We narrowed to 908 results for: pgem

1. pGEM-myr-tdTomato-SV40pA-FKF

   Plasmid

   #119867

   Purpose

   Donor cassette containing myr-tagged tdTomato and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   myr-tagged tdTomato and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   2x myristoylation

   Expression

   Bacterial

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMT-Tyrosinase-bait-T2a-Cherry-pA

   Plasmid

   #111155

   Purpose

   knock in bait for Axolotl Tyrosinase locus

   Depositor

   Elly Tanaka

   Article

   Fei et al Stem Cell Reports. 2014 Sep 9;3(3):444-5

   Insert

   Tyrosinase-bait-T2a-Cherry-pA
   

   Expression

   Mammalian

   Mutation

   wt

   Available Since

   Aug. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMT-PT2A-Tdtomato-GFP-iRFP670

   Plasmid

   #111814

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Tdtomato
   GFP
   iRFP670
   

   Use

   Cloning intermediate

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. XE169 mouse Wnt-1-myc-pGEM4

   Plasmid

   #16855

   Depositor

   Randall Moon

   Article

   McMahon et al Cell. 1989 Sep 22;58(6):1075-84. doi: 10

   Insert

   Wnt-1-myc (Wnt1 Mouse)
   

   Use

   Xenopus expression

   Tags

   Myc

   Available Since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pGEMHE-MmCENPT-RnHFD-4NS-GFP

   Plasmid

   #237432

   Purpose

   Expresses Mus musculus CENP-T with rat HFD and 4 mouse-specific residues in positions that show signs of adaptive evolution; tagged with GFP at C-term; made for in vitro transcription (T7 promoter)

   Depositor

   Michael Lampson

   Article

   Dudka et al Curr Biol. 2025 Mar 10;35(5):1012-1022.e

   Insert

   Mus musculus CENP-T with Rattus norvegicus HFD and 4 mouse-specific residues in HFD (Cenpt Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   432 A to T; 463 T to I, 470 E to L, 485 L to Q in…

   Promoter

   T7

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEMHE:mTREK-1 (C159A/C219A/C365A/C399A)

   Plasmid

   #224754

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1 (C159A/C219A/C365A/C399A)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Mutation

   C159A/C219A/C365A/C399A

   Promoter

   T7

   Available Since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEMHE-RnCENP-O-298-5NS-GFP

   Plasmid

   #205742

   Purpose

   Expresses chimeric rat CENP-O with mouse CENP-O C-terminal RWD domain and 5 mouse-specific point mutations; C-term tagged with GFP under T7 promoter - designed for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

   Insert

   CENP-O (Cenpo, Cenpo Rat, Mouse)
   

   Use

   In vitro transcription

   Tags

   5 glycines linker followed by GFP

   Expression

   Mammalian

   Mutation

   Chimeric rat CENP-O with mouse CENP-O C-terminal …

   Promoter

   T7

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM-T Easy-Cas9-nls-CDS1

   Plasmid

   #160231

   Purpose

   Cas9-nuclear localization, level 0 of MoClo Golden Gate position CDS1

   Depositor

   Matias Kirst

   Article

   Triozzi et al Tree Physiol. 2021 May 7. pii: 6270869.

   Insert

   Cas9-nuclear localization signal gene sequence, Golden Gate MoClo position CDS1
   

   Use

   Level 0 of moclo golden gate position cds1

   Available Since

   Aug. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGem-LP1-loxP-bar-lox2272-71

   Plasmid

   #168785

   Purpose

   Vector to create landing pad 1 (LP1) by homologous recombination in Aspergillus nidulans ΔSt site. The strain A. nidulans LP1 is used for recombinase mediated chromosomal integration.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

   Inserts

   bar
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2736011,2737053)
   Homology arm (ChrIV_A_nidulans_FGSC_A4:2790295,2791327)
   loxP
   lox2272-71
   

   Use

   Cre/Lox and Synthetic Biology

   Available Since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGem-LP2-loxP-bar-lox2272-71

    Plasmid

    #168786

    Purpose

    Vector to create landing pad 2 (LP2) by homologous recombination in Aspergillus nidulans IS1 site. The strain A. nidulans LP2 is used for recombinase mediated chromosomal integration.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

    Inserts

    bar
    IS1 Homology arm 1
    IS1 Homology arm 2
    loxP
    lox2272-71
    

    Use

    Cre/Lox and Synthetic Biology

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEM-Isce-Tol2-Tn5-neo

    Plasmid

    #176619

    Purpose

    for generating PCR templates with Tol2 repeats and Isce sites for BAC recombination

    Depositor

    Nathan Lawson

    Article

    Shih et al Development. 2021 Nov 9. pii: 273393. do

    Insert

    I-SceI sites, Tol2 repeats and Tn5-neo cassette
    

    Use

    Unspecified

    Available Since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEM-HE-bPAC(R278A)-Myc-eYFP

    Plasmid

    #165488

    Purpose

    Xenopus oocyte expression of soluble photoactivatable adenylyl cyclase derived from bPAC with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    bPAC(R278A)-Myc-eYFP
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    EYFP and Myc

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEM-myr-Citrine-SV40pA-FKF

    Plasmid

    #119865

    Purpose

    Donor cassette containing myr-tagged Citrine and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    myr-tagged Citrine and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    2x myristoylation

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMT-TPE2A-Mef2c-Tdtomato-Gata4-Tbx5

    Plasmid

    #111818

    Purpose

    Quadcistronic construct: Co-express four genes by three 2A peptides -- T2A, P2A and E2A

    Depositor

    Li Qian

    Article

    Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

    Inserts

    Mef2c (Mef2c Mouse)
    Tdtomato
    Gata4 (Gata4 Mouse)
    Tbx5
    

    Use

    Cloning intermediate

    Available Since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEM-HE-2xLyn-ERex-Venus-bPAC(F198Y)

    Plasmid

    #165489

    Purpose

    Xenopus oocyte expression of PACmn, a photoactivatable adenylyl cyclase derived from bPAC, membrane-anchored, no/reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    2xLyn-ERex-Venus-bPAC(F198Y)
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

    Plasmid

    #119864

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    HA-tagged biotin ligase (BirA), Thosea asigna vir…

    Expression

    Bacterial

    Available Since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid

    #140200

    Purpose

    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    dLbCas12a crRNA scaffold

    Promoter

    PgpdA

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRI012-pGEM-PacI-PU3-BsmbI-NotI

    Plasmid

    #140204

    Purpose

    Vector for cloning Cas9 sgRNA ( Step 1 of 2-step cloning). Contains AfU3 promoter driven sgRNA expression cassette flanked by PacI and NotI for further cloning in fungal vector.

    Depositor

    Yit Heng Chooi

    Article

    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology; Step 1 of cloning c…

    Promoter

    Aspergillus fumigatus U3 (RNAPIII).

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Expression

    Bacterial

    Available Since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEMHE:mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Plasmid

    #224755

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1 (S131C/C159A/C219A/C365A/C399A)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Mutation

    S131C/C159A/C219A/C365A/C399A

    Promoter

    T7

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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