We narrowed to 5,749 results for: actin

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pAAV CAG-DO-TC66T-mCherry

Plasmid

#175178

Purpose

Cre-off TVA-mCherry fusion protein with 66T mutation

Depositor

Bernardo Sabatini

Article

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

TVA

Use

AAV

Tags

mCherry

Mutation

E66T

Promoter

CAG

Available Since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

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PB CAG-R-3xSV40pA-R-td-sfGFP

Plasmid

#177150

Purpose

CAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)

Depositor

Mario Capecchi

Insert

Tandem dimer superfolder GFP

Use

Dre / rox ; piggybac

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-mDrc7-FLAG

Plasmid

#176470

Purpose

Expression vector of mouse dynein regulatory complex subunit 7 (Drc7) tagged with FLAG at C-terminus.

Depositor

Masahito Ikawa

Article

Morohoshi et al PLoS Genet. 2020 Jan 21;16(1):e1008585.

Insert

dynein regulatory complex subunit 7 (Drc7 Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CAG promoter

Available Since

Nov. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PB-PE VQR

Plasmid

#162797

Purpose

All-in-one prime editor piggyBac transposon, VQR variant

Depositor

Tobias Cantz

Article

Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

Insert

SpCas9_H840A_VQR-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)

Use

CRISPR and Synthetic Biology; Piggybac transposon

Tags

SV40 NLS

Expression

Mammalian

Mutation

D1135V, R1335Q, T1337R

Promoter

CAG

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-112–858_Flag2-Blast

Plasmid

#175039

Purpose

Mammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-1–729_Flag2-Blast

Plasmid

#175040

Purpose

Mammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein from amino acid positi…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8-∆ARD_Flag2-Blast

Plasmid

#175041

Purpose

Mammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Expression of MPP8 protein with deletion of the a…

Promoter

CAG

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pF3-CAG-Tol-FRT

Plasmid

#141349

Purpose

Shuttling cassette containing the Tol ORF

Depositor

Ton Schumacher

Article

Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

Insert

Tol cassette

Use

Mouse Targeting

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-SRGAP3FBAR-EGFP

Plasmid

#168506

Purpose

Expression of F-BAR domain (aa 1-492) of SRGAP3 with a C-terminal EGFP fusion

Depositor

Franck Polleux

Article

Coutinho-Budd et al J Cell Sci. 2012 Jul 15;125(Pt 14):3390-

Insert

SRGAP3-FBAR (SRGAP3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

aa 1-492

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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RGG-mCherry-RGG-eDHFR

Plasmid

#164648

Purpose

Two RGG domains tagged with mCherry and eDHFR

Depositor

Michael Lampson, Huaiying Zhang

Article

Zhang et al Mol Biol Cell. 2020 Aug 15;31(18):2048-2

Insert

RGG

Use

Cre/Lox

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP-mirNega

Plasmid

#163705

Purpose

pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

Depositor

Michisuke Yuzaki

Article

Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

Insert

mirNega and EGFP

Use

AAV

Promoter

CAG

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Venus-pA_U6-BbsI (pE057)

Plasmid

#149351

Purpose

Mammalian expression vector for Venus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

Venus

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-BFP-pA_U6-BbsI (pE058)

Plasmid

#149352

Purpose

Mammalian expression vector for BFP

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-miRFP670-pA_U6-BbsI (pE059)

Plasmid

#149353

Purpose

Mammalian expression vector for miRFP670

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

miRFP670

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1-NEO-CAG-BFP-pA-GFP-pA

Plasmid

#149344

Purpose

Vector for targeting the Replace reporter to the AAVS1 locus

Depositor

Ralf Kuehn

Article

Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

Insert

BFP

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pJM465 EF1α 3xFLAG-NLS-VP64-ZF1 in TUPV2

Plasmid

#161533

Purpose

Constitutive expression of 3xFLAG-NLS-VP64-ZF1 under the EF1α promoter

Depositor

Joshua Leonard

Article

Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

Insert

3xFLAG-NLS-VP64-ZF1

Use

Synthetic Biology

Tags

3x-FLAG

Expression

Mammalian

Promoter

EF1α

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAT-9222

Plasmid

#124222

Purpose

Cloning plasmid for EGxFP assay with self-targeting gRNA-s

Depositor

Ervin Welker

Article

Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

Insert

self-targeting gRNA between EGFP halves

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS_2pabPAC

Plasmid

#158968

Purpose

2P-activatable bPAC for transient expression

Depositor

Michiyuki Matsuda

Article

Kinjo et al ACS Chem Biol. 2020 Oct 19. doi: 10.1021

Insert

2pabPAC

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-TetR-RNF169UBD

Plasmid

#119011

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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