We narrowed to 5,457 results for: golden gate
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Plasmid#238335PurposeproUBI10::VIH2-FL::FLAG-H741A ubiquitous expression in Arabidopsis plantsDepositorAvailable sinceJune 27, 2025AvailabilityAcademic Institutions and Nonprofits only
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pKS1334
Plasmid#239508PurposeExpress CDT2 membrane protein cargo, peroxisomal Ynd1p truncation, and Pex22 peroxisomal chauffeurDepositorInsertNcCDT2-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable sinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLII-CRISPR for CKA4
Plasmid#238521PurposePlant expression of Cas9 and gRNAs against A. thaliana CK2alpha4DepositorAvailable sinceJune 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSG1017-2xBsaI-SaKKH-2xUGI_P2A_EGFP
Plasmid#239450PurposeCodon-optimized S. aureus KKH Cas9 with 2x UGI. 2x BsaI sites for easy cloning of varying deaminases for cytosine base editing. EGFP can serve as a transfection marker.DepositorTypeEmpty backboneUseCRISPRTagsEGFPExpressionMammalianPromoterCMVAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSG1016-2xBsaI-SaKKH_P2A_EGFP
Plasmid#239461PurposeCodon-optimized S. aureus KKH Cas9. 2x BsaI sites for easy cloning of varying deaminases. EGFP can serve as a transfection marker.DepositorTypeEmpty backboneUseCRISPRTagsEGFPExpressionMammalianPromoterCMVAvailable sinceJune 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF_AaRaf1/Raf2/RbcX2/BSD2
Plasmid#229507PurposeExpresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX2,BSD2DepositorPublicationInsertsRubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX2
BSD2
ExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDF_AaRaf1/Raf2/RbcX1/BSD2
Plasmid#229508PurposeExpresses Anthoceros agrestis chaperones: Raf1,Raf2,RbcX1,BSD2DepositorPublicationInsertsRubisco accumulation factor 1
Rubisco accumulation factor 2
RbcX1
BSD2
ExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available sinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgPlxnb2-2 in pT4-U6-sgRNA-CMV-EGFP
Plasmid#239589Purposeexpresses guide#2 to boost the expression of mouse Plxnb2, via CRISPRaDepositorInsertsgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)
UseSleeping beauty (sb) transposonExpressionMammalianPromoterhU6Available sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgPlxnb2-3 in pT4-U6-sgRNA-CMV-EGFP
Plasmid#239590Purposeexpresses guide#3 to boost the expression of mouse Plxnb2, via CRISPRaDepositorInsertsgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)
UseSleeping beauty (sb) transposonExpressionMammalianPromoterhU6Available sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJJB112
Plasmid#239493PurposeExpress peroxisomal VenusDepositorInsertUbi-R-Venus-ePTS1
ExpressionYeastAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pVL1172
Plasmid#239535PurposeExpress CpPT1 membrane protein cargo with C-terminal mTurqoise2 marker but without any targeting sequence and Venus ER markerDepositorInsertCpPT1(52-408)-mTurquoise2
ExpressionYeastAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPSmF
Plasmid#226912PurposeMinimal plasmid for Plant STARR-seq with enhancer candidates in forward orientationDepositorTypeEmpty backboneExpressionPlantAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPSmR
Plasmid#226913PurposeMinimal plasmid for Plant STARR-seq with enhancer candidates in reverse orientationDepositorTypeEmpty backboneExpressionPlantAvailable sinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF5x1-mRuby2-miRE.FF5-bGH
Plasmid#235259PurposeComMAND closed-loop circuit regulating mRuby2 with miRE-FF5 design 3DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available sinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-TS.FF6x1-mRuby2-miRE.FF5-bGH
Plasmid#235258PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF5 design 3DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available sinceJune 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1619
Plasmid#239550PurposeExpress GAL-inducible cytochrome P450 reductase tagged with mTagBFP2 and Pex22 peroxisomal chauffeur; chromosomal integration by CRISPRDepositorInsertCrCPR-mTagBFP2-2xCrk_SH3
ExpressionYeastAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgPlxnb2-1 in pT4-U6-sgRNA-CMV-EGFP
Plasmid#239588Purposeexpresses guide#1 to boost the expression of mouse Plxnb2, via CRISPRaDepositorInsertsgRNA targeting TSS-upstream reagion of Plxnb2 (Plxnb2 Mouse)
UseSleeping beauty (sb) transposonExpressionMammalianPromoterhU6Available sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1140
Plasmid#239501PurposeExpress GAL-inducible peroxisomal sfCherry3C large fragment and Pex22 peroxisomal chauffeur with 2 high affinity SH3 peptidesDepositorInsertsfCherry3C(1-10)-ePTS1
ExpressionYeastAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1170
Plasmid#239504PurposeExpress GAL-inducible cytosolic sfCherry3C large fragment and Pex22 peroxisomal chauffeur with medium affinity SH3 peptideDepositorInsertsfCherry3C(1-10)-dPTS1
ExpressionYeastAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKS1577
Plasmid#239525PurposeExpress Vba2p membrane protein cargo, peroxisomal Ynd1p truncation, and inactive Pex22 peroxisomal chauffeur as negative controlDepositorInsertScVba2p-mTurquoise2-2xCrk_SH3
ExpressionYeastAvailable sinceJune 12, 2025AvailabilityAcademic Institutions and Nonprofits only