We narrowed to 6,087 results for: nls

1. pIVT-Bxb1

   Plasmid

   #232746

   Purpose

   IVT template for making modRNA encoding Bxb1

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-iCre

   Plasmid

   #89573

   Purpose

   Expresses iCre under pCAG

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   iCre
   

   Use

   Cre/Lox and Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKI1.1R

   Plasmid

   #85808

   Purpose

   CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

   Depositor

   Tetsuya Higashiyama

   Article

   Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

   Insert

   human-codon-optimized SpCas9
   

   Use

   CRISPR

   Tags

   FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   AtRPS5A

   Available since

   Jan. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FokI-dCas9

   Plasmid

   #52970

   Purpose

   Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

   Depositor

   David Liu

   Article

   Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Fok1 fused to dCas9
   

   Use

   CRISPR

   Tags

   Fok1 nuclease (homodimeric wildtype) and NLS

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   CMV

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-Cre-IRES2-GFP

   Plasmid

   #26646

   Purpose

   Mammalian coexpression of nls-Cre and EGFP from the CAG promoter

   Depositor

   Anjen Chenn

   Article

   Woodhead et al J Neurosci. 2006 Nov 29;26(48):12620-30.

   Insert

   Cre
   

   Use

   Cre/Lox

   Tags

   GFP and IRES2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-BE3

   Plasmid

   #73021

   Purpose

   Expresses BE3 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLSC-5

   Plasmid

   #62889

   Purpose

   Lenti vector for expression of inducible split Cas9

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

   Inserts

   SpCas9(574-1368)
   SpCas9(2-573)
   

   Use

   CRISPR and Lentiviral

   Tags

   FFBP12, FRB, NES PTK2, NLS SV40, and P2A

   Expression

   Mammalian

   Mutation

   *see comment below

   Promoter

   EFS

   Available since

   March 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-Brainbow-2.1 R

   Plasmid

   #18723

   Depositor

   Joshua Sanes

   Article

   Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

   Insert

   hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-BE2

   Plasmid

   #73020

   Purpose

   Expresses BE2 in mammalian cells

   Depositor

   David Liu

   Article

   Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

   Insert

   BE2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMJ920

    Plasmid

    #42234

    Depositor

    Jennifer Doudna

    Article

    Jinek et al elife. 2013;2:e00471. doi: 10.7554/eLife

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    GFP, HA epitope, and NLS (nuclear localization si…

    Expression

    Mammalian

    Mutation

    codon-optimized synthetic DNA sequence

    Promoter

    CMV

    Available since

    March 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. LV-Cre pLKO.1

    Plasmid

    #25997

    Purpose

    Lentiviral expression of nls-Cre from the CMV promoter

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cherry-LacRep

    Plasmid

    #18985

    Depositor

    Mirek Dundr

    Article

    Dundr et al J Cell Biol. 2007 Dec 17. 179(6):1095-10

    Insert

    LacI
    

    Use

    Tags

    AviTag, SV40 NLS, and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTPGI_dCas9_VP64

    Plasmid

    #49013

    Purpose

    encodes yeast-optimized dCas9_VP64 synthetic transcription factor

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    dCas9_VP64 (codon-optimized for expression in S. cerevisiae)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Promoter

    pTPGI (galactose+aTc inducible)

    Available since

    Jan. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pICH47742:FCP:Cas9YFP

    Plasmid

    #85986

    Purpose

    Golden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoter

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR; Golden gate assembly

    Tags

    SV40 NLS, YFP

    Expression

    Mutation

    Promoter

    FCP

    Available since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-VCre

    Plasmid

    #89575

    Purpose

    Expresses VCre under pCAG

    Depositor

    Wilson Wong

    Article

    Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

    Insert

    VCre recombinase
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pdCas9-NED

    Plasmid

    #109358

    Purpose

    Mammalian vector for expressing effector domains fused to dCas9.

    Depositor

    Antal Kiss

    Article

    Goubert et al Methods Mol Biol. 2018;1767:395-415. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    dCas9, SV40 NLS, 3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. M-ST1cas

    Plasmid

    #48669

    Purpose

    Mammalian S. thermophilus #1 Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL1394

    Plasmid

    #129522

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyuis4, PyBiP-minimal promoter, 3rd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFLAG-NLS

    Expression

    Mutation

    Promoter

    Available since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. Thy1-Brainbow-2.1 R

    Plasmid

    #18727

    Depositor

    Joshua Sanes

    Article

    Livet et al Nature. 2007 Nov 1. 450(7166):56-62.

    Insert

    hrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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20. M-NMcas

    Plasmid

    #48670

    Purpose

    Mammalian N. meningitidis Cas9 expression, human optimized

    Depositor

    George Church

    Article

    Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only