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pHCMM1

Plasmid

#167452

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

TEF1

Availability

Academic Institutions and Nonprofits only

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pHCMM2

Plasmid

#167453

Purpose

Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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pHCMM3

Plasmid

#167454

Purpose

A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

Depositor

Hugo Cesar Medina-Munoz, Marvin Wickens

Publication

Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

PSEC63_yeGFP_cePUP-2_URA3_TADH1

Expression

Yeast

Promoter

PSEC63

Availability

Academic Institutions and Nonprofits only

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ρ1-EM GABAA receptor in pEZT-BM

Plasmid

#213072

Purpose

Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD

Depositor

Erik Lindahl

Publication

Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.

Insert

Human GABAa rho1 receptor (GABRR1 Human)

Use

Bacmam, baculovirus, oocyte expression

Tags

Twin-Strep tag and superfolderGFP

Expression

Mammalian

Mutation

Encodes residues S58–L383 and D451–S479, separate…

Promoter

CMV and T7

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-BFP-ecDHFR-NES-SV40polyA

Plasmid

#252089

Purpose

Expresses BFP-ecDHFR-NES in mammalian cells

Depositor

Karl Deisseroth

Publication

Zhao et al Nat Chem. 2026 Jul 8. doi: 10.1038/s4155

Insert

BFP-ecDHFR-NES

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pCMV-FKBP12F36V-eGFP-NMNAT1-SV40polyA

Plasmid

#252090

Purpose

Expresses FKBP12F36V-eGFP-NMNAT1 in mammalian cells

Depositor

Karl Deisseroth

Publication

Zhao et al Nat Chem. 2026 Jul 8. doi: 10.1038/s4155

Insert

FKBP12F36V-eGFP-NMNAT1

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP35

Plasmid

#250142

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP36

Plasmid

#250143

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pAAP37

Plasmid

#250144

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D2-IDR-RGG) (pgl-3 Nematode)

Tags

MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK042

Plasmid

#250139

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-IDR) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pRK082

Plasmid

#250140

Purpose

Expression of truncated PGL-3 in E. coli

Depositor

Geraldine Seydoux

Publication

Kuroiwa et al EMBO Rep. 2026 Mar 20. doi: 10.1038/s443

Insert

PGL-3(D1-D2-RGG) (pgl-3 Nematode)

Tags

6xHis::MBP::6xHis::TEV cleavage site

Expression

Bacterial

Available since

March 31, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC pdzd-8

Plasmid

#227844

Purpose

Used for transfection of cells for expression of C-MYC pdzd-8

Depositor

Sheng-cai Lin

Publication

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

pdzd-8 (C53B4.4 Nematode)

Tags

MYC

Expression

Mammalian

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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RT-5

Plasmid

#220442

Purpose

Construct used to generate the PUF3/PUP2/3HA tagging strains. (aka pBSII/PUF3 5’ flank/PUP2-DADA/3HA/URA3/PUF3 3’ flank)

Depositor

Marvin Wickens

Publication

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Insert

PUF3 (puf-3 Nematode)

Expression

Yeast

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHIT239

Plasmid

#204522

Purpose

In vitro transcription of Mos1 transposase mRNA with SL1 and poly(A) used for microinjection into nematode

Depositor

Yuji Kohara

Publication

Tabara et al EMBO J. 2023 Jun 1;42(11):e105002. doi:

Insert

Mos1_transposase::glh-2_3'UTR (glh-2 Nematode)

Use

Other

Promoter

T7 (minimum seq.)

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pdestMB14_grh-1p-grh-1-gfp

Plasmid

#177761

Purpose

Overexpression of C. elegans grh-1

Depositor

Michael Ristow

Publication

Grigolon et al Nat Commun. 2022 Jan 10;13(1):107. doi:

Insert

grh-1 (grh-1 Nematode)

Expression

Worm

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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TU#1412_tiam-1_cDNA

Plasmid

#82364

Purpose

express tiam-1 cDNA

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

tiam-1 cDNA (tiam-1 Nematode)

Expression

Worm

Promoter

none

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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TU#1449_unc-73e_cDNA

Plasmid

#82363

Purpose

express unc-73e cDNA

Depositor

Martin Chalfie

Publication

Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

Insert

unc-73 e isoform (unc-73 Nematode)

Expression

Worm

Promoter

none

Available since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only
pDMG7a

Plasmid

#33176

Depositor

David Bartel

Publication

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

T05C12.8 (T05C12.8 Nematode)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pJW2332

Plasmid

#253923

Purpose

linker::mNeonGreen::3xFLAG::linker with unique crRNA sites

Depositor

Jordan Ward

Publication

Ragle et al bioRxiv [Preprint]. 2025 May 14:2025.05.

Insert

12aa linker::mNeonGreen::TEV::3xFLAG::12aa linker

Expression

Worm

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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pJW2471

Plasmid

#253925

Purpose

linker::mScarlet (GLO)::3xHA::linker for re-editing knock-in strains

Depositor

Jordan Ward

Publication

Ragle et al bioRxiv [Preprint]. 2025 May 14:2025.05.

Insert

12aa linker::mScarlet (GLO)::3xHA::12aa linker

Expression

Worm

Available since

May 27, 2026

Availability

Academic Institutions and Nonprofits only

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