We narrowed to 579 results for: hyper

1. pLKO.1B CDC25A_1

   Plasmid

   #160767

   Purpose

   Suppress CDC25A

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   shCDC25A_1
   

   Use

   Lentiviral

   Available Since

   Oct. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pABE8e-NG-403Q-Nterm-AAV

   Plasmid

   #194651

   Purpose

   N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG N-term
   

   Use

   AAV

   Tags

   BPNLS and NpuN spilt intein

   Promoter

   TNNT2

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pABE8e-NG-403Q-Cterm-AAV

   Plasmid

   #194652

   Purpose

   C-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

   Depositor

   David Liu

   Article

   Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

   Insert

   ABE8e-NG C-term
   

   Use

   AAV

   Tags

   BPNLS and NpuC split intein

   Promoter

   TNNT2

   Available Since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. MOM603

   Plasmid

   #133242

   Purpose

   expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

   Depositor

   Shinsuke Niwa

   Article

   Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

   Insert

   kif1a (KIF1A Human)
   

   Tags

   StrepII tag and mScarlet

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

   Plasmid

   #154787

   Purpose

   Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain

   Depositor

   Robert Singer

   Article

   Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

   Insert

   Adenosine deaminse (Adar Fly, Synthetic)
   

   Use

   Lentiviral

   Tags

   GFP, V5, and stdMCP

   Expression

   Mammalian

   Mutation

   Synonymized serine to prevent autoediting of ADAR…

   Promoter

   UBC

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-Blast POLA1

   Plasmid

   #160807

   Purpose

   Express POLA1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   POLA1 (POLA1 Human)
   

   Use

   Retroviral

   Mutation

   Codon Optimized

   Available Since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCW57.1 DOX off blast NFS1

   Plasmid

   #160801

   Purpose

   Express Dox repressible NFS1

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   NFS1 (NFS1 Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXS-IRES-Blast HA-POLE2

   Plasmid

   #160809

   Purpose

   Express HA-tagged POLE2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   HA-POLE2 (POLE2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Available Since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pShuttle CMV RGECO-TnT

   Plasmid

   #124643

   Purpose

   Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination.

   Depositor

   Matthew Daniels, Paul Robinson, Hugh Watkins

   Article

   Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

   Insert

   RGECO-TnT (TNNT2 Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. CaV1.3e[8a,11,31b,Δ32,Δ42a]

    Plasmid

    #49332

    Purpose

    Repaired Addgene #26577 to agree with the rat ref sequence. Repairs are @: nt 6224 (GCC->GTC) [Ala->Val]; nt 3310 (GTG->GCG) [Val->Ala]; nt 731 (TCA->GGA) [Ser->Gly].

    Depositor

    Diane Lipscombe

    Article

    Xu et al J Neurosci. 2001 Aug 15. 21(16):5944-51.

    Insert

    CACNA1D (Cacna1d Rat)
    

    Expression

    Mammalian

    Mutation

    Contains exons 8a, 11, and 31b.Exons 32 and 42a a…

    Promoter

    CMV

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENTR-3xFLAG-YAP1-S127A

    Plasmid

    #42239

    Depositor

    William Pu

    Article

    von Gise et al Proc Natl Acad Sci U S A. 2012 Feb 14;10

    Insert

    YAP1 (YAP1 Human)
    

    Use

    Gateway

    Tags

    3xFLAG

    Mutation

    Serine 127 changed to alanine

    Promoter

    none

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac1-BacMam-4x-PyOtR

    Plasmid

    #217364

    Purpose

    Expresses four copies pyrrolysyl tRNA variant "PyOtR" with U6 promoter; can be packaged in bacmid

    Depositor

    Abhishek Chatterjee

    Article

    Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

    Insert

    4 copies of pyrrolysyl-tRNA mutant PyOtR
    

    Use

    Baculoviral

    Expression

    Insect and Mammalian

    Promoter

    U6

    Available Since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-C1 EDD

    Plasmid

    #37190

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Tags

    6x His Tag and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSG5 EDD

    Plasmid

    #37191

    Depositor

    Darren Saunders, Charles Watts

    Article

    Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

    Insert

    EDD (UBR5 Human)
    

    Tags

    6x His Tag and FLAG

    Expression

    Mammalian

    Mutation

    K503R (please see depositor comments below)

    Promoter

    SV40

    Available Since

    Aug. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-HA-mBec1

    Plasmid

    #99505

    Purpose

    3rd generation lentiviral expression of wild-type Becn1

    Depositor

    Congcong He

    Article

    Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

    Insert

    Beclin 1 (Becn1 Mouse)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    CMV

    Available Since

    Aug. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pESC-LEU-HsAIDSc

    Plasmid

    #60810

    Purpose

    to express human AID recoded for yeast codon usage

    Depositor

    Thomas Kunkel, Youri Pavlov

    Article

    Mayorov et al BMC Immunol. 2005 Jun 10;6:10.

    Insert

    AID (AICDA Human)
    

    Tags

    Myc

    Expression

    Yeast

    Mutation

    codon optimized for yeast

    Promoter

    GAL1

    Available Since

    Dec. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGlomyc-Klf4

    Plasmid

    #172870

    Purpose

    Mammalian expression vector with myc-tagged mouse Klf4 coding sequence

    Depositor

    Renee van Amerongen

    Article

    Mourao et al bioRxiv 2021.06.22.449461

    Insert

    Klf4 (Klf4 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pShuttle CMV RGECO-TnI

    Plasmid

    #124642

    Purpose

    Myofilament localised red genetically encoded calcium sensor in mammalian expression vector used for AdEasy adenoviral DNA recombination

    Depositor

    Matthew Daniels, Paul Robinson, Hugh Watkins

    Article

    Sparrow et al Circ Res. 2019 Feb 8. doi: 10.1161/CIRCR

    Insert

    RGECO-TnI (TNNI3 Synthetic, Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. UCP3 promoter EP1

    Plasmid

    #71743

    Purpose

    Contains 7kb of the murine UCP3 gene encompassing the core promoter, exon 1, intron 1, and part of exon 2; used to quantify promoter activity

    Depositor

    Heinrich Taegtmeyer

    Article

    Harmancey et al J Biol Chem. 2015 Nov 10. pii: jbc.M115.

    Insert

    Uncoupling Protein 3 promoter (Ucp3 Mouse)
    

    Tags

    Luciferase

    Expression

    Bacterial

    Mutation

    Contains 7kb of the murine UCP3 gene encompassing…

    Promoter

    UCP3

    Available Since

    Jan. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Tet-pLKO-puro shPOLE1_1

    Plasmid

    #160810

    Purpose

    Express Dox repressible shPOLE1

    Depositor

    Richard Possemato

    Article

    Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

    Insert

    shPOLE1_1
    

    Use

    Lentiviral

    Available Since

    Jan. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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