We narrowed to 4,014 results for: biorxiv
-
Plasmid#216734PurposeAAV plasmid containing sgCTG (target sequence: (CUG)6) driven by a U6 promoter and eGFP under the control of the CMV promoter. Used for in vivo experiments.DepositorArticleInsertsgCTG
UseAAV and CRISPRExpressionMammalianPromoterU6 and CMVAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-nEF-SpCas9D10A nickase
Plasmid#216736PurposeExpresses SpCas9-D10A nickase from a nEF promoter. For AAV packaging. Derived from pAAV-nEF-SpCas9 (Addgene #87115)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRExpressionMammalianMutationD10APromoterEF-1-alpha coreAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-CSK-P2A-mCherry BlastR
Plasmid#186742PurposeDox inducible CSK-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti_EF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP_HygroR
Plasmid#186744PurposeEF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP expression construct cloned into a lentiviral backboneDepositorInsertEF1a-scFv-SBNO1-KBP250-NFEL1-GSTGGT-KRT40-T2A-MCP-P65-HSF1-P2A-BFP
UseLentiviralTagsP2A-BFPExpressionMammalianAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
SYKA-c022
Plasmid#175490PurposeProtein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.DepositorAvailable SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-DIO-PinkyCaMP
Plasmid#232857PurposeAn AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)DepositorInsertPinkyCaMP
UseAAVExpressionMammalianPromoterhSynAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Anti-amyloid-β 40/42 nanobody (R3VQ) with sortase tag
Plasmid#233217PurposeMammalian epression of anti-amyloid-β 40/42 nanobody (R3VQ) with a sortase tag for direct dye conjugation.DepositorInsertAnti-amyloid-β 40/42 nanobody (R3VQ)
TagsSortase tag, His tagExpressionMammalianAvailable SinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato
Plasmid#234952PurposeHuman codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K36M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225703PurposeLentiviral expression of AID degron tagged H3.3(K36M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK36MPromoterpEF1AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo
Plasmid#234953PurposeHuman codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIII pm
Plasmid#218100PurposePhagemid that expresses LacI, and pIII under the Lac promoterDepositorInsertpIII
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iGluSnFR4f
Plasmid#234444PurposeBacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)DepositorInsertiGluSnFR4f
Tags6xHis-Xpress epiExpressionBacterialPromoterT7Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K36M)-3AID-HA-2A-mCherryBSD
Plasmid#225699PurposeLentiviral expression of AID degron tagged H3.3(K36M) and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationK36MPromoterpEF1AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo
Plasmid#234954PurposeHuman codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmidDepositorInserthuman codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase
UseLentiviralTagsFLAGExpressionMammalianPromoterEF1aAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC62_HBB(HBA1reg-HBA1-2A-YFP)
Plasmid#232408PurposeAAV production plasmid for HBA1-2A-YFP vector from Fig. 2 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank full HBA1-2A-YFP cassette.DepositorAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEtfdh CRISPR_2
Plasmid#232313PurposeMouse Etfdh Gene Knockout (gRNA 2)DepositorInsertEtfdh-targeting gRNA (Etfdh Mouse)
UseLentiviralAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
mEtfdh CRISPR_1
Plasmid#232312PurposeMouse Etfdh Gene Knockout (gRNA 1)DepositorInsertEtfdh-targeting gRNA (Etfdh Mouse)
UseLentiviralAvailable SinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pIV pm
Plasmid#218101PurposePhagemid that expresses LacI, and pIV under the Lac promoterDepositorInsertpIV
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K27M)-3AID-HA-2A-mTurquoiseBSD
Plasmid#225702PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mTurquoise fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mTurquoiseExpressionMammalianMutationK27MPromoterpEF1AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iGluSnFR4s
Plasmid#234443PurposeBacterial expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow)DepositorInsertiGluSnFR4s
Tags6xHis-Xpress epiExpressionBacterialPromoterT7Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only