Targeted transcriptional modulation with type I CRISPR-Cas systems in human cells.
Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, Beisel CL, Barrangou R, Gersbach CA
Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 10.1038/s41587-019-0235-7. PubMed Article
Pickar-Oliver A, Black JB, Lewis MM, Mutchnick KJ, Klann TS, Gilcrest KA, Sitton MJ, Nelson CE, Barrera A, Bartelt LC, Reddy TE, Beisel CL, Barrangou R, Gersbach CA
Nat Biotechnol. 2019 Sep 23. pii: 10.1038/s41587-019-0235-7. doi: 10.1038/s41587-019-0235-7. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 106270 | pcDNA-Flag-EcoCas8e | Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 106271 | pcDNA-Flag-EcoCse2 | Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 106272 | pcDNA-Flag-EcoCas7 | Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 106273 | pcDNA-Flag-EcoCas5 | Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 106274 | pcDNA-Flag-EcoCas6 | Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 106275 | pcDNA-CasE-p300-Flag | Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of E tag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter |
| 106276 | pAPcrRNA_Eco | E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter |
| 126481 | pEcoCascade-IL1RNcrRNA | Encodes E.coli CRISPR Type I-E crRNA targeting IL1RN |
| 126482 | pcDNA-Flag-LmoCas8b2 | Encodes L.monocytogenes CRISPR Type I-B Cas8b2 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 126483 | pcDNA-Flag-LmoCas7 | Encodes L.monocytogenes CRISPR Type I-B Cas7 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 126484 | pcDNA-Flag-LmoCas5 | Encodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 126485 | pcDNA-Flag-LmoCas6 | Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter |
| 126486 | pcDNA-LmoCas6-p300 | Encodes L.monocytogenes CRISPR Type I-B Cas6 with N-terminal fusion of Flag epitope and NLS and C-terminal fusion of NLS, human p300 HAT core (aa1048-1664), and Flag tag epitope driven by CMV promoter |
| 126487 | pLmoCascade-IL1RNcrRNA | Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN |
| 126488 | pLenti-LmoCas8b2 | Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas8b2 driven by hUbC promoter |
| 126489 | pLenti-LmoCas7 | Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas7 driven by hUbC promoter |
| 126490 | pLenti-LmoCas5 | Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas5 driven by hUbC promoter |
| 126491 | pLenti-LmoCas6 | Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6 driven by hUbC promoter |
| 126492 | pLenti-LmoCas6-KRAB-2a-BlastR | Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter |
| 126493 | pLenti-LmoCascade-IL1RNcrRNA | Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting IL1RN |
| 126494 | pLenti-LmoCascade-HBE1crRNA | Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B crRNA targeting HBE1 |
| 126501 | pAPcrRNA_Lmo | L.monocytogenes CRISPR Type I-B crRNA cloning vector for mammalian expression driven by U6 promoter |