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We narrowed to 6,501 results for: nls

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Showing: 4861 - 4880 of 6501 results

  1. pYPQAT27

    Plasmid
    #223399
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223399-plasmid-map-sequence-id-452308

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  2. pTX208

    Plasmid
    #89268
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02
    Depositor
    Daniel Voytas
    Publication
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsYSA-gRNA01 and OsYSA-gRNA02
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Promoter
    Maize Ubiquitin1 promoter
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89268-plasmid-map-sequence-id-177893

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  3. AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)

    Plasmid
    #190112
    Purpose
    AAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFP
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.
    Inserts
    MMLV-RT(dRH)
    U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
    Use
    AAV
    Tags
    bpNLS and bpNLS-P2A-eGFP
    Mutation
    mutations from RT in PE2 and truncation of RNAse …
    Promoter
    EFS and U6, H1
    Available since
    Sept. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    190112-plasmid-map-sequence-id-372717

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  5. pCAG-T3-eSpCAS9-NG-pA

    Plasmid
    #131466
    Purpose
    Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys
    Depositor
    Wataru Fujii
    Publication
    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized eSpCas9-NG
    Use
    CRISPR
    Tags
    Flag, NLS, and polyA
    Expression
    Mammalian
    Available since
    Nov. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    131466-plasmid-map-sequence-id-262445

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  7. pYPQ230-STU

    Plasmid
    #138110
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized LbCas12a without promoter and terminator
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    LbCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138110-plasmid-map-sequence-id-272040

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  8. pcDNA-Flag-LmoCas8b2

    Plasmid
    #126482
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas8b2
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126482-plasmid-map-sequence-id-246628

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  9. pcDNA-Flag-LmoCas7

    Plasmid
    #126483
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas7
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126483-plasmid-map-sequence-id-246629

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  10. pcDNA-Flag-LmoCas5

    Plasmid
    #126484
    Purpose
    Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter
    Depositor
    Charles Gersbach
    Publication
    Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LmoCas5
    Use
    CRISPR
    Tags
    Flag, NLS
    Expression
    Mammalian
    Promoter
    CMV and U6
    Available since
    Sept. 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    126484-plasmid-map-sequence-id-246630

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  19. pVG122

    Plasmid
    #211765
    Purpose
    Expresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.
    Depositor
    Sahand Jamal Rahi
    Publication
    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:
    Insert
    strongLOV
    Use
    For single-copy integration (if cut with pmei)
    Tags
    SV40 NLS and VP16
    Expression
    Yeast
    Mutation
    compared to EL222, strongLOV contains Glu84Asp mu…
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    211765-plasmid-map-sequence-id-417945

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Showing: 4861 - 4880 of 6501 results