Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,720 results for: donor

Clear keywords
Showing: 4181 - 4200 of 4720 results

  1. pSCL.177

    Plasmid
    #184998
    Purpose
    Express -Eco1 EMX1 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: EMX1 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    EMX1 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184998-plasmid-map-sequence-id-376623

    Enlarge

  2. pSCL.175

    Plasmid
    #184996
    Purpose
    Express -Eco1 HEK3 editing ncRNA and gRNA
    Depositor
    Seth Shipman
    Article
    Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d
    Insert
    Eco1: HEK3 targeting and editing ncRNA-gRNA (a1/a2 length: 27 v1), expressed constitutively from a pol III (H1) promoter
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    HEK3 donor, a1/a2 length extended for 27 bp
    Promoter
    H1
    Available Since
    Nov. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184996-plasmid-map-sequence-id-376621

    Enlarge

  3. pSA091 PSMD14_IVS2-Cas9-BFP

    Plasmid
    #249141
    Purpose
    Overexpression of SpCas9-BFP with PSMD14 IVS2 intron in 5' UTR and blasticidin resistance gene. Contains Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    PSMD14_IVS2-Cas9-TagBFP (PSMD14 Human, S. pyogenes Cas9, synthetic)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    PSMD14 IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 8, 2026
    Availability
    Academic Institutions and Nonprofits only
    249141-plasmid-map-sequence-id-496524

    Enlarge

  4. pPD687 A2UCOE-HBB_IVS2-EnAsCas12a-BFP

    Plasmid
    #249160
    Purpose
    Overexpression of EnAsCas12a-BFP with HBB IVS2 intron in 5' UTR with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.
    Depositor
    Michael Bassik
    Article
    Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi
    Insert
    A2UCOE-HBB_IVS2-EnAsCas12a-TagBFP (HBB Synthetic, Human)
    Use
    CRISPR; Cp36 donor dna
    Expression
    Mammalian
    Mutation
    HBB IVS2 intron
    Promoter
    EF1a
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    249160-plasmid-map-sequence-id-496435

    Enlarge

  5. R777-E033 Hs.DUSP1

    Plasmid
    #70317
    Purpose
    Gateway ORF clone of human DUSP1 [NM_004417.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP1 (DUSP1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70317-plasmid-map-sequence-id-125766

    Enlarge

  6. Hs.NRAS Q61K

    Plasmid
    #83180
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61K
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83180-plasmid-map-sequence-id-158143

    Enlarge

  7. Hs.NRAS Q61R

    Plasmid
    #83179
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61R mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61R
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83179-plasmid-map-sequence-id-158142

    Enlarge

  8. pODN-AAVS1-mR2-CAAX

    Plasmid
    #183871
    Purpose
    Repair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Article
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Insert
    AAVS1 homology arms with CMV-driven mRuby2-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mRuby2-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available Since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    183871-plasmid-map-sequence-id-373475

    Enlarge

  9. R777-E039 Hs.DUSP4

    Plasmid
    #70323
    Purpose
    Gateway ORF clone of human DUSP4 [NM_001394.6] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP4 (DUSP4 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70323-plasmid-map-sequence-id-125772

    Enlarge

  10. R777-E227 Hs.RASA1

    Plasmid
    #70511
    Purpose
    Gateway ORF clone of human RASA1 [NM_002890.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASA1 (RASA1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70511-plasmid-map-sequence-id-125960

    Enlarge

  12. pMH015_AAVS1_puro-pA-core-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179437
    Purpose
    Dual fluorescent reporter construct with double core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    coreHS4-TRE-cit-coreHS4-mch (DC)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179437-plasmid-map-sequence-id-355553

    Enlarge

  13. pMH012_AAVS1_puro-pA-9xTetO-pEF-H2B-mCitrine-core-pRSV-H2B-mCherry

    Plasmid
    #179435
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, arms for integration at AAVS1 locus
    Depositor
    Lacra Bintu
    Article
    Lensch et al Elife. 2022 Jun 9;11. pii: 75115. doi: 1
    Insert
    TRE-cit-coreHS4-mch (SC)
    Expression
    Mammalian
    Promoter
    pEF alpha, pRSV
    Available Since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    179435-plasmid-map-sequence-id-355551

    Enlarge

  14. R777-E015 Hs.BRAF

    Plasmid
    #70299
    Purpose
    Gateway ORF clone of human BRAF [NM_004333.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    BRAF (BRAF Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70299-plasmid-map-sequence-id-125748

    Enlarge

  15. Hs.KRAS4b Q61H

    Plasmid
    #83140
    Purpose
    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), Q61H mutation
    Depositor
    Dominic Esposito
    Insert
    KRAS (KRAS Human)
    Use
    Gateway Cloning
    Mutation
    Q61H
    Available Since
    Sept. 13, 2016
    Availability
    Academic Institutions and Nonprofits only
    83140-plasmid-map-sequence-id-158103

    Enlarge

  16. R777-E135 Hs.MRAS

    Plasmid
    #70419
    Purpose
    Gateway ORF clone of human MRAS [NM_001085049.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MRAS (MRAS Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70419-plasmid-map-sequence-id-125868

    Enlarge

  17. R777-E035 Hs.DUSP2

    Plasmid
    #70319
    Purpose
    Gateway ORF clone of human DUSP2 [NM_004418.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP2 (DUSP2 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70319-plasmid-map-sequence-id-125768

    Enlarge

  18. R777-E125 Hs.MAPK1

    Plasmid
    #70409
    Purpose
    Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MAPK1 (MAPK1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70409-plasmid-map-sequence-id-125858

    Enlarge

  19. R777-E243 Hs.RASGRP1

    Plasmid
    #70527
    Purpose
    Gateway ORF clone of human RASGRP1 [NM_005739.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASGRP1 (RASGRP1 Human)
    Use
    Gateway Cloning
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70527-plasmid-map-sequence-id-125976

    Enlarge

  20. pMini-CMV-NLS-dead R-IscB-NLS_T2A_mCherry_U1a-EGFP trans-splicing ωRNA

    Plasmid
    #246436
    Purpose
    Expresses R-IscB with EGFP-repairing ωRNA in mammalian cells for trans-splicing assessments.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    O.gue IscB with dead mutations (D60A, H269A) and delta-TID
    mCherry
    ωRNA with trans-splicing template
    Tags
    GFP N-terminal sequence (M1 to Q95), Hemi Intron …
    Expression
    Mammalian
    Mutation
    GFP N-terminal sequence M1 to Glutamine 95 append…
    Promoter
    CMV and U1a
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246436-plasmid-map-sequence-id-490914

    Enlarge

Showing: 4181 - 4200 of 4720 results