Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 125 results for: actin

Showing: 1 - 20 of 125 results

  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...LIfeAct-mCherry-N1 Actin Filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin Actin Filaments beta-actin EGFP ...Francois St-Pierre 158009 pCMV-mGold-Actin-C-18 Actin Filaments Actin mGold Francois St-Pierre 49149 mCh-alpha-tubulin...Wadsworth 27123 eTC GFP beta-actin full length Actin Filaments beta-actin EGFP Robert Singer 27382 pDEST...Vladislav Verkhusha 31949 pPAmCherry-b-actin Actin Filaments beta-actin PAmCherry Vladislav Verkhusha 79990...alpha-actinin 1 Actin Filaments alpha-actinin 1 EGFP Carol Otey 27676 Cortactin-pmCherryC1 Actin Filaments...ITPKA-mTurquoise2 Actin Filaments ITPKA mTurquoise2 Dorus Gadella 58473 pEGFP-C1 F-tractin-EGFP Actin Filaments...pLifeAct_mScarlet_N1 Actin Filaments LifeAct mScarlet Dorus Gadella 85055 pLifeAct_mScarlet-H_N1 Actin Filaments ...

  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...controls Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls...Western bot of liver lysate (10–40 µg) probed with β-actin (1:2000). Representative of three independent blots... Graphs showing the relative intensity of the β-actin (B) or total protein (C) on the membrane versus ...protein staining is a superior loading control to β-actin for Western blots,” pages 186–188, copyright 2013...difficult to see an increasing concentration of β-actin in panel A. Indeed, the quantification in panel ...-axes between panels B and C, indicating that β-actin is less sensitive as well as less linear than the...protein staining is a superior loading control to β-actin for Western blots. Analytical Biochemistry, 440(...

  3. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Expresses Nluc and copGFP from the chicken beta-actin CMV enhancer pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro 73040...promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ (Addgene...pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA Addgene Resources...

  4. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  ...Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive  Commonly used in expression...

  5. Allen Institute for Cell Science Plasmid Collection

    Type
    Collection
    ...Desmosomes 87425 ACTB-mEGFP AICSDP-15 mEGFP Beta-actin Actin filaments 87426 SEC61B-mEGFP AICSDP-7 mEGFP Sec61...Factor 124607 ACTN2-mEGFP AICSDP-63 mEGFP Alpha-actinin-2 Sarcomeric z-disks 124608 NPM1-mTagRFP-T AICSDP...

  6. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...cellular contraction. This network is composed of actin and myosin which interact with each other to generate...necessary for myosin activity and thus interaction with actin in nonmuscle systems in the fly. Spaghetti squash... an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti. We have two plasmids...7915669. Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430...

  7. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Micrographs of a fluorescently-labeled actin filament (488-Actin, cyan), with formin (649-mDia1, magenta...   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins in vitro reveals...mDia1 or 549-CP (upper, middle) and the effects on actin filament length (lower). d) Intensity and filament...Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping...became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR: The Virginia...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, ...endothelium, but they could be applied to study other actin-dependent processes in cells and tissues. And, this...

  9. MXS Chaining

    Type
    Blog Post
    ... Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ...detects a ubiquitously expressed protein, such as actin. Actin levels should be consistent across all samples...horseradish peroxidase (HRP), that emits light upon reacting with a specific substrate. The emitted light, ...

  13. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...use a tag? If you are fortunate enough to study actin, then you may not need to read this - but for many...

  14. Hot Plasmids - February 2022

    Type
    Blog Post
    ...4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization...
Showing: 1 - 20 of 125 results