We narrowed to 9,801 results for: pET
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Plasmid#133973PurposeHeterologous, copper-inducible expression of (-)-α- bisabolol synthase from Matricaria recutita (MrBBS) in Synechocystis sp. PCC 6803. The MrBBS CDS is fused to a C-termial StrepII-tag.DepositorInsertMrBBS:StrepII
UseSynthetic BiologyTagsStrepIIExpressionBacterialMutationinserted gene is codon optimized for Synechocyst…PromoterPpetE:BCD2Available SinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET29NK-LGK/mGFPmut3
Plasmid#81168PurposeGFP fusion to levoglucosan kinaseDepositorInsertLevoglucosan kinase wild-type
TagsHis6x and mGFPmut3ExpressionBacterialPromoterT7Available SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a HAT epitope
Plasmid#129445Purposeexpression of HAT epitopeDepositorInsertHAT epitope
ExpressionBacterialAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET51b-NAD-Luc
Plasmid#135627PurposeBacterial expression of a biosensor for measuring NAD+ levels at the point of care (V0)DepositorInsertNAD-Luc sensor
TagsHisx10 and StrepExpressionBacterialPromoterT7Available SinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15b-mLin28A-cHIS
Plasmid#137998PurposeLin28-His6 was overexpressed in Escherichia coli Rosetta (DE3) cellsDepositorInsertmouse Lin28A, CSD and ZnFs (residues 36–186)
TagsC-terminal His6 tagExpressionBacterialAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a His6Flag2AUF1(p40)
Plasmid#135940PurposeExpresses AUF1p40 in E. coliDepositorInsertheterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
TagsFlag2 and His6ExpressionBacterialPromoterT7Available SinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-C-terminal YPet
Plasmid#128027PurposeMammalian expression vector with C terminal YPet tagDepositorTypeEmpty backboneTagsYPetExpressionMammalianAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB1C3_YPet-PCP (y-plasmid)
Plasmid#127637PurposeEncodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.DepositorInsertYPet
UseSynthetic BiologyTagsRNA binding peptide: PP7 coat protein (PCP)ExpressionBacterialPromoterpJ21306Available SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-HCA_sensor
Plasmid#127181PurposeBacterial expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
TagsHisTag and StrepTagExpressionBacterialPromoterT7Available SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-G51PEKN
Plasmid#118582PurposeBacterial expression of E.coli DHFR G51PEKN mutantDepositorInsertDHFR
ExpressionBacterialMutationG51 mutated to P51; Glu-Lys-Asn residues inserted…PromoterT7Available SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a_6HT-TBP6.7(R49A)
Plasmid#112726PurposeArg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.DepositorInsert6His-TEV-TBP6.7(R49A)
Tags6His-TEVExpressionBacterialMutationR49APromoterT7Available SinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
ExpressionBacterialMutationY100 mutated to F100PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hTIG3-1-164
Plasmid#100724PurposeFull-length human TIG3 in E.coli expression vectorDepositorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28b-hTIG3-1-132
Plasmid#100720PurposeExpression of N-terminal region of human TIG3 in E.coliDepositorAvailable SinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET26-CNTN149S/F366A
Plasmid#100165Purposerecombinant expression of target protein as an MBP-His tag fusionat the N-terminus of target proteinDepositorInsertConcentrative nucleoside transporter
TagsMBP-HisExpressionBacterialMutationN149S, F366APromoterT7Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-his-3C_Clu3B6p
Plasmid#100093Purposeexpresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptideDepositorInsert14-3-3 sigma human (SFN Human)
TagsHSPB6 peptide and His6ExpressionBacterialMutation75-77 residues changed to AAA to facilitate cryst…PromoterT7Available SinceSept. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(81-437)
Plasmid#87734PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-80PromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
D5 gene in PETCON vector
Plasmid#87626Purposeexpresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast displayDepositorInsertDesigned allosteric Zn2+ and fluorescein binding antibody scFv D5
Tags3x(G4S) Linker and cMyc tag on backboneExpressionYeastAvailable SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-H74A-PvuRts1I
Plasmid#86894Purposeexpress H74A variant PvuRts1I in ER2566 strainDepositorAvailable SinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET29b-EPN01(∆L1+∆L2)-His
Plasmid#84511PurposeE.coli version of EPN01(∆L1+∆L2)DepositorInsertEPN01(∆L1+∆L2)-His
TagsHis tagExpressionBacterialAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only