We narrowed to 30 results for: actin

Find plasmids encoding fluorescent fusion proteins with known subcellular localization patterns for colocalization studies or organelle labeling.

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

Addgene's conference rooms are named after gene names. Learn more about these genes including sonic hedgehog, sleeping beauty, groucho, and cookie monster.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

Lentiviral Vectors deposited by Kazuhiro Oka from the Baylor College of Medicine Gene Vector Core

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

The Allen Institute for Cell Science has deposited plasmids to generate fluorescently tagged proteins of key cellular structures in human cell lines.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

An overview of the western blotting process from separating proteins by size, transfer, staining, and visualizing.

The Michael Davidson lab deposited their collection of 3,300 fluorescent protein plasmids including empty backbones to Addgene.

A review of the advantages of epitope tags and commonly used tags and their antibodies.

Our February hot plasmids, including two new CRISPR tools, a protein ladder, and anti-FLAG frankenbodies.

R bodies are tiny, tunable molecule machines that can be used to break membranes. Learn all about them and their potential uses here.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

We provide high quality AAV preps from select plasmids in the repository. Browse the University of Florida serotype testing panel for the eye and brain.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.