
    Depositor
    Claudia Vickers
    Article
    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.
    Inserts
    FPPS
    deadFPPS-NES
    Use
    Synthetic Biology; Metabolic engineering
    Expression
    Yeast
    Promoter
    GAL10 and GAL7
    Available Since
    Nov. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182492-plasmid-map-sequence-id-360446

    Enlarge

  6. pcDNA3.1-ccdB-V5

    Plasmid
    #214985
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal V5 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    V5
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214985-plasmid-map-sequence-id-440020

    Enlarge

  7. pcDNA3.1-ccdB-3xFLAG

    Plasmid
    #214984
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    3xFLAG
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214984-plasmid-map-sequence-id-440018

    Enlarge

  8. pcDNA3.1-ccdB-3xHA

    Plasmid
    #214986
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xHA tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    3xHA
    Expression
    Mammalian
    Available Since
    May 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214986-plasmid-map-sequence-id-440021

    Enlarge

  9. pcDNA3.1-ccdB-TwinStrep

    Plasmid
    #214988
    Purpose
    Gateway compatible empty vector (transient mammalian expression) with a C-terminal TwinStrep tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Tags
    TwinStrep
    Expression
    Mammalian
    Available Since
    March 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    214988-plasmid-map-sequence-id-440022

    Enlarge

  10. pHAGE-APEX2-nDEST

    Plasmid
    #214992
    Purpose
    Gateway compatible empty vector (lentiviral/mammalian expression) with an N-terminal APEX2 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Flag-APEX2
    Expression
    Mammalian
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    214992-plasmid-map-sequence-id-443329

    Enlarge

  11. pHAGE-cDEST-APEX2

    Plasmid
    #214993
    Purpose
    Gateway compatible empty vector (lentiviral/mammalian expression) with a C-terminal APEX2 tag
    Depositor
    Wade Harper
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    APEX2-Flag
    Expression
    Mammalian
    Available Since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    214993-plasmid-map-sequence-id-443330

    Enlarge

  15. pLenti UBC MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215010
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215010-plasmid-map-sequence-id-429275

    Enlarge

  16. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Available Since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215011-plasmid-map-sequence-id-429274

    Enlarge

  18. pDONR223 Rab25-STOP

    Plasmid
    #228034
    Purpose
    DONR vector for shuttling Rab25 into DEST vector. Contains a STOP codon.
    Depositor
    Wade Harper
    Insert
    Rab25 (RAB25 Human)
    Use
    Gateway donr
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228034-plasmid-map-sequence-id-450381

    Enlarge

  19. pHAGE mCherry-Rab25

    Plasmid
    #228039
    Purpose
    Mammalian lentiviral expression of mCherry-labeled Rab25.
    Depositor
    Wade Harper
    Insert
    Rab25 (RAB25 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Available Since
    Oct. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    228039-plasmid-map-sequence-id-450375

    Enlarge

Showing: 341 - 360 of 1982 results