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pXPR_003-sgBIRC6-1

Plasmid

#202437

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_016-sgHRI

Plasmid

#202443

Purpose

Expression of a guide RNA targeting HRI

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

HRI gRNA (EIF2AK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_023d-sgCiBIRC6-5

Plasmid

#202448

Purpose

Expression of a CRISPRi guide targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgBIRC6-4

Plasmid

#202438

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pXPR_003-sgBIRC6-5

Plasmid

#202439

Purpose

Expression of a guide RNA targeting BIRC6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

BIRC6 gRNA (BIRC6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pXPR_003-sgUBA6

Plasmid

#202440

Purpose

Expression of a guide RNA targeting UBA6

Depositor

William Hahn

Article

Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

Insert

UBA6 gRNA (UBA6 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/shCol1a1/Scarlet_Seq1.2

Plasmid

#201404

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet-Seq1.1

Plasmid

#201401

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-S212D

Plasmid

#197515

Purpose

Mammalian expression of myc-tagged human Nix with an aspartic acid substitution of serine-212. Based on Myc-Nix (#100795)

Depositor

Joseph Gordon

Article

da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Mutation

Serine 212 changed to aspartic acid

Promoter

CMV

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-S212A

Plasmid

#197514

Purpose

Mammalian expression of myc-tagged human Nix with an alanine substitution of serine-212. Based on Myc-Nix (#100795)

Depositor

Joseph Gordon

Article

da Silva Rosa et al Autophagy. 2021 Sep;17(9):2257-2272. doi

Insert

BCL2 interacting protein 3 like (BNIP3L Human)

Tags

Myc

Expression

Mammalian

Mutation

Serine 212 changed to alanine

Promoter

CMV

Available Since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Downstream_gRNA_2

Plasmid

#195137

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 2 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Downstream_gRNA_1

Plasmid

#195136

Purpose

gRNA in a third generation gRNA backbone with mCherry, targeting region immediately downstream of MDM4, to be used with MDM4_Deletion_Upstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Downstream gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Upstream_gRNA_2

Plasmid

#195135

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 2 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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Chr1q_Cassette-Proximal_gRNA

Plasmid

#195127

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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MDM4_Deletion_Upstream_gRNA_1

Plasmid

#195134

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting region immediately upstream of MDM4, to be used with MDM4_Deletion_Downstream_gRNA_1/2 for MDM4 deletion

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

MDM4 Deletion Upstream gRNA 1 (MDM4 Human)

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Dest-MBP-Nsp1

Plasmid

#191483

Purpose

Mammalian expression of MBP-tagged SARS-CoV-2 Nsp1

Depositor

Hao Wu

Article

Vora et al Proc Natl Acad Sci U S A. 2022 Mar 1;119

Insert

MBP-Nsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Nov. 15, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_219

Plasmid

#180534

Purpose

Entry vector containing K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

K.rhaeticus native promoter pCmcAX (bcs1) (200 bases upstream from gene start codon)

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_223

Plasmid

#180538

Purpose

Entry vector containing K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

K.rhaeticus native promoter pHxu (200 bases upstream from gene start codon)

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
KTK_226

Plasmid

#180552

Purpose

Entry vector containing K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

K.rhaeticus native promoter pRutB (200 bases upstream from gene start codon)

Expression

Bacterial

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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