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pFRT_TO_FLAGHA_PRKRA_sgRNA_PAM_mutant

Plasmid

#106112

Purpose

Inducible gene expression of FlagHA-PRKRA sgRNA PAM mutant

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Tags

Flag and HA

Expression

Mammalian

Mutation

silent mutation of Gly32 (GGG-->GGT)

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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PACT deltaD1

Plasmid

#251698

Purpose

PRKRA (deletion of 1-124) protein expression construct in E. coli

Depositor

Sun Hur

Publication

Ahmad et al Nat Commun. 2025 Apr 8;16(1):3325. doi:

Insert

PRKRA (PRKRA Human)

Expression

Bacterial

Mutation

deletion of 1-124

Available since

April 28, 2026

Availability

Academic Institutions and Nonprofits only

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px458_2A_GFP_sgRNA_PRKRA

Plasmid

#106109

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting PRKRA

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA targeting PRKRA (PRKRA Human)

Use

CRISPR

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pFL-SV40-PACT 5'-UTR

Plasmid

#115351

Purpose

firefly luciferase (Fluc) reporter containing the 5’-UTR of PACT

Depositor

Ming-Chih Lai

Publication

Lai et al FEBS J. 2016 Jan;283(1):88-101. doi: 10.

Insert

PACT 5'-UTR (PRKRA Human)

Use

Luciferase

Expression

Mammalian

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSV40_HA-mPACT

Plasmid

#120537

Purpose

protein expression

Depositor

Petr Svoboda

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PACT (Prkra Mouse)

Expression

Mammalian

Available since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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PRKRA gRNA (BRDN0001146443)

Plasmid

#76299

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKRA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKRA (PRKRA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKRA gRNA (BRDN0001147957)

Plasmid

#76300

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKRA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKRA (PRKRA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PRKRA gRNA (BRDN0001146546)

Plasmid

#76301

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKRA

Depositor

John Doench, David Root

Publication

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKRA (PRKRA Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mcherry(C1)_RepoMan/RAXA

Plasmid

#46275

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Publication

Trinkle-Mulcahy et al J Cell Biol. 2006 Feb 27;172(5):679-92.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RepoMan (CDCA2 Human)

Tags

mcherry

Expression

Mammalian

Mutation

V393A/F395A

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only
pFRT-TODestFLAGHAhFMRPiso1

Plasmid

#48690

Purpose

Expresses hFMRP iso 1

Depositor

Thomas Tuschl

Publication

Ascano M et al Nature. 2012 Dec 20;492(7429):382-6. doi

Insert

FlagHA FMR1 Iso 1 (FMR1 Human)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV, 2x Tet

Available since

May 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pEGFP(C1)-RepoMan/RAXA

Plasmid

#44213

Depositor

Angus Lamond, Laura Trinkle-Mulcahy

Publication

Trinkle-Mulcahy et al J Cell Biol. 2006 Feb 27;172(5):679-92.

Insert

RepoMan (CDCA2 Human)

Tags

GFP

Expression

Mammalian

Mutation

V393A/F395A

Promoter

CMV

Available since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pENTR4_PRKRA

Plasmid

#106110

Purpose

Entry vector for PRKRA

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Gateway Cloning

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RCAS(A) cRaxL (CC#526)

Plasmid

#15193

Depositor

Connie Cepko

Publication

Chen et al Development. 2002 Dec . 129(23):5363-75.

Insert

RaxL

Use

Retroviral; Avian expression

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pBS xenopus paraxial protocadherin

Plasmid

#24687

Depositor

Edward De Robertis

Publication

Kim et al Development. 1998 Dec . 125(23):4681-90.

Insert

xenopus paraxial protocadherin (pcdh8.2.L Frog)

Expression

Bacterial

Available since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only
RCAS(B) En cRaxL HD (CC#520)

Plasmid

#15197

Depositor

Connie Cepko

Publication

Chen et al Development. 2002 Dec . 129(23):5363-75.

Insert

RaxL

Use

Retroviral; Avian expression

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1-Xlrbpa-dsRBD(+6)

Plasmid

#171548

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Publication

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Mutation

a six amino acid_TRLTEG_insertion in the L1 region

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-Xlrbpa

Plasmid

#171547

Purpose

Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

Depositor

Yan Zeng

Publication

Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

Insert

Xlrbpa (prkra.L Frog)

Expression

Bacterial

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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RCAS(B) En cRaxL deltaC (CC#523)

Plasmid

#15196

Depositor

Connie Cepko

Publication

Chen et al Development. 2002 Dec . 129(23):5363-75.

Insert

RaxL

Use

Retroviral; Avian expression

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pcold-6xHis-HRV3Csite-HsPACT

Plasmid

#41097

Depositor

Phillip Zamore

Publication

Fukunaga et al Cell. 2012 Oct 9. pii: S0092-8674(12)011

Insert

PACT (PRKRA Human)

Tags

6xHis-HRV3Csite

Expression

Bacterial

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pWZL Neo Myr Flag PRKRA

Plasmid

#20608

Depositor

William Hahn, Jean Zhao

Publication

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PRKRA (PRKRA Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only