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pENTR-SYNZIP19

Plasmid

#80673

Purpose

Encodes the synthetic coiled coil peptide SYNZIP19

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP19

Use

Entry vector

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pGGH000

Plasmid

#48862

Purpose

Empty entry vector for creating GreenGate N-terminal tag + coding sequence + C-terminal tag + terminator modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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R777-E102 Hs.HRAS-nostop

Plasmid

#70386

Purpose

Gateway ORF clone of human HRAS [NM_005343.2] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

HRAS (HRAS Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBD28

Plasmid

#245766

Purpose

Allows for tiam-1::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

tiam-1::mGFP

Use

Unspecified

Tags

mGFP

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pSJC317

Plasmid

#245773

Purpose

Allows for rac-2::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rac-2::mGFP

Use

Unspecified

Tags

mGFP

Available Since

Sept. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pBD24

Plasmid

#245764

Purpose

Allows for rap-1::mCherry entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rap-1::mCherry

Use

Unspecified

Tags

mCherry

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pBD25

Plasmid

#245765

Purpose

Allows for rap-1::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

rap-1::mGFP

Use

Unspecified

Tags

mGFP

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pMpGWB303-MpIGPDm

Plasmid

#188555

Purpose

Binary vector for MpIGPDm and mALS as selective markers in Marchantia polymorpha (igpd mutants)

Depositor

Yutaka Kodama

Article

Fukushima et al Plant Biotechnol (Tokyo). 2022 Dec 25;39

Insert

MpIGPDm and mALS

Use

CRISPR; Entry vector

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Aq(1st)

Plasmid

#190917

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use as the first 2A peptide in quad-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_O2Ab

Plasmid

#183083

Purpose

2A peptide O2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

O2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pYTK001_P2Ab

Plasmid

#183084

Purpose

2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use in bi-cistronic and as the first 2A peptide in tri-cistronic construct assembly

Depositor

Zhen Wang

Article

Mukherjee et al Biotechnol Bioeng. 2022 Sep 27. doi: 10.

Insert

P2A

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Magenta

Plasmid

#183140

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Magenta

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Magenta

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Yellow

Plasmid

#183141

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Yellow

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Yellow

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Orange

Plasmid

#183143

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Orange

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1_Pink

Plasmid

#183142

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1_Pink

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2364-Tier1-IRES

Plasmid

#169562

Purpose

Tier-1 vector carrying an internal ribosome entry site (IRES-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Type

Empty backbone

Expression

Mammalian

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1

Plasmid

#160448

Purpose

BioBrick mammalian-codon-optimized mRFP1

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-dsRed

Plasmid

#160449

Purpose

BioBrick mammalian-codon-optimized dsRed

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, dsRed

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGD252

Plasmid

#132373

Purpose

contains the sequence of TSapphire for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-TSapphire

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pGD254

Plasmid

#132375

Purpose

contains the sequence of mOrange for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

mOrange

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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