We narrowed to 5,545 results for: donor

1. pSLQ6332 - ACTB CRISPRa CLIP Donor

   Plasmid

   #210015

   Purpose

   CLIP donor for integrating CRISPRa (dCas12-miniVPR)-T2A-GFP-P2A to the ACTB locus

   Depositor

   Stanley Qi

   Article

   Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

   Insert

   dLbCpf1-miniVPR-T2A-GFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. eGFPbait-E2A-KalTA4-pA donor vector

   Plasmid

   #61069

   Purpose

   for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

   Depositor

   Filippo Del Bene

   Article

   Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

   Inserts

   eGFPbait
   KalTA4
   

   Use

   Zebrafish expression

   Tags

   E2A

   Available Since

   Feb. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX-N-CTCF-mAID-Halo-donor

   Plasmid

   #139747

   Purpose

   Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

   Depositor

   Robert Tjian

   Article

   Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

   Insert

   CTCF CRISPR knock-in (Ctcf Mouse)
   

   Use

   CRISPR

   Tags

   mAID-HaloTag

   Available Since

   April 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. R26-H2B-mCherry HR donor vector

   Plasmid

   #137928

   Purpose

   R0SA26 knock-in vector with homologous recombination-based method

   Depositor

   Hiroshi Kiyonari

   Article

   Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

   Insert

   SA-H2B-mCherry-bpA with homology arms
   

   Use

   Mouse Targeting

   Available Since

   May 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. peSpCas9(1.1)-2×sgRNA (IFT88, donor)

   Plasmid

   #80769

   Purpose

   Expresses eSpCas9(1.1) and two sgRNAs. The first gRNA targets human IFT88 and the second gRNA targets a pDonor-tBFP-NLS-Neo (Universal).

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Insert

   IFT88 gRNA#1 (IFT88 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gata3-T2A-H2B-eGFP donor

   Plasmid

   #113119

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Gata3 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Gata3 donor region including homology arms and T2A-H2B-eGFP
   

   Use

   Synthetic Biology

   Available Since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Fgfr2-T2A-H2B-Halo donor

   Plasmid

   #113117

   Purpose

   Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Fgfr2 coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Fgfr2 donor region including homology arms and T2A-H2B-Halo
   

   Use

   Synthetic Biology

   Available Since

   Aug. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor

   Plasmid

   #211904

   Purpose

   Vector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.

   Depositor

   Yannick Doyon

   Article

   Levesque et al bioRxiv 2023.09.14.557485

   Insert

   CD19-CAR-28z-2A-EGFP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hPGK1

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pdgfra-T2A-H2B-eGFP donor

   Plasmid

   #113120

   Purpose

   Donor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequence

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   Pdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Mouse, Synthetic)
   

   Use

   Synthetic Biology

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. DHC1-AID-Clover donor (Hygro)

    Plasmid

    #158622

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. MADR pDonor smFP-HA (dark) WPRE

    Plasmid

    #131007

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of SM_FP-HA, a cytoplasmic fluorescent protein which includes multiple HA epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-HA WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    HA - 10 total HA epitope tages

    Expression

    Mammalian

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. MADR pDonor smFP-V5 (dark) WPRE

    Plasmid

    #131006

    Purpose

    MADR donor plasmid for FlpO+Cre- mediated insertion of a SM_FP-V5, a cytoplasmic fluorescent protein which includes multiple V5 epitope tags

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    smFP-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    V5 - 10 total of V5 epitope tag

    Mutation

    "dark" variant with GGG fluorphore comp…

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. DHC1-AID-Clover donor (Neo)

    Plasmid

    #158621

    Purpose

    Donor plasmid for tagging DHC1 with AID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    DHC1 (DYNC1H1 Human)
    

    Use

    CRISPR; Tagging donor

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. R26-EGFP MMEJ donor vector

    Plasmid

    #137926

    Purpose

    R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

    Depositor

    Hiroshi Kiyonari

    Article

    Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

    Insert

    SA-EGFP-bpA with micro-homology arms
    

    Use

    Mouse Targeting

    Available Since

    May 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MADR pDonor TagBFP2-V5-HRAS G12VWPRE

    Plasmid

    #129420

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic Hras G12V with a TagBFP2 fusion

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    TagBFP2-V5-HRAS G12V (HRAS Human, Entacmaea quadricolor (TagBFP))
    

    Use

    Mosaic analysis for dual recombinase-mediated cas…

    Tags

    TagBFP2-V5 tag

    Mutation

    G12V

    Promoter

    none (3x polyA to mitigate episomal expression)

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHiUGE Myc-epitope donor ORF0

    Plasmid

    #200374

    Purpose

    Tagging endogenously expressed proteins with Myc epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Myc epitope
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHiUGE Myc-epitope donor ORF1

    Plasmid

    #200375

    Purpose

    Tagging endogenously expressed proteins with Myc epitope, C-terminal

    Depositor

    Scott Soderling

    Article

    Gao et al Neuron. 2019 Aug 21;103(4):583-597.e8. d

    Insert

    Myc epitope
    

    Expression

    Mammalian

    Mutation

    NA

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Dppa1-T2A-H2B-Halo donor

    Plasmid

    #113118

    Purpose

    Donor plasmid for knock-in T2A-H2B-Halo to the c-terminal of mouse Dppa1 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Dppa1 donor region including homology arms and T2A-H2B-Halo
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAVS1 ObLiGaRe Donor vector/EPB58

    Plasmid

    #90016

    Purpose

    Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

    Depositor

    Eric-Paul Bennett

    Article

    Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

    Insert

    MCS flanked by inverted ZFN binding sites (PPP1R12C Human)
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. V954 - pDNR MCS SA no SA CmR - Donor

    Plasmid

    #11697

    Depositor

    Tony Pawson

    Article

    Colwill et al BMC Biotechnol. 2006 Mar 6;6:13. doi: 10

    Type

    Empty backbone

    Use

    Cre/Lox; Donor vector

    Available Since

    May 24, 2006

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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