We narrowed to 4,636 results for: pcr

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Clim-DC2-lifeact-mCherry

Plasmid

#129558

Purpose

pClimDCLifeAct:mCh actin filaments marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

lifeAct fused to mCherry

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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DHC1-mIAA17 Hygro

Plasmid

#140544

Purpose

DHC1 tagging with mIAA7

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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IgH P+ VA20

Plasmid

#127249

Purpose

IgH V-region

Depositor

Christine Milcarek

Article

Shell et al J Immunol. 2007 Dec 1;179(11):7663-73.

Insert

Promoter+V-region of IgH of A20 B cell line

Use

Other

Promoter

IgH

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA GPR137

Plasmid

#226809

Purpose

Gateway entry vector for GPR137

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

GPR137 (GPR137 Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_001164351

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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OPRL1-DuET

Plasmid

#213360

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

OPRL1 (OPRL1 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRF1 CTF-FLAG

Plasmid

#217694

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF1 (ADGRF1 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-566

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED 4CA-FLAG

Plasmid

#199681

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED 4CA-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED 4CA (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

C595.604.629.638 of LYCHOS mutated to A

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a LZ LED Y551A-FLAG

Plasmid

#199680

Purpose

Bacterial expression of Leucine Zipper (LZ) LYCHOS LED Y551A-Flag

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

Leucine zipper LYCHOS LED Y551A (GPR155 Human)

Tags

6xHis and Flag

Expression

Bacterial

Mutation

Y551 of LYCHOS mutated to A

Promoter

T7 promoter

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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Clim-DC2-H2B2-Venus

Plasmid

#129556

Purpose

pClimDC-H2B:mV nuclear marker

Depositor

Inaki Ruiz-Trillo

Article

Kozyczkowska et al Curr Biol. 2021 Sep 27;31(18):4104-4110.

Insert

Histone 2B fused to venus

Expression

Bacterial

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pZA9

Plasmid

#158487

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

no STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA1

Plasmid

#158479

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible wild-type A. thaliana ZAR1 with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLG1-puro-sgATL1-2

Plasmid

#109006

Purpose

CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

ATL1-sgRNA #2 (ATL1 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

mU6 promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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NK816

Plasmid

#257341

Purpose

Encodes the 3’ end of S. rosetta PTSG_03655 CDS (without STOP codon) fused to a linker and followed by mStayGold CDS and the 3’ UTR region of S. rosetta PTSG_03655.

Depositor

Nicole King

Article

De Las Bayonas et al bioRxiv 2026.06.27.734982

Insert

PTSG_03655_3'CDS-recovery-sequence-linker-mStayGold-PTSG_03655-3’UTR

Tags

mStayGold

Expression

Bacterial

Mutation

Synonymous codon mutations made to PTSG_03655 3&#…

Available Since

June 30, 2026

Availability

Academic Institutions and Nonprofits only

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pENTR1A 2xHA HCAR3

Plasmid

#226807

Purpose

Gateway entry vector for 2xHA HCAR3

Depositor

Ramiro Iglesias-Bartolome

Article

Pedro et al J Invest Dermatol. 2024 Jun;144(6):1311-

Insert

HCAR3 (HCAR3 Human)

Use

Gateway Cloning

Tags

2xHA tag followed by a GDPPVAT linker

Mutation

Sequence optimized from NP_006009.2

Available Since

Jan. 7, 2025

Availability

Academic Institutions and Nonprofits only

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DHC1-mIAA7 Neo

Plasmid

#140543

Purpose

DHC1 tagging with mIAA7

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

DHC1 (DYNC1H1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pZA3

Plasmid

#158481

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, STOP codon

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pZA2

Plasmid

#158480

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA11

Plasmid

#158489

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 L17E without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E no STOP codon

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pZA4

Plasmid

#158482

Purpose

Entry clone for Golden Gate assembly. Golden Gate compatible A. thaliana ZAR1 L17E/D489V with STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

L17E, D489V, STOP codon

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pZA10

Plasmid

#158488

Purpose

Entry clone for Golden Gate assembly. Golden gate compatible A. thaliana ZAR1 D489V without STOP codon

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

ZAR1 (ZAR1 Mustard Weed)

Use

Golden gate entry vector

Mutation

D489V, no STOP codon

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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