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pHES841

Plasmid

#89194

Purpose

Expresses YFP under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Expression

Yeast

Promoter

pZ

Available Since

Jan. 10, 2018

Availability

Academic Institutions and Nonprofits only

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LZF80: Cre-on CAG-somCheRiff-CFP (DIO)

Plasmid

#104115

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

somCheRiff-CFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only
Rap-MESA-20-M-Gal4

Plasmid

#84554

Purpose

MESA target chain with rapamycin FRB ectodomain, 20 extracellular linkers, M cleavage sequence, and Gal4 transactivator

Depositor

Joshua Leonard

Article

Hartfield et al ACS Synth Biol. 2017 Nov 17;6(11):2042-2

Insert

Rap-MESA-FRB-20-M-Gal4

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pHES874

Plasmid

#89196

Purpose

Expresses mCherry under control of synthetic promoter containing Zif268 binding sites.

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-mCherry

Expression

Yeast

Promoter

pZ

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB602-mCherry-sfGFP

Plasmid

#213860

Purpose

For Agrobacterium transformation. mCherry-sfGFP under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.

Insert

mCherry-sfGFP

Expression

Bacterial and Plant

Promoter

CaMV 35S

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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pF3BGX-mScarlet-I

Plasmid

#138391

Purpose

RMCE vector for generating C-terminal mScarlet-I fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

mScarlet-I

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-NHERF1-EB

Plasmid

#28297

Depositor

Maria-Magdalena Georgescu

Article

Morales et al Mol Cell Biol. 2007 Apr . 27(7):2527-37.

Insert

NHERF1 (Nherf1 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Contains only the EB domain aa 262-355 (deletion …

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
pF hPGK mc2 IRES neo

Plasmid

#206236

Purpose

For expressing cloned exons as circular RNA.

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Sept. 1, 2023

Availability

Academic Institutions and Nonprofits only

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MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pRL8

Plasmid

#24980

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2010 Jun;7(6):451-3. doi: 1

Insert

Cbr-unc-119

Use

Multisite gateway

Available Since

Aug. 4, 2010

Availability

Academic Institutions and Nonprofits only

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MTK3a_007

Plasmid

#123760

Purpose

Encodes Peroxisome Targeting Sequence as a Type 3a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PTS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Rnd2

Plasmid

#23228

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd2 (RND2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pET14b_SG

Plasmid

#59737

Purpose

Expresses SH3-GUK domains of human ZO-1

Depositor

Alan Fanning

Article

Lye et al J Biol Chem. 2010 Apr 30;285(18):13907-1

Insert

zonula occludens-1 (TJP1 Human)

Tags

HIS tag and thrombin clvg

Expression

Bacterial

Available Since

Nov. 5, 2014

Availability

Academic Institutions and Nonprofits only
LoriT

Plasmid

#163691

Purpose

Modified L4440 containing now CamR and oriT for conjugation

Depositor

Baris Tursun

Article

Kazmierczak et al Nucleic Acids Res. 2021 Feb 26;49(4):e22

Insert

oriT

Expression

Bacterial

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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XW54

Plasmid

#65839

Purpose

Punc-4c-NLS-QF-BD-DM-Nzipper-SL2-mCherry-3'UTR-unc-54 for Split Q intersectional method

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

NLS-QF-BD-DM-Zipper

Tags

mCherry

Expression

Worm

Promoter

Punc-4c

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G:GlyR-IRES-GFP

Plasmid

#32482

Purpose

Expresses PSAM-GlyR (Q79G) neuronal inhibitor, with IRES-EGFP marker

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G-GlyR

Tags

IRES-EGFP

Expression

Mammalian

Promoter

CAG

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pTGMP-p16/p19.478

Plasmid

#32717

Depositor

Scott Lowe

Article

Premsrirut et al Cell. 2011 Apr 1;145(1):145-58.

Insert

p16/p19

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Aug. 1, 2012

Availability

Academic Institutions and Nonprofits only
pET11-His6-Pif6-sfGFP-ssrA

Plasmid

#140891

Purpose

Expression of PIF-sfGFP-ssrA in E. coli

Depositor

Neal Devaraj

Article

Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

PIF-sfGFP-ssrA

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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XW99

Plasmid

#65842

Purpose

MosSCI-Pmig-13::QS::SL2::mCherry for ttTi4348 on Chrom I.

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Tags

mCherry

Expression

Worm

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL4

Plasmid

#138392

Purpose

RMCE vector for generating C-terminal T2A-GAL4 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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