We narrowed to 4,014 results for: biorxiv
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Plasmid#159751PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEM-2t
Plasmid#159752PurposeCRISPR/Cas9 genome editing in plants; a template for PCR-derived fragments used in the assembly of polycistronic transcripts, where sgRNAs are separated by an Arabidopsis alanine tRNA.DepositorArticleInsertsgRNA
UseCRISPRAvailable SinceOct. 6, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET20b-mPTER
Plasmid#222189PurposeBacterial expression of mouse PTER proteinDepositorAvailable SinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
LacI-pLacI-pII pm
Plasmid#218099PurposePhagemid that expresses LacI, and pII under the Lac promoterDepositorInsertpII
ExpressionBacterialPromoterLac promoterAvailable SinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
LMPd Ametrine Got2 shRNA#3
Plasmid#220593PurposeRetroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffoldDepositorInsertGot2 shRNA (Got2 Mouse)
UseRetroviralAvailable SinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast
Plasmid#216733PurposeExpresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.DepositorArticleInsertCas9-D10A nickase and sgCTG
UseCRISPR and LentiviralExpressionMammalianMutationD10APromoterU6 and CAGAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR
Plasmid#186740PurposeDox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance geneDepositorUseLentiviralTagsRAB10-T2A-RABIF-P2A-mCherryExpressionMammalianAvailable SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
10_T7-SP-CD8tm-mScarlet3
Plasmid#225937PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domainDepositorInsertSP-CD8tm-mScarlet3
UseIn vitro transcriptionPromoterT7Available SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD
Plasmid#225698PurposeLentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinRDepositorUseLentiviral and Synthetic BiologyTags3xAID HA and T2A-mCherryExpressionMammalianMutationK27MPromoterpEF1AAvailable SinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX551-miniCMV-SpCas9D10A nickase
Plasmid#216735PurposeExpresses SpCas9-D10A nickase from a miniCMV promoter. For AAV packaging. Derived from pX551-miniCMV-SpCas9, which was a gift from Alex Hewitt (Addgene #107031)DepositorArticleInsertCas9-D10A nickase
UseAAV and CRISPRExpressionMammalianMutationD10APromoterT7 and mini-CMVAvailable SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTUU
Plasmid#159749PurposeUbiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by mTomato fluoresce.DepositorArticleInsertgRNA scaffold
UseCRISPRExpressionPlantAvailable SinceOct. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSico-syn-mGreenLantern:KASH-TVA-oG-6xMBS-WPRE-bGHpA
Plasmid#231010PurposeLentiviral helper virus for G-deleted rabies tracing. mGreenLantern:KASH fluorescence is not visible without immunostaining.DepositorInsertsUseLentiviralTagsKASHMutationPlease note that mGreenLantern-KASH is not visibl…Promoterhuman synapsin IAvailable SinceDec. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAPLe4 PCMTD1 F57 F217 F220
Plasmid#246505PurposeRecombinant protein expression of PCMTD1 F57 F217 F220DepositorInsertProtein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
TagsHis-tagExpressionBacterialMutationResidue 312 is Isoleucine in this plasmid but occ…Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMAPLe4 PCMTD1 T62 H67 M68
Plasmid#246506PurposeRecombinant protein expression of PCMTD1 T62 H67 M68DepositorInsertProtein-L-isoaspartate O-methyltransferase domain-containing protein 1 (PCMTD1 Human)
TagsHis-tagExpressionBacterialMutationResidue 312 is Isoleucine in this plasmid but occ…Available SinceOct. 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-eGFP-VSV
Plasmid#242781PurposeAAV transfer plasmid expressing eGFP-VSV under a CAG promoter.DepositorInsertEGFP
UseAAVTagsVSV G tagPromoterCAGAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_ISL1-LHX3_Down-Tandem
Plasmid#241395PurposeBxb1-GA donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression of ISL1 and LHX3DepositorTagsNLSExpressionMammalianPromoterTRE; CAGAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hhN-HA
Plasmid#240236PurposeExpression of hhN-HA under control of UAS promoter. Can be used to generate transgenic fly lines by white+ fly eye selection and phiC31 integration.DepositorInserthedgehog N-terminal fragment (hh Fly)
TagsHA-spGFP11ExpressionInsectMutationaa 1-257PromoterUASAvailable SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
pENTR_hhN-HA
Plasmid#240225PurposeGateway entry clone with N-terminal hedgehog (hhN) tagged with HA (contains stop codon)DepositorInserthedgehog N-terminal fragment (hh Fly)
UseGateway shuttle vectorTagsHA-spGFP11Mutationaa 1-257Available SinceJuly 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
TRAV38-2/DV8
Plasmid#237992PurposeEncodes TRAV38-2/DV8 allele, P2A, and TRBC to generate TCRs via cloningDepositorAvailable SinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
MKC106_HBB(HBA1reg-HBA1-IRES-tEPOR)
Plasmid#232411PurposeAAV production plasmid for HBA+tEPOR vector from Figs. 4-6 that mediates HDR at HBB locus using HBB sg7 gRNA. HBA gene includes HBA1 exons and introns; HBA1 UTRs flank HBA+tEPOR cassette.DepositorAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only