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We narrowed to 30 results for: actin

Showing: 1 - 20 of 30 results
  1. Fluorescent Protein Guide: Subcellular Localization

    Type
    Collection
    ...LIfeAct-mCherry-N1 Actin filaments LifeAct mCherry Robin Shaw 21948 pCAG-mGFP-Actin Actin filaments beta-Actin EGFP ...pCMV-mGold2t-Actin-C-18 Actin filaments beta-Actin mGold2t Francois St-Pierre 231772 pCMV-mGold2s-Actin-C-18 ...18 Actin filaments beta-Actin mGold2s Francois St-Pierre 74259 pKanCMV-mClover3-18aa-actin Actin filaments...filaments beta-Actin mClover3 Michael Lin 74255 pKanCMV-mRuby3-18aa-actin Actin filaments beta-Actin mRuby3 Michael...Wadsworth 27123 eTC GFP beta-actin full length Actin filaments beta-Actin EGFP Robert Singer 40908 pDEST...Vladislav Verkhusha 31949 pPAmCherry-b-actin Actin filaments beta-Actin PAmCherry Vladislav Verkhusha 79990...-actinin 1 Actin filaments alpha-Actinin 1 EGFP Carol Otey 27676 Cortactin-pmCherryC1 Actin filaments ...
  2. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    ...controls Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls...Western bot of liver lysate (10–40 µg) probed with β-actin (1:2000). Representative of three independent blots... Graphs showing the relative intensity of the β-actin (B) or total protein (C) on the membrane versus ...protein staining is a superior loading control to β-actin for Western blots,” pages 186–188, copyright 2013...difficult to see an increasing concentration of β-actin in panel A. Indeed, the quantification in panel ...-axes between panels B and C, indicating that β-actin is less sensitive as well as less linear than the...protein staining is a superior loading control to β-actin for Western blots. Analytical Biochemistry, 440(...
  3. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    ...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  ...Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive  Commonly used in expression...
  4. Kazuhiro Oka Lentiviral Vectors

    Type
    Collection
    ...Expresses Nluc and copGFP from the chicken beta-actin CMV enhancer pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro 73040...promoter while pICPIS-CB contains the Chicken-beta actin promoter with CMV enhancer. pAdx-CMV-LacZ (Addgene...pICPIS-CB 73356 AdenoX shuttle vector with chicken-beta actin CMV enhancer promoter SV40 polyA Addgene Resources...
  5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    ...cellular contraction. This network is composed of actin and myosin which interact with each other to generate...necessary for myosin activity and thus interaction with actin in nonmuscle systems in the fly. Spaghetti squash... an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti. We have two plasmids...7915669. Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430...
  6. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ...Micrographs of a fluorescently-labeled actin filament (488-Actin, cyan), with formin (649-mDia1, magenta...  Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins in vitro reveals...mDia1 or 549-CP (upper, middle) and the effects on actin filament length (lower). d) Intensity and filament...Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping...
  7. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, ...endothelium, but they could be applied to study other actin-dependent processes in cells and tissues. And, this...
  8. MXS Chaining

    Type
    Blog Post
    ... Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin...
  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    ...use a tag? If you are fortunate enough to study actin, then you may not need to read this - but for many...
  10. Hot Plasmids - February 2022

    Type
    Blog Post
    ...4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization...
  11. Hot Plasmids: Summer 2025

    Type
    Blog Post
    ... Figure 1: Super-resolution imaging of actin filaments with mGold2. Top: TIRF-SIM imaging of ...
Showing: 1 - 20 of 30 results