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pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
(Plasmid #101107)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101107 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTMt_TCS(Q’G)_NLSHA- dCas9(C)-VP64_T2A_MCP-P65-HSF1
  • Modifications to backbone
    A sequence containing the membrane localisation signal, FLAG tag, BDKBR2 coding sequence and the V2 tail were PCR amplified from plasmid BDKBR2-Tango (gift from Bryan Roth, Addgene plasmid #66230) and used to replace the TMt in pTMt_TCS(Q’G)_NLSHA- dCas9(C)-VP64_T2A_MCP-P65-HSF1.
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BDKRB2-dCas9(N)
  • Tags / Fusion Proteins
    • Flag (N terminal on insert)
    • HA

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    BDKBR2-Tango (gift from Bryan Roth, Addgene plasmid #66230); pTMt_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1 was a gift from Tudor Fulga (Addgene plasmid # 101107 ; http://n2t.net/addgene:101107 ; RRID:Addgene_101107)
  • For your References section:

    Engineering Synthetic Signaling Pathways with Programmable dCas9-Based Chimeric Receptors. Baeumler TA, Ahmed AA, Fulga TA. Cell Rep. 2017 Sep 12;20(11):2639-2653. doi: 10.1016/j.celrep.2017.08.044. 10.1016/j.celrep.2017.08.044 PubMed 28903044