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Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors.
Baeumler TA, Ahmed AA, Fulga TA
Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101101pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPREEncodes dCas9(N) fused to transmembrane tether
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101102pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether
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101103pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1
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101104pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPREEncodes dCas9(N) fused to VEGFR2
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101105pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)Encodes dCas9(C) (without VP64) fused to VEGFR1
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101106pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPREEncodes FKBP-dCas9(N) fused to VEGFR2
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101107pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2
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101108pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPREEncodes dCas9(N) fused to BDKRB2
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101109pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFREncodes dCas9(N)-DHFR fused to BDKRB2
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101110pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2
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101111pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1Encodes dCas9(C)-VP64 with SAM components fused to AVPR2
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101112pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPREEncodes dCas9(N) fused to AVPR2
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101113pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1Encodes dCas9(C)-VP64 with SAM components fused to CXCR4
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101114pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPREEncodes dCas9(N) fused to CXCR4
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101115pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1Encodes dCas9(C)-VP64 with SAM components fused to LPAR1
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101116pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPREEncodes dCas9(N) fused to LPAR1
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