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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 14,607 results for: pET

Showing: 4701 - 4720 of 14607 results

  1. pUB-MSP1D1

    Plasmid
    #161609
    Purpose
    Bacterial expression of mutated membrane scaffold protein ("ultra-bright" MSP1D1) for Nanodisc formation
    Depositor
    Stephen Sligar
    Article
    McLean et al Anal Biochem. 2020 Oct 15;607:113860. do
    Insert
    UD-MSP1D1
    Use
    Tags
    7-His tag
    Expression
    Bacterial
    Mutation
    mutant of MSP1D1 ( Q138W, L181W, Y236W)
    Promoter
    T7
    Available since
    Nov. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    161609-plasmid-map-sequence-id-317666

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  4. pAGD289

    Plasmid
    #228113
    Purpose
    pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter
    Depositor
    Seth Shipman
    Article
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Kva1 retron, ncRNA with 90 bp rpoB donor
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228113-plasmid-map-sequence-id-455374

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  11. pCoofy48

    Plasmid
    #55186
    Purpose
    Bacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and GST tags. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    6x His, GST, His10-Stop-OneStrep-Stop-S-tag-Stop-…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 26, 2014
    Availability
    Academic Institutions and Nonprofits only
    55186-plasmid-map-sequence-id-464535

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  12. pHIT179

    Plasmid
    #204510
    Purpose
    Bacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assays
    Depositor
    Yuji Kohara
    Article
    Aoki et al EMBO J. 2007 Dec 12. 26(24):5007-19.
    Insert
    csr-1a (C. elegans) (csr-1 Nematode)
    Use
    Tags
    6xHis, MBP
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    204510-plasmid-map-sequence-id-403822

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  15. pMH-macroHC

    Plasmid
    #82337
    Purpose
    Provides a C-terminal macro domain and 6xHis tag for expression in E. coli
    Depositor
    Michael Hothorn
    Article
    Wild et al Protein Sci. 2016 Oct 24. doi: 10.1002/p
    Insert
    SPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
    Use
    Tags
    6xHis non-cleavable and macro domain of human his…
    Expression
    Bacterial
    Mutation
    Truncated protein, amino acids 1-178
    Promoter
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    82337-plasmid-map-sequence-id-155830

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  18. pZF-BCB-GCN

    Plasmid
    #127476
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-GCN
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    Use
    Cell-free transcription–translation
    Tags
    GCN4 leucine zipper
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    127476-plasmid-map-sequence-id-248524

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  19. pPD3C

    Plasmid
    #86971
    Purpose
    Expresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038
    Insert
    PCNA3 fused to phosphite dehydrogenase and P450cam
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    E175A/A176R in PTDH, N106R in PCNA3, silent mutat…
    Promoter
    T7 promoter
    Available since
    March 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    86971-plasmid-map-sequence-id-173928

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  20. pZF-ADD-GCN

    Plasmid
    #127475
    Purpose
    coding sequence for synthetic zinc finger ZF-ADD-GCN
    Depositor
    Sebastian Maerkl
    Article
    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11
    Insert
    ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
    Use
    Cell-free transcription–translation
    Tags
    GCN4 leucine zipper
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127475-plasmid-map-sequence-id-248906

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Showing: 4701 - 4720 of 14607 results