We narrowed to 14,172 results for: cas9

Showing: 4201 - 4220 of 14172 results

Results per page:

Sort results by:

pPB-R1R2_EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112924

Purpose

piggyBac transposon vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Piggybac transposon vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A695Q

Plasmid

#207368

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A695Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A926Q

Plasmid

#207370

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A926Q

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-E526K

Plasmid

#207365

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-E526K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-V495M

Plasmid

#207363

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-V495M

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Y515N, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A1060R

Plasmid

#207372

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1060R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, K1003A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A1003K

Plasmid

#207371

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A1003K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

Promoter

CBh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A848K

Plasmid

#207369

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A848K

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HeFSpCas9-A661R

Plasmid

#207367

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-HeFSpCas9-A661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-Q661R

Plasmid

#207366

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-Q661R

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, Y515N, K526E, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-evoSpCas9-N515Y

Plasmid

#207364

Purpose

Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9-N515Y

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

M495V, K526E, R661Q, amino acids 1005-1013 replac…

Promoter

CBh

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
HypaR-SpCas9

Plasmid

#126757

Purpose

Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

Hypa2SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A, N692A, M694A, Q695A, H698A

Promoter

CBh

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
B-HypaR-SpCas9

Plasmid

#126764

Purpose

Expression plasmid for human codon-opt. increased fidelity Blackjack-Hypa2SpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than Hypa2SpCas9.

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Hypa2SpCas9

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

R661A; N692A; M694A; Q695A; H698A; amino acids 10…

Promoter

CBh

Available Since

Sept. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR EGFP-guide3

Plasmid

#118164

Purpose

CRISPRi negative control. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter, and sgRNA3 against the EGFP CDS

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

KRAB-dCas9-2A-BlastR

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hPGK and U6

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Plasmid

#207863

Purpose

AAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNA

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
v3em-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Plasmid

#207861

Purpose

AAV genome encoding C-terminal PE6c and U6 expression cassettes for Rosa26 twinPE pegRNA pairs

Depositor

David Liu

Article

Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

Insert

NpuC-Cterm-PE6c-dualU6-Rosa26-twinPE-attB

Use

AAV

Mutation

See Manuscript

Promoter

Cbh

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-BE4max-NRCH

Plasmid

#136920

Purpose

Expresses BE4max-NRCH in mammalian cells

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

BE4max-NRCH

Use

CRISPR

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

Plasmid

#114072

Purpose

T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag

Tags

NLS(nucleoplasmin) and 6xHis

Expression

Bacterial

Mutation

E174R, S542R, and K548R

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-EF1alpha-dCas9-VP64-MS2-VP64-Blast

Plasmid

#192666

Purpose

3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A Blast resistance marker

Depositor

Jeffrey Miner

Insert

dCas9-VP64, MS2-VP64

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A in Cas9

Promoter

EF1a

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
P3-dCas9-Tet1-GFP-Puro

Plasmid

#190728

Purpose

Expression of human spdCas9-Tet1 fusion for targeted DNA methylation in mammalian cell. EGFP-puromycin double marker under an independent CMV promoter. Cloning backbone for spgRNA (BbsI)

Depositor

Maja Jagodic

Article

Pahlevan Kakhki et al Nat Commun. 2024 Jul 30;15(1):6419. doi:

Insert

dCas9-tet1

Use

CRISPR

Tags

3XFLAG and SV40 NLS

Expression

Mammalian

Mutation

Changed Pro434 codon from CCG to CCC and Gly468 f…

Promoter

pCAG

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge