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We narrowed to 5,230 results for: golden gate

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Showing: 3821 - 3840 of 5230 results

  1. pEvolvR-mCherry-HF1-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249075
    Purpose
    Expresses nucleus-localized HF1-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    HF1-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249075-plasmid-map-sequence-id-496315

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  2. pEvolvR-mCherry-Hypa-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249076
    Purpose
    Expresses nucleus-localized Hypa-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    Hypa-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249076-plasmid-map-sequence-id-496314

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  3. pEvolvR-mCherry-SpRY-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249074
    Purpose
    Expresses nucleus-localized SpRY-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    SpRY-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249074-plasmid-map-sequence-id-496313

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  4. pEvolvR-mCherry-enCas9(D10A)-PolI5M

    Plasmid
    #249060
    Purpose
    Expresses nucleus-localized enCas9 (D10A) fused to PolI5M and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Depositor
    John Dueber, David Schaffer
    Publication
    Hurtado et al Nat Commun. 2025 Apr 19;16(1):3723. doi:
    Insert
    enCas9-PolI5M
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Dec. 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    249060-plasmid-map-sequence-id-495988

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  8. pNDMS291

    Plasmid
    #248098
    Purpose
    sfCFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfCFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248098-plasmid-map-sequence-id-495377

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  9. pNDMS290

    Plasmid
    #248097
    Purpose
    sfYFP fluorescent reporter for synthetic Rhizobium nifH promoter
    Depositor
    Barney Geddes
    Insert
    sfYFP
    Expression
    Bacterial
    Promoter
    Rlv_PsnifH
    Available since
    Dec. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    248097-plasmid-map-sequence-id-495376

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  15. AcrVA1_cpGR2(GGS5)-S103ΔELS

    Plasmid
    #246050
    Purpose
    Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS; enables chemogenetic control of MbCas12a
    Depositor
    Dominik Niopek
    Publication
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    AcrVA1_cpGR2-S103ΔELS
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    ΔELS
    Available since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246050-plasmid-map-sequence-id-495344

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  16. AcrVA1_cpGR2(GGS5)-S103ΔELS_(GGS)2-(GGS)2

    Plasmid
    #246051
    Purpose
    Expression of AcrVA1-cpGR2 hybrid in mammalian cells; insertion before S103 with deletion of surrounding amino acids ELS and GGS2 linkers; enables chemogenetic control of MbCas12a
    Depositor
    Dominik Niopek
    Publication
    Brenker et al Nucleic Acids Res. 2025 Jul 19;53(14):gk
    Insert
    AcrVA1_cpGR2-S103ΔELS
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Mutation
    ΔELS
    Available since
    Dec. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    246051-plasmid-map-sequence-id-495343

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Showing: 3821 - 3840 of 5230 results