Addgene: Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors.

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Tudor Fulga
Baeumler et al

Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors.
Baeumler TA, Ahmed AA, Fulga TA
Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
101101	pTMt_NES_TCS(Q’G)_HA-dCas9(N)_P2A-Puro-WPRE	Encodes dCas9(N) fused to transmembrane tether
101102	pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1	Encodes dCas9(C)-VP64 with SAM components fused to transmembrane tether
101103	pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1	Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1
101104	pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE	Encodes dCas9(N) fused to VEGFR2
101105	pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)	Encodes dCas9(C) (without VP64) fused to VEGFR1
101106	pVEGFR2_TEV(N)_NES_TCS(Q'L)_FKBP_HA-dCas9(N)_P2A_Puro-WPRE	Encodes FKBP-dCas9(N) fused to VEGFR2
101107	pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1	Encodes dCas9(C)-VP64 with SAM components fused to BDKRB2
101108	pBDKRB2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE	Encodes dCas9(N) fused to BDKRB2
101109	pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR	Encodes dCas9(N)-DHFR fused to BDKRB2
101110	pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)_T2A_DHFR-PCP-VP64	Encodes dCas9(C) with DHFR-PP7-VP64 fused to BDKRB2
101111	pAVPR2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1	Encodes dCas9(C)-VP64 with SAM components fused to AVPR2
101112	pAVPR2_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE	Encodes dCas9(N) fused to AVPR2
101113	pCXCR4_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1	Encodes dCas9(C)-VP64 with SAM components fused to CXCR4
101114	pCXCR4_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE	Encodes dCas9(N) fused to CXCR4
101115	pLPAR1_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1	Encodes dCas9(C)-VP64 with SAM components fused to LPAR1
101116	pLPAR1_TCS(Q’L)_HA-dCas9(N)_P2A-Puro-WPRE	Encodes dCas9(N) fused to LPAR1

Antibodies from Article