We narrowed to 4,704 results for: golden gate
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Plasmid#246453PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV823
Plasmid#246452PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV746
Plasmid#246449PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceNov. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV745
Plasmid#246448PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEV785
Plasmid#246451PurposeThe plasmid comprises an indicible transcriptional unit encoding Gal4-VPRDepositorInsertGal4-VPR
UseSynthetic BiologyMutationMutated Kozak sequencePromoterminiYBAvailable SinceOct. 31, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1729
Plasmid#246063PurposeBacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a oriDepositorInsertPanoptes locus
ExpressionBacterialPromoterpTet promoterAvailable SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTB105
Plasmid#236778PurposeBackbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.DepositorTypeEmpty backboneUseCRISPRAvailable SinceOct. 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
CAG-mRuby2(miRE.FF4)-TS.FF6x1-bGH
Plasmid#235297PurposeComMAND open-loop circuit regulating mRuby2, CAG-drivenDepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterCAG (synthetic cytomegalovirus early enhancer + c…Available SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF4)-TS.FF4x4-bGH
Plasmid#235295PurposeComMAND closed-loop circuit regulating mRuby2 with 4 copies of microRNA target sitesDepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
SMT503 - S77A
Plasmid#226842PurposePlasmid that encodes a B. Subtilis lipase (CLIP-BsLipA-eGFP) gene fusion with the S77A catalytic mutant. Compatible with PURExpress for in vitro transcription/translation. ChlorampDepositorInsertLipase A
UseSynthetic BiologyTagsSNAP and eGFPExpressionBacterialMutationR33Q, D34N, K35D, S77A, K112D, M134D, Y139C, I157MPromoterT7Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
SMT503
Plasmid#226840PurposePlasmid that encodes a B. Subtilis lipase (BsLipA) gene fusion with N-terminal SNAP and C-Terminal eGFP under a T7 promoter. Compatible with PURExpress for in vitro transcription/translation. ChlorampDepositorInsertLipase A
UseSynthetic BiologyTagsSNAP and eGFPExpressionBacterialMutationR33Q, D34N, K35D, K112D, M134D, Y139C, I157MPromoterT7Available SinceAug. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3777_pGEEC584_pShip-EFS(promoter)-CMV(UTR)-mRuby2-bGH
Plasmid#239746PurposePlasmid expressing mRuby2 from the EFS canonical promoter with the CMV 5' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMKR07
Plasmid#240097PurposeA CRISPR‑Cas9 system for knock‑out and knock‑in of high molecular weight DNA enables module‑swapping of the pikromycin synthase in its native hostDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialPromoterermE* promoterAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3666_pGEEC576_pShip-EFS-ZF43a(inducible)-P2A-iRFP720-bGH
Plasmid#239740PurposePlasmid expressing a rapamycin-inducible transcriptional activatorDepositorInsertNLS-FXBP-ZF43-P2A-NES-VP64-FRB-P2A-iRFP720
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3831_pGEEC579_pShip-UbC(no_intron)-mRuby2-bGH
Plasmid#239742PurposePlasmid expressing mRuby from a UbC promoter lacking an intron, used for modRNA productionDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3774_pGEEC580_pShip-EF1a(no_intron)-mRuby2-bGH
Plasmid#239743PurposePlasmid expressing mRuby from an EF1a promoter lacking an intron, used for modRNA productionDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3775_pGEEC582_pShip-hPGK(promoter)-CMV(UTR)-mRuby2-bGH
Plasmid#239744PurposePlasmid expressing mRuby2 from the hPGK canonical promoter with the CMV 5' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3776_pGEEC583_pShip-hPGK(promoter)-EFS(UTR)-mRuby2-bGH
Plasmid#239745PurposePlasmid expressing mRuby2 from the hPGK canonical promoter with the EFS 5' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3778_pGEEC585_pShip-EFS(promoter)-hPGK(UTR)-mRuby2-bGH
Plasmid#239747PurposePlasmid expressing mRuby2 from the EFS canonical promoter with the hPGK 5' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3779_pGEEC586_pShip-CMV(promoter)-EFS(UTR)-mRuby2-bGH
Plasmid#239748PurposePlasmid expressing mRuby2 from the CMV canonical promoter with the EFS 5' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only