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We narrowed to 4,705 results for: golden gate

Showing: 4181 - 4200 of 4705 results
  1. TL1 alphaDdCBE A1 (DddA6-C) - TL1 alphaL_DdCBE 2 (left, DddA11-C)

    Plasmid
    #221994
    Purpose
    Expresses mitochondrial cytosine base editor arm in mammalian cells
    Depositor
    Insert
    COX8A MTS-3xFLAG-TALE NT-alphaN-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    TALE: Q231R, W232G, S233A | DddA: T1413I
    Promoter
    CMV
    Available Since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pTE5418_pMBP-GlcR

    Plasmid
    #221192
    Purpose
    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.
    Depositor
    Insert
    glcR (pden4400 from Paracoccus denitrificans)
    Tags
    10x His tag with E. coli maltose binding protein …
    Expression
    Bacterial
    Promoter
    T7 promoter and T7 promoter-lacO
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  3. pTE5419_p3WJdB-glcO36

    Plasmid
    #221193
    Purpose
    GlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminator
    Depositor
    Insert
    T7 promoter-glcO36
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    T7 promoter with downstream glcO36 operator
    Available Since
    Sept. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  4. pSCL475

    Plasmid
    #219503
    Purpose
    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans
    Depositor
    Insert
    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    Expression
    Yeast
    Promoter
    GAL7
    Available Since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
  5. pEvolvR-mCherry-NNG-Slug-nCas9(D10A)-PolI5MΔ

    Plasmid
    #249079
    Purpose
    Expresses nucleus-localized NNG-Slug-nCas9 (D10A) fused to PolI5MΔ and mCherry using a CMV promoter. Includes a GFP cassette flanked by BsmbI cut sites to knock in and coexpress user-defined gRNAs.
    Insert
    NNG-Slug-nCas9-PolI5MΔ
    Use
    CRISPR
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    deletion of PolI5M N-terminal flap endonuclease d…
    Promoter
    CMV
    Available Since
    Jan. 7, 2026
    Availability
    Academic Institutions and Nonprofits only
Showing: 4181 - 4200 of 4705 results