We narrowed to 24 results for: actin

This post covers loading controls and normalization of semi-quantitative western blots. We cover housekeeping proteins and total protein loading controls.

April 8, 2025, 1:15 p.m.

Addgene's conference rooms are named after gene names. Learn more about these genes including sonic hedgehog, sleeping beauty, groucho, and cookie monster.

April 2, 2019, 1:44 p.m.

The next post in the Plasmids 101 series focuses on the promoter region, the element responsible for initiating the transcription of your insert into RNA.

April 3, 2014, 8:05 p.m.

Ready to let your tags do the work for you? Learn about self-labelling protein tags and how they can give you flexibility in your experimental approach!

April 16, 2024, 1:15 p.m.

Optogenetics aren't just for neuroscientists! In this post, we review optogenetic tools for cytoskeleton and membrane control in cells.

Sept. 28, 2023, 1:15 p.m.

Learn how you can use MXS-Chaining to generate complex, multicistronic plasmid constructs.

Feb. 7, 2017, 3:30 p.m.

An overview of the western blotting process from separating proteins by size, transfer, staining, and visualizing.

March 8, 2021, 2:41 p.m.

The Michael Davidson lab deposited their collection of 3,300 fluorescent protein plasmids including empty backbones to Addgene.

July 29, 2014, 6:16 p.m.

A review of the advantages of epitope tags and commonly used tags and their antibodies.

May 11, 2023, 1:15 p.m.

Our February hot plasmids, including two new CRISPR tools, a protein ladder, and anti-FLAG frankenbodies.

Feb. 22, 2022, 2:15 p.m.

R bodies are tiny, tunable molecule machines that can be used to break membranes. Learn all about them and their potential uses here.

April 14, 2016, 2:30 p.m.

Learn how RANbodies provide a scaffold for new, easy to detect, nanobodies.

April 10, 2018, 12:56 p.m.

Learn how you can visualize newly synthesized proteins with FUNCAT-PLA.

Jan. 16, 2018, 3:20 p.m.

All new plasmids deposited at Addgene are verified by next-generation sequencing to provide you with more data to choose the right plasmid for your next experiment.

April 6, 2021, 12:30 p.m.

Check out the latest Hot Plasmids, viral vectors, and antibodies in the Addgene repository!

Aug. 28, 2025, 1:15 p.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

Expansion microscopy allows for light microscope imaging of fixed tissues while overcoming the 250 nm resolution limit.

June 20, 2023, 1:15 p.m.

Learn about some things to consider when designing your fusions - where to make the fusions, the size and shape of the protein, and linker size.

April 9, 2019, 1:13 p.m.

Looking for a way to study integrins? IPI's first deposit, a toolset comprised of anti-integrin antibodies, is now available in Addgene's repository!

March 28, 2024, 1:15 p.m.

Everything you need to know about antibodies in this blog post! Get the facts about the types of antibodies and how they can be used in the lab.

Jan. 19, 2021, 2:15 p.m.