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tdminiSOG-PCNA-19

Plasmid

#57806

Purpose

Localization: Nucleus, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

PCNA (PCNA Human)

Tags

tdminiSOG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
ReaLiSM A1-Hinge-GPIba+2cys

Plasmid

#59581

Purpose

express VWF A1-GPIba ReaLiSM construct in mammalian cells

Depositor

Timothy Springer

Publication

Kim et al Nature. 2010 Aug 19;466(7309):992-5. doi

Insert

VWF A1-linker-GPIba (VWF Human)

Tags

6His

Expression

Mammalian

Promoter

chick beta-actin

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-CAG2-GCaMP6s

Plasmid

#241022

Purpose

An AAV transfer plasmid expressing the GCaMP6s genetically encoded calcium indicator under control of the ubiquitously expressed CAG2 promoter.

Depositor

Thomas Johnson

Publication

Aguzzi et al Sci Transl Med. 2026 Apr 29;18(847):eadr

Insert

GCaMP6s

Use

AAV

Tags

6X His tag

Promoter

CAG2

Available since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1alpha-DEST-IRES-Neo

Plasmid

#253766

Purpose

Third generation lentiviral expression Gateway cloning destination vector under a human EF1alpha promoter for expression of a gene of interest linked to neomycin selection by an IRES.

Depositor

Samuel McBrayer

Publication

Savani et al Nat Metab. 2026 Apr 29. doi: 10.1038/s42

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

April 22, 2026

Availability

Academic Institutions and Nonprofits only

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pLKO.1 hygro shRNA human Fis1

Plasmid

#252272

Purpose

Knockdown of human Fis1

Depositor

David Chan

Publication

Nakajima et al Curr Biol. 2026 Mar 9;36(5):1205-1219.e8

Insert

shRNA human Fis1 (FIS1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Feb. 27, 2026

Availability

Academic Institutions and Nonprofits only

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pAT1089-(PB-4_EF1α-Blast_2A_rtTA3 [HA-NLS-dCas9-NLS])

Plasmid

#251108

Purpose

Expression of dCas9 component of Casilio-i system

Depositor

Chia-Lin Wei

Publication

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

dCas9

Tags

HA

Expression

Mammalian

Mutation

N/A

Available since

Feb. 10, 2026

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-GFP-WPRE-bGHpA

Plasmid

#231018

Purpose

For strong lentivirus-mediated expression of GFP

Depositor

Tomasz Nowakowski

Publication

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Enhanced GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shDrp1-GFP

Plasmid

#228737

Purpose

GFP expressing shRNA targeting Drp1

Depositor

Cagla Eroglu

Publication

Rodriguez Salazar et al J Cell Biol. 2025 Oct 6;224(10):e2024101

Insert

Drp1 (Dnm1l, Dnm1l Mouse, Rat)

Expression

Mammalian

Available since

Dec. 3, 2025

Availability

Academic Institutions and Nonprofits only

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AAV-CMV-Vcan-V3-HA

Plasmid

#238076

Purpose

Expresses Vcan V3 in mice heart tissues

Depositor

Yuxuan Guo

Publication

Bai et al Cell Prolif. 2025 Jun 3:e70070. doi: 10.

Insert

Vcan (Vcan Mouse)

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR_BIP-sfGFP-TurboID-KDEL

Plasmid

#240223

Purpose

Gateway entry clone with ER-localized TurboID tagged with superfolder GFP (contains stop codon)

Depositor

Justin Bosch, Norbert Perrimon

Publication

Bosch et al Nat Commun. 2026 Apr 20. doi: 10.1038/s4

Insert

BIP-sfGFP-TurboID-KDEL

Use

Gateway shuttle vector

Available since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mTagBFP2-P2A-NeoR

Plasmid

#239568

Purpose

Cytosolic expression of mTagBFP2

Depositor

Tim Ahfeldt, Joel Blanchard

Publication

et al iScience 28 (2025)

Insert

mTagBFP2

Use

Lentiviral

Promoter

UbC

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pFUW mEmerald-P2A-NeoR

Plasmid

#239569

Purpose

Cytosolic expression of mEmerald

Depositor

Tim Ahfeldt, Joel Blanchard

Publication

et al iScience 28 (2025)

Insert

mEmerald

Use

Lentiviral

Promoter

UbC

Available since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-MCP-SRSF3-V5-mCherry

Plasmid

#235090

Purpose

SRSF3 mCherry fusion protein with MCP domain

Depositor

Mitchell Guttman

Publication

Bhat et al Nature. 2024 May;629(8014):1165-1173. do

Insert

pCAG-MCP-SRSF3-V5-mCherry (SRSF3 Human)

Expression

Mammalian

Promoter

CAG

Available since

May 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMRX-IZ-SEC61B-mCherry-FRB

Plasmid

#233349

Purpose

Stable expression of SEC61B-mCherry-FRB in mammalian cells. FRB localizes in the ER lumen.

Depositor

Noboru Mizushima

Publication

Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.

Inserts

SEC61B (SEC61B Human)
mCherry
FRB

Use

Retroviral

Expression

Mammalian

Mutation

T2098L mutant of FKBP-rapamycin binding domain of…

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

Plasmid

#232127

Purpose

A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

Depositor

Joshua Wythe

Publication

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

GATA1 (GATA1 Human)

Use

Gateway Cloning

Tags

V5

Available since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pML346_VPS35-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227891

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Publication

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

VPS35 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML374_CLTA-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227893

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Publication

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CLTA homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML307_SEC61B-N-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227894

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Publication

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

SEC61B homology arms with mEGFP-3xHA for insertion at N terminus

Use

CRISPR

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pML383_CCDC47-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227896

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Publication

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

CCDC47 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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mActA-FKBP-mCherry

Plasmid

#210295

Purpose

ActuAtor for FKBP-FRB CID

Depositor

Takanari Inoue

Publication

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

mActA-FKBP-mCherry

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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