We narrowed to 1,919 results for: rpe

1. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV UBC msNLRP3(WT)-APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #218633

   Purpose

   Proximity biotinylation of msNLRP3

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Lentiviral

   Tags

   APEX2-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   UBC

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV UBC APEX2-3xFLAG-IRES-eGFP

   Plasmid

   #214983

   Purpose

   Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   APEX2 (APX2 Glycine max (soybean))
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mutation

   Promoter

   UBC

   Available since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-N-Flag-HA-GFP

   Plasmid

   #41034

   Purpose

   Retroviral expression of GFP in mammalian cells

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   GFP
   

   Use

   Retroviral; Gateway destination vector

   Tags

   Flag, HA, and IRES Puro

   Expression

   Mammalian

   Mutation

   Promoter

   LTR-driven expression

   Available since

   Oct. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pJT11F

   Plasmid

   #165062

   Purpose

   Introducing GAL promoters-controlled monterpene limonene-producing module and Y-FAST and in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   T-RPL3>ERG20-N127W>P-GAL1-P-GAL10>Y-FAST>T-ALD6-P-GAL2>ClLS>T-RPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. msHook2 g1 lentiCRISPRv2-mCherry

   Plasmid

   #218652

   Purpose

   Knockout vector for mouse Hook2

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Hook2 (Hook2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221 msNLRP3 (STOP)

   Plasmid

   #218638

   Purpose

   Gateway shuttling vector for cloning N-terminal NLRP3 tags (contains a stop codon--note: bulky N-terminal tags are likely not functional)

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   NLRP3 (Nlrp3 Mouse)
   

   Use

   Gateway entry

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. msHook2 g2 lentiCRISPRv2-mCherry

   Plasmid

   #218653

   Purpose

   Knockout vector for mouse Hook2

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   Hook2 (Hook2 Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJT9RFR

   Plasmid

   #165067

   Purpose

   Introducing GAL promoters-controlled sequiterpene nerolidol-producing module and Y-FAST and in S. cerevisiae

   Depositor

   Claudia Vickers

   Article

   Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

   Insert

   T-RPL3>ERG20>P-GAL1-P-GAL2>Y-FAST>ErB1.2A-AcNES1>T-RPL41B
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   WT

   Promoter

   Available since

   March 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZP47 Nlk1

    Plasmid

    #16895

    Depositor

    Randall Moon

    Article

    Thorpe et al Development. 2004 Jun;131(12):2899-909.

    Insert

    Nlk1 (nlk1 Zebrafish)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. pMA4008

    Plasmid

    #70109

    Purpose

    mtDNA destruction/production of rho-0 cells

    Depositor

    Mikhail Alexeyev

    Article

    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi

    Inserts

    EGFP
    Herpes simplex virus UL12.5M185
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    N-terminal deletion of UL12.5

    Promoter

    CMV and RSV

    Available since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTargeT gE

    Plasmid

    #60845

    Purpose

    Expression of the varicella zoster glycoprotein (gE) in mammalian cells

    Depositor

    Charles Grose

    Article

    Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

    Insert

    ORF68 (ORF68 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pHAGE-FLAG-APEX2-GFP

    Plasmid

    #175757

    Purpose

    Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

    Depositor

    Wade Harper

    Article

    Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEC2735 (pSpy0C2)

    Plasmid

    #220279

    Purpose

    integrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombination

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hTMEM115-TEV-3xHA-P2A-mScarlet HDR template

    Plasmid

    #214998

    Purpose

    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mScarlet-I for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    hTMEM115-TEV-3xHA-P2A-mScarlet HDR template (TMEM115 Human)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template

    Plasmid

    #214999

    Purpose

    Endogenously tag human TMEM115 with a C-terminal 3xHA tag in addition to cytosolic mNG for cell sorting.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    hTMEM115-TEV-3xHA-P2A-mNeonGreen HDR template (TMEM115 Human)
    

    Use

    Hdr template

    Tags

    3xHA

    Expression

    Mutation

    Promoter

    None

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE pGK hNLRP3-mNG

    Plasmid

    #218643

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak pGK promoter with blast selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDONR221 hNLRP3-mNG-STOP

    Plasmid

    #218644

    Purpose

    Gateway vector for shuttling NLRP3 C-terminally tagged with mNeonGreen to different destination vectors (start and stop codon)

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Gateway entry

    Tags

    mNeonGreen

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pH6Z-806

    Plasmid

    #85255

    Purpose

    Plasmid clone containing HHV-6B(Z29) restriction endonuclease fragment

    Depositor

    Philip Pellett

    Article

    Lindquester et al Arch Virol. 1996;141(2):367-79.

    Insert

    fragment of the human herpesvirus 6B strain Z29 genome
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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