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We narrowed to 104 results for: actin

Showing: 1 - 20 of 104 results

  1. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type
    Blog Post
    Published
    April 8, 2025, 1:15 p.m.
    ...controls Ubiquitously expressed proteins like β-actin or GAPDH are popular choices for loading controls...Western bot of liver lysate (10–40 µg) probed with β-actin (1:2000). Representative of three independent blots... Graphs showing the relative intensity of the β-actin (B) or total protein (C) on the membrane versus ...protein staining is a superior loading control to β-actin for Western blots,” pages 186–188, copyright 2013...difficult to see an increasing concentration of β-actin in panel A. Indeed, the quantification in panel ...-axes between panels B and C, indicating that β-actin is less sensitive as well as less linear than the...protein staining is a superior loading control to β-actin for Western blots. Analytical Biochemistry, 440(...

  2. Plasmids 101: The Promoter Region – Let's Go!

    Type
    Blog Post
    Published
    April 3, 2014, 8:05 p.m.
    ...ubiquitous. human beta actin General expression mRNA Mammalian promoter from beta actin gene Constitutive  ...Constitutive  Contains CMV enhancer, chicken beta actin promoter, and rabbit beta-globin splice acceptor...expression mRNA Strong insect promoter from Drosophila Actin 5c gene Constitutive  Commonly used in expression...

  3. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...cellular contraction. This network is composed of actin and myosin which interact with each other to generate...necessary for myosin activity and thus interaction with actin in nonmuscle systems in the fly. Spaghetti squash... an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti. We have two plasmids...7915669. Dominguez, Roberto, and Kenneth C. Holmes. "Actin structure and function." (2011). PubMed PMID: 21314430...

  4. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    Published
    April 16, 2024, 1:15 p.m.
    ...Micrographs of a fluorescently-labeled actin filament (488-Actin, cyan), with formin (649-mDia1, magenta...   Figure 3: Single-molecule tracking of actin filament assembly-regulating proteins in vitro reveals...mDia1 or 549-CP (upper, middle) and the effects on actin filament length (lower). d) Intensity and filament...Shekhar, S. (2023). Multicomponent regulation of actin barbed end assembly by twinfilin, formin and capping...became known as SNAP-tag. There is also a fast-reacting version of SNAP-tag called SNAPf. eDHFR: The Virginia...

  5. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...most-important component of the cytoskeleton – actin? Actin dynamics control cell morphology, movement, ...endothelium, but they could be applied to study other actin-dependent processes in cells and tissues. And, this...

  6. MXS Chaining

    Type
    Blog Post
    Published
    Feb. 7, 2017, 3:30 p.m.
    ... Membranes 3 mCherry 587nm/ 610 nm human β-Actin Actin 4 Citrine 516nm/ 529nm human α-Tubulin Tubulin...

  7. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...detects a ubiquitously expressed protein, such as actin. Actin levels should be consistent across all samples...horseradish peroxidase (HRP), that emits light upon reacting with a specific substrate. The emitted light, ...

  9. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...use a tag? If you are fortunate enough to study actin, then you may not need to read this - but for many...

  10. Visualizing Protein Turnover In Situ

    Type
    Blog Post
    Published
    Jan. 16, 2018, 3:20 p.m.
    ...Adina R., Bin Wu, and Robert H. Singer. "Single β-actin mRNA detection in neurons reveals a mechanism for...

  11. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ...4x-FLAG-mCherry-H2B), cytoskeletal (middle, 4xFLAG-mCherry-β-actin), and mitochondrial (mito-mCherry-1xFLAG) localization...

  13. RANbodies: Reporter Nanobody Fusions

    Type
    Blog Post
    Published
    April 10, 2018, 12:56 p.m.
    ...: GFP, RFP,  the histone protein H2A2B, and the actin-binding protein gelsolin, but any nanobody sequence...

  15. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...doesn’t mean we should start re-conceptualizing actin filaments or microtubules. This issue is shared ...

  16. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...plasmids, or promoters such as the chicken beta-actin promoter of the pHLsec vector. In this scenario,...

  17. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...mechanical forces in the cell’s microenvironment. The actin cytoskeleton exerts tensile force, transmitted through...
Showing: 1 - 20 of 104 results