We narrowed to 4,063 results for: biorxiv

1. pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

   Plasmid

   #240228

   Purpose

   Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

   Plasmid

   #240229

   Purpose

   Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Insect

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUAS-spatzle-HA

   Plasmid

   #240235

   Purpose

   Expression of spatzle-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.

   Depositor

   Justin Bosch, Norbert Perrimon

   Article

   Bosch et al bioRxiv 2025.07.09.659702; doi: https://

   Insert

   spatzle (spz Fly)
   

   Tags

   HA-spGFP11

   Expression

   Insect

   Promoter

   UAS

   Available Since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCambia1300_cLUC-NLRb

   Plasmid

   #233527

   Purpose

   Expresses split luciferase fusion cLUC-NLRb in planta

   Depositor

   Brande Wulff

   Article

   Chen et al bioRxiv 2024.08.30.610287

   Insert

   Sr62NLRb
   

   Tags

   C-terminal fragment of Luciferase

   Expression

   Plant

   Promoter

   35S

   Available Since

   June 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. MKC84_HBB(HBA1reg-HBA1)

   Plasmid

   #232410

   Purpose

   AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA1 cassette.

   Depositor

   Kyle Cromer

   Article

   Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

   Insert

   Hemoglobin Subunit Alpha 1 (HBA1 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. MKC131_CCR5(SFFV-synEPOR-2A-YFP)

   Plasmid

   #232412

   Purpose

   AAV production plasmid for SFFV(synEPOR) vector from Figs. 2-3 that mediates HDR at CCR5 locus using CCR5 gRNA. YFP is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

   Depositor

   Kyle Cromer

   Article

   Shah et al bioRxiv 2024.04.12.589260

   Insert

   Truncated Erythropoietin Receptor (EPOR Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. MKC175_HBA1reg(synEPOR)

   Plasmid

   #232413

   Purpose

   AAV production plasmid for HBA1 UTRs vector from Fig. 3 that mediates HDR at HBA1 locus using HBA1 sg5 gRNA. HBB gene includes introns; HBA1 UTRs flank H+C15BA1 cassette. HAs are ~400bp each.

   Depositor

   Kyle Cromer

   Article

   Shah et al bioRxiv 2024.04.12.589260

   Insert

   Truncated Erythropoietin Receptor (EPOR Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. MKC83_HBB(HBBreg-HBA1)

   Plasmid

   #232409

   Purpose

   AAV production plasmid for HBB UTRs vector from Fig. 3 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBB UTRs flank HBA1 cassette.

   Depositor

   Kyle Cromer

   Article

   Chu et al bioRxiv [Preprint]. 2024 May 7:2023.09.0

   Insert

   Hemoglobin Subunit Alpha 1 (HBA1 Human)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   April 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. A1-LCD allW D262V

   Plasmid

   #234616

   Purpose

   Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262V with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

   Depositor

   Tanja Mittag

   Article

   Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

   Insert

   hnRNPA1_LCD_allW D262V (HNRNPA1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   C-terminal domain (186-320) bearing familial ALS …

   Promoter

   T7

   Available Since

   April 15, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. A1-LCD allW

    Plasmid

    #234609

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_allW (HNRNPA1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    C-terminal domain (186-320) with all aromatic ami…

    Promoter

    T7

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. A1-LCD allW D262N

    Plasmid

    #234615

    Purpose

    Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) D262N with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

    Depositor

    Tanja Mittag

    Article

    Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

    Insert

    hnRNPA1_LCD_allW D262N (HNRNPA1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    C-terminal domain (186-320) bearing familial ALS …

    Promoter

    T7

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p5E-Dll4-F2-E1B-B-Globin (JDW 1366)

    Plasmid

    #229843

    Purpose

    A gateway compatible 5' entry clone containing the murine Dll4 F2 arterial specific enhancer upstream of a minimal E1b promoter and intron for arterial and endocardial expression.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Dll4-F2 (exon3)
    

    Use

    Gateway entry clone

    Available Since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-U6/TO-shEsr1-CMV-TetR-eGFP

    Plasmid

    #233298

    Purpose

    DOX-inducible U6 driven shRNA against mouse Esr1

    Depositor

    Dayu Lin

    Article

    Golden et al bioRxiv [Preprint]. 2024 Sep 25:2023.12.

    Insert

    Esr1 shRNA (Esr1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. MKC177_CCR5(PGK-synEPOR)

    Plasmid

    #232415

    Purpose

    AAV production plasmid for PGK(synEPOR) vector from Figs. 3-5 that mediates HDR at CCR5 locus using CCR5 gRNA. synEPOR is followed by BGH polyA. AAV genome is flanked by AAV2 ITRs.

    Depositor

    Kyle Cromer

    Article

    Shah et al bioRxiv 2024.04.12.589260

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    March 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MKC176_EPOR(2A-synEPOR)

    Plasmid

    #232414

    Purpose

    AAV production plasmid for EPOR(synEPOR) vector from Figs. 3-5 that mediates HDR at EPOR locus using EPOR gRNA. synEPOR is codon diverged to prevent premature recombination with EPOR locus.

    Depositor

    Kyle Cromer

    Article

    Shah et al bioRxiv 2024.04.12.589260

    Insert

    Truncated Erythropoietin Receptor (EPOR Human)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTol2-Ubi-lexA-oMDC-pA-lexO-cFos-nlsGFP-pA (JDW 1382)

    Plasmid

    #229813

    Purpose

    A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator and a c-fos minimal promoter.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    LexA-mODC-LexAOp-cFos
    

    Use

    Tol2 based expression vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

    Plasmid

    #229817

    Purpose

    A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-Tet-Off-FLEX-DEST (JDW 1186)

    Plasmid

    #229820

    Purpose

    An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Type

    Empty backbone

    Use

    AAV

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

    Plasmid

    #229824

    Purpose

    A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Luciferase-P2A-H2A-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p5E-Crestin-pro (JDW 1320)

    Plasmid

    #229830

    Purpose

    A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Crestin promoter, c-fos minimal promoter, and b-globin intron
    

    Use

    Gateway entry clone

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge