We narrowed to 5,457 results for: golden gate

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pPOM034_P7_3"Lys3

Plasmid

#216459

Purpose

3' homology arm for S. pombe genomic integration. Part type 7 following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

3''lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-hU6-crCoV-Mutiplex_EF1a-BFP

Plasmid

#224788

Purpose

SARS-COV-2 targeting crRNA array for RfxCas13d expressed from single hU6 promoter and reporter BFP protein expressed from EF1a promoter

Depositor

Gabor Balazsi

Publication

Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

Insert

crCoV20, crCoV21, crCoV24

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

hU6 and hU6-2xTetO

Available since

Oct. 31, 2024

Availability

Academic Institutions and Nonprofits only

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FUS-miRFP670-TRF1

Plasmid

#227378

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, WT (TERF1 Human)

Use

Lentiviral

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMulti_A_ccdB

Plasmid

#223851

Purpose

GEC-acceptor for 4G-cloning, designed for expression in E. coli (Ampicillin resistance)

Depositor

Stephan Gruber

Publication

Taschner et al Life Sci Alliance. 2024 Dec 2;8(1):e2024

Type

Empty backbone

Use

Golden-gate acceptor

Expression

Bacterial

Available since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pPOM038_P8b_5'Lys3

Plasmid

#216463

Purpose

5' homology arm for S. pombe genomic integration. Part type 8b following the YeastToolkit MoClo grammar.

Depositor

Tomáš Pluskal

Publication

Hebra et al ACS Synth Biol. 2023 Nov 22. doi: 10.102

Insert

5'lys3

Use

Synthetic Biology

Expression

Yeast

Mutation

Removal of any BsaI // BsmBI restriction site

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pTBL2368 MTK PB puro backbone

Plasmid

#226673

Purpose

Empty MTK PB cargo backbone marked with puromycin resistance.

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Type

Empty backbone

Expression

Mammalian

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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DVK_pBR322_FG

Plasmid

#217595

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries F-G CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Publication

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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DVK_pBR322_AF

Plasmid

#217593

Purpose

Destination vector with kanamycin resistance and pBR322 origin of replication. Carries A-F CIDAR MoClo overhangs for level 1 construct cloning.

Depositor

Wei Huang

Publication

Nielsen et al ACS Synth Biol. 2025 Jan 12. doi: 10.102

Type

Empty backbone

Use

Synthetic Biology

Mutation

None

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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HNRNPA1-miRFP670-TRF1

Plasmid

#227384

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric HNRNPA1 IDR (TERF1, HNRNPA1 Human)

Use

Lentiviral

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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iLID-miRFP670-TRF1

Plasmid

#227385

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric iLID (TERF1 Human)

Use

Lentiviral

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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FUS-15YS-miRFP670-TRF1

Plasmid

#227382

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 15YS (TERF1 Human)

Use

Lentiviral

Mutation

15YS

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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FUS-5YS-miRFP670-TRF1

Plasmid

#227380

Purpose

Adhesion module, creates 'seed' at telomere

Depositor

Clifford Brangwynne

Publication

Strom et al Cell. 2024 Aug 14:S0092-8674(24)00828-6.

Insert

Telomeric FUS IDR, 5YS (TERF1 Human)

Use

Lentiviral

Mutation

5YS

Available since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJB67

Plasmid

#226316

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with K to A CsoS2 MR1-6

Expression

Bacterial

Mutation

K261A, K320A, K380A, K436A, K495A, K546A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB68

Plasmid

#226315

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1-7

Expression

Bacterial

Mutation

Y300A, Y359A, Y420A, Y475A, Y534A, Y585A, Y644A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB74

Plasmid

#226314

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1-5

Expression

Bacterial

Mutation

Y300A, Y359A, Y420A, Y475A, Y534A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB73

Plasmid

#226313

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1-3

Expression

Bacterial

Mutation

Y300A, Y359A, Y420A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB72

Plasmid

#226312

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with Y to A CsoS2 MR1

Expression

Bacterial

Mutation

Y300A

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB50

Plasmid

#226311

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-7

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB55

Plasmid

#226310

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-5

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJB53

Plasmid

#226309

Purpose

E. coli and H. neapolitanus shuttle vector; integrates the gene of interest and a kanamycin resistance marker into a neutral site on the H. neapolitanus genome.

Depositor

David Savage

Publication

Turnsek et al J Biol Chem. 2024 Jul 4;300(8):107532. d

Insert

CsoS2 with V(T/S)G to AAA CsoS2 MR1-3

Expression

Bacterial

Mutation

VTG273-5AAA, VTG284-6AAA, VTG295-7AAA, VSG332-4AA…

Promoter

pTRC

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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