We narrowed to 5,125 results for: golden gate

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pAIP4_2D5J

Plasmid

#234143

Purpose

Heterologous protein expression of unsaturated glucuronyl hydrolase in Escherichia coli

Depositor

Christopher Bahl

Insert

2D5J

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_1EDG

Plasmid

#234144

Purpose

Heterologous protein expression of GUNA_RUMCH in Escherichia coli

Depositor

Christopher Bahl

Insert

1EDG

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_2GAS

Plasmid

#234145

Purpose

Heterologous protein expression of isoflavone reductase (IFR) in Escherichia coli

Depositor

Christopher Bahl

Insert

2GAS

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAIP4_1H6T

Plasmid

#234146

Purpose

Heterologous protein expression of internalin B (InlB) in Escherichia coli

Depositor

Christopher Bahl

Insert

1H6T (inlB Listeria monocytogenes)

Tags

10x HisTag-Smt3

Expression

Bacterial

Promoter

T7

Available Since

March 6, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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B8-pVTCB052

Plasmid

#231302

Purpose

Plasmid used for expression of AI-designed GFP protein B8 in E. coli

Depositor

Alexander Rives

Article

Hayes et al Science. 2025 Feb 21;387(6736):850-858.

Insert

B8

Expression

Bacterial

Available Since

March 3, 2025

Availability

Academic Institutions and Nonprofits only

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Gal4-pcVP16-1

Plasmid

#231570

Purpose

Expresses the single component, light-off regulated pcVP16-1 transcriptional regulator

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Gal4-pcVP16-1

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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Zdk3-VPR

Plasmid

#231572

Purpose

Expresses components of the LOOMINA optogenetic transcriptional control system.

Depositor

Dominik Niopek

Article

Muench et al Nucleic Acids Res. 2024 Dec 16:gkae1237.

Insert

Zdk3-VPR, Ef1a driven

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3342 PB-PEmax-puro

Plasmid

#226674

Purpose

PiggyBac cargo vector encoding PEmax and puromycin resistance.

Depositor

Chang Liu

Article

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Insert

PEmax

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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Human Coxsackievirus A16 strain G10 inactive 2A protease

Plasmid

#228665

Purpose

Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Enterovirus A71 2A protease

Tags

His-SUMO

Expression

Bacterial

Mutation

Avitag insertion and C2A mutations / Active site …

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228659

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Wesselsbron virus portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228657

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

SLEV portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228656

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Spondweni virus portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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ROCV strain SPH 34675 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228655

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Rocio virus for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Rocio virus portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PAF23

Plasmid

#223171

Purpose

Expresses yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

green fluorescent protein

Expression

Yeast

Promoter

Saccharomyces cerevisiae CTR1

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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PAF24

Plasmid

#223172

Purpose

Expresses VC83_00191-yeGFP under control of CTR1 promoter and terminator

Depositor

Ryan Peterson

Article

Anne et al J Fungi (Basel). 2024 Oct 21;10(10):729.

Insert

Copper Transporter

Tags

StreptagII and yeGFP

Expression

Yeast

Promoter

saccharomyces cerevisiae CTR1

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228652

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 4 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DENV-3 strain Phillippines/H87/1956 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228651

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 3 for biochemical assays

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 3 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker

Plasmid

#228648

Purpose

Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assay

Depositor

ASAP Discovery, Frank von Delft

Insert

Dengue 1 portion of NS2B protease fused to NS3

Tags

His-Twin Strep-Tev

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Echovirus 11 (E11 3C) protease A isolate E11-PLA-NECK-23-E0-62-P1-FRA23

Plasmid

#228645

Purpose

Bacterial expression of the E11 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Echo virus 11 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012

Plasmid

#228640

Purpose

Bacterial expression of the Coxsackievirus 43 isoform B 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Coxsackievirus 43 isoform B 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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