We narrowed to 81 results for: actin

...pAAV-CAG-FLEX-rc [Jaws-KGC-tdTomato-ER2] AAV5, AAV8 Optogenetics Boyden New serotypes pAAV-CAG-tTA AAV2...repository since March.  Addgene is now offering an AAV2 CAG-driven tTA expresser from the Gradinaru lab. This... vitro and in vivo work with many cell types, as CAG is a ubiquitous and constitutive promoter. Use this...pAAV-Ef1a-fDIO-ChrimsonR-tdTomato AAV9 Controls Jensen New serotype pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA AAV9, PHP.eB Controls...

... 59462  AAV2  pAAV-CAG-tdTomato (codon diversified) 37825  AAV8*, AAV9*  pAAV-CAG-GFP 50465  AAV8*...Name 104496  AAV1  GP-AAV-CAG-FLEX-jGCaMP7f-WPRE AAV1 with a CAG promoter exhibits high specificity for...pAAV-FLEX-tdTomato pAAV-GFAP104-mCherry pAAV-mDlx-NLS-mRuby2 CAG-NLS-GFP pAAV-hSyn-mCherry Read on to learn more...pAAV-FLEX-tdTomato, pAAV-GFAP104-mCherry, pAAV-mDlx-NLS-mRuby2, CAG-NLS-GFP,and pAAV-hSyn-mCherry at Addgene. Viral vectors...

... Name Serotype Category Depositor  Notes AAV-CAG-jGCaMP8s-WPRE AAV1 Biosensor Looger New serotype ...AAV-CamKIIa-jGCaMP8m-WPRE AAV5 Biosensor Looger New serotype pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE AAV PHP.eB Biosensor Kim New serotype...AAV9 Biosensor St-Pierre New viral vector pAAV-CAG-RdLight1 AAV9 Biosensor Tian New viral vector pAAV-hSynapsin1....A184S AAV9 Biosensor Looger New serotype pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST AAV9 Optogenetics ...

...controls Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls...Western bot of liver lysate (10–40 µg) probed with β-actin (1:2000). Representative of three independent blots... Graphs showing the relative intensity of the β-actin (B) or total protein (C) on the membrane versus ...protein staining is a superior loading control to β-actin for Western blots,” pages 186–188, copyright 2013...difficult to see an increasing concentration of β-actin in panel A. Indeed, the quantification in panel ...-axes between panels B and C, indicating that β-actin is less sensitive as well as less linear than the...protein staining is a superior loading control to β-actin for Western blots. Analytical Biochemistry, 440(...

...pAAV.CMV.PI.EGFP.WPRE.bGH 37825  AAV1 pAAV-CAG-GFP 59462  AAV2 pAAV-CAG-tdTomato (codon diversified) 114472...AAV1, 2, 5, 8, 9 (20 µL size serotype testing) pAAV-CAG-GFP 50465 AAV8, 9 (20 µL size serotype testing)... Plasmid Serotype Name 104496  AAV1 pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 105322  AAV1 pGP-AAV-syn-FLEX-jGCaMP7c... 37825  AAVrg (20 µL size serotype testing) pAAV-CAG-GFP 50465 AAV1, 2, 5, rg (20 µL size serotype testing...

...pyogenes (Sp) Cas9 from a Cbh promoter. Cbh is a modified version of CAG promoter that the Zhang lab has tested...and most imporant is that, upstream of the Cbh promoter, this plasmid has a U6 promoter followed by BbsI...

...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  ... version is commonly used in promoter hybrids. CAG General expression mRNA Strong hybrid mammalian promoter...Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive  Commonly used in expression...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE (104493-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE (104495-AAVrg) pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (104496...serotypes. This collection is now complete! pAAV-CAG-GFP (plasmid 37825) and pAAV-hSyn-EGFP (plasmid 50465... AAV pCAG-FLEX-EGFP-WPRE (51502-AAV5): The CAG promoter directs ubiquitous expression. pAAV-mDlx-NLS-mRuby2...

...to AAV9. You can now target these cells with AAV-CAG-GFP packaged in AAV9-X1.1!  We also have eight new...Recombinases Jonathan Ting New viral prep pAAV-CAG-GFP AAV9-X1.1 Controls Edward Boyden New serotype...AAV5 Biosensors Loren Looger New serotype pAAV-CAG-FLEx-iSeroSnFR AAV9 Biosensors Lin Tian New viral...

...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: CAG promoter... promoter, FLEXed for expression in Cre-expressing cells hSynap: Human Synapsin-1 promoter. Good for neuronal...

...pGP-AAV-syn-FLEX-jGCaMP7b-WPRE 104496 AAVrg pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE 104495 AAVrg GP-AAV-CAG-FLEX-jGCaMP7s-WPRE 105714...potentials, is now offered under control of the CAG promoter for ubiquitous expression. (For more details on...

... Name Serotype Category Depositor  Notes pAAV-CAG-emiRFP670 AAV1 Control Kiryl Piatkevich New viral...Institute for Brain Science New viral tool pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE AAV5 Biosensor GENIE Project New...

...cellular contraction. This network is composed of actin and myosin which interact with each other to generate...necessary for myosin activity and thus interaction with actin in nonmuscle systems in the fly. Spaghetti squash... an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti. We have two plasmids...7915669. Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430...

...Micrographs of a fluorescently-labeled actin filament (488-Actin, cyan), with formin (649-mDia1, magenta...  Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins in vitro reveals...mDia1 or 549-CP (upper, middle) and the effects on actin filament length (lower). d) Intensity and filament...Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping...

...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, ...endothelium, but they could be applied to study other actin-dependent processes in cells and tissues. And, this...

...pAAV-hSyn-GRAB_OTmut AAV1 Biosensor Yulong Li New viral prep pAAV-CAG-NIR-GECO2G AAV1, AAV2, AAV9 Biosensor Robert Campbell...Karl Deisseroth New serotype (multiple) rAAV-CAG::FLEX-rev:: hM4D-2a-GFP AAV1 Chemogenetics Scott ...

...sensor Ace2N and additional GCaMP6 constructs: pAAV-CAG-dLight1.2 (AAV1) pGP-AAV-syn-FLEX-Ace2N-4AA-mNeon-ST...) pAAV-hSynapsin1-GCaMP6s-P2A-mRuby3 (AAV9) pAAV-CAG-SomaGCaMP6f2 (AAV9) Plus, additional AAV serotypes...pGP-AAV-syn-jGCaMP8f-WPRE (AAV Retrograde) pGP-AAV-CAG-FLEX-jGCaMP8m-WPRE (AAV Retrograde) pGP-AAV-syn-FLEX-jGCaMP8m-WPRE...

...example, the CAG promoter, a hybrid promoter consisting of a CMV enhancer, chicken beta actin promoter, and...

... Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin...

...AAVrg) alone at 1.7E6 viral genomes (vg)/cell, pAAV-CAG-FLEX-rc [Jaws-KGC-GFP-ER2] (Addgene 84445-AAVrg) ...Deisseroth (Addgene viral prep # 55632-AAVrg). pAAV-CAG- FLEX-rc [Jaws-KGC-GFP-ER2] was a gift from Edward...