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(Plasmid #179343)


Item Catalog # Description Quantity Price (USD)
Plasmid 179343 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Steve Elledge
  • Backbone size w/o insert (bp) 12095
  • Total vector size (bp) 13070
  • Modifications to backbone
    insertion of UL148-HA by AgeI-MluI. Note: pOUPc is an extensively modified version of pInducer20 (Elledge lab shRNA vector) that is used to express proteins. The CMV IE2 binding site (crs) in the TRE 3G promoter is also mutated so that it would not be repressed by IE2 during CMV infection.
  • Vector type
    Mammalian Expression, Lentiviral, Synthetic Biology ; HIV-1 based replication defective lentivirus vector
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
    human cytomegalovirus UL148 fused to HA epitope
  • Alt name
  • Species
    Human betaherpesvirus 5 (human cytomegalovirus)
  • Insert Size (bp)
  • Mutation
    insert matches UL148 from strain TB40/E
  • GenBank ID
  • Promoter Tet responsive element 3G
  • Tag / Fusion Protein
    • HA tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AgeI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer TCAGGTAGTGAACCGTCAG
  • 3′ sequencing primer TGATACTGGGGTTCTAAGGC
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pOUPc backbone was generated by making extensive modifications to pInducer20 (Addgene Plasmid #44012)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOUPc-UL148-HA was a gift from Jeremy Kamil (Addgene plasmid # 179343 ; ; RRID:Addgene_179343)
  • For your References section:

    The Human Cytomegalovirus Endoplasmic Reticulum-Resident Glycoprotein UL148 Activates the Unfolded Protein Response. Siddiquey MNA, Zhang H, Nguyen CC, Domma AJ, Kamil JP. J Virol. 2018 Sep 26;92(20). pii: JVI.00896-18. doi: 10.1128/JVI.00896-18. Print 2018 Oct 15. 10.1128/JVI.00896-18 PubMed 30045994
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