We narrowed to 14,894 results for: pET

1. pETDuet1_NIX(1-182aa)-THROMBIN-GST (W36A/L39A; deltaLIR)

   Plasmid

   #223738

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   NIX (BNIP3L) soluble part (BNIP3L Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   W36A/L39A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pETDuet1_FUNDC1(1-50aa)-THROMBIN-GST (Y18A/L21A; deltaLIR)

   Plasmid

   #223739

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   FUNDC1 soluble part, mutant (FUNDC1 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   Y18A/L21A; deletion of LIR

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETDuet1_BCL2L13(1-465aa)-THROMBIN-GST (W276A/I279A; deltaLIR1)

   Plasmid

   #223749

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   BCL2L13 soluble part (BCL2L13 Human)
   

   Use

   Tags

   Thrombin cleavage-GST

   Expression

   Bacterial

   Mutation

   expresses aa 1-465, with W276A/I279A; deletion of…

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

   Plasmid

   #223724

   Purpose

   Expression of recombinant protein for purification

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   FIP200-CTR (RB1CC1 Human)
   

   Use

   Tags

   6xHis-TEV-EGFP

   Expression

   Bacterial

   Mutation

   aa 1429-1591 only

   Promoter

   Available since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28b-SUMO-FLAG-TeKaiB-1-94-Y8A-Y94A-FLAG

   Plasmid

   #229223

   Purpose

   For expression of T. elongatus KaiB protein mutant

   Depositor

   Andy LiWang

   Insert

   kaiB (kaiB T. elongatus)
   

   Use

   Tags

   FLAG and SUMO-FLAG

   Expression

   Bacterial

   Mutation

   1-94 (truncated), Y8A, Y94A

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAM316 pET28a-GGG-Titin[V15P]-23-K-9-C-14cyclinB-10GS-Y

   Plasmid

   #226349

   Purpose

   Expression of Titin V15P substrate with GS tail

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   Available since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

   Plasmid

   #169135

   Purpose

   E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Coh7
   

   Use

   Tags

   3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET01-TAF5-ex8-Minigene-SRSF1 motif MUT

   Plasmid

   #218973

   Purpose

   Vector for constitutive expression of TAF5 alternative exon-8 mini gene with mutated SRSF1 motif (AGTCTCCT)

   Depositor

   Thomas Gonatopoulos-Pournatzis

   Article

   Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

   Insert

   TAF5 exon-8 (with mutant SRSF1 motif )and the native flanking intronic sequences spanning the exon-8
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutant SRSF1 binding motif (AGTCTCCT)

   Promoter

   RSV LTR

   Available since

   June 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-RPL3-191~307-mEGFP(A206K)-TwStrep

   Plasmid

   #208626

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa191~307, fused with mEGFP(A206K) and Twin Strep tag

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) aa 191~307 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   only aa 191~307, other residues not included

   Promoter

   T7

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-RPL3-299~398-mEGFP(A206K)-TwStrep

    Plasmid

    #208627

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa299~398, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 299~398 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 299~398, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-RPL3-212~285-mEGFP(A206K)-TwStrep

    Plasmid

    #208629

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa212~285, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 212~285 (RPL3 Human)
    

    Use

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 212~285, other residues not included

    Promoter

    T7

    Available since

    Jan. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETM-60-NcQDE2 _506-938-del787-838-GGSG_O

    Plasmid

    #147085

    Purpose

    Bacterial Expression of NcQde-2_506-938-del787-838

    Depositor

    Elisa Izaurralde

    Insert

    NcQde-2_506-938-del787-838
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pETM-60-NcQDE2 _506-938-del787-838-GSG_O

    Plasmid

    #147086

    Purpose

    Bacterial Expression of NcQde-2_506-938-del787-838

    Depositor

    Elisa Izaurralde

    Insert

    NcQde-2_506-938-del787-838
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Plasmid

    #170788

    Purpose

    Expresses pET-15b(+) Mortierella verticillate (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Mortierella verticillate PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid

    #170789

    Purpose

    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Toxoplasma gondii PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lacI

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Plasmid

    #166422

    Purpose

    Express pET-15b(+) Plasmodium falciparum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Insert

    Plasmodium falciparum PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

    Plasmid

    #166423

    Purpose

    Express pET-15b(+) Pythium oligandrum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Pythium oligandrum PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

    Plasmid

    #166424

    Purpose

    Express pET-15b(+) Reticulomyxa filosa PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Reticulomyxa filosa PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

    Plasmid

    #166425

    Purpose

    Express pET-15b(+) Sphaeforma arctica PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Sphaeforma arctica PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Plasmid

    #166415

    Purpose

    Express pET-15b(+) Acytostelium subglobosum PRL (Δprenylation motif)

    Depositor

    Kalle Gehring

    Article

    Fakih et al J Biol Chem. 2023 Apr;299(4):103055. doi

    Insert

    Acytostelium subglobosum PRL
    

    Use

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Mutation

    Deleted nucleotides coding for 4 C-terminal amino…

    Promoter

    AmpR, lac1

    Available since

    Sept. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge